Method for measuring Farnesal contents in medicine loading micelle by high performance liquid chromatography

A technology of high performance liquid chromatography and drug-loaded micelles, which is applied in the field of drug analysis to achieve the effects of strong specificity, easy operation, and simple operation

Inactive Publication Date: 2019-06-14
SOUTHWEST MEDICAL UNIVERISTY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] After retrieval, there is no simple, efficient, specific, high-accuracy, and reproducible method for in vitro determination of farnesaldehyde content in the prior art

Method used

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  • Method for measuring Farnesal contents in medicine loading micelle by high performance liquid chromatography
  • Method for measuring Farnesal contents in medicine loading micelle by high performance liquid chromatography
  • Method for measuring Farnesal contents in medicine loading micelle by high performance liquid chromatography

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Embodiment 1

[0046] The method for determining the content of farnesaldehyde in drug-loaded micelles by high performance liquid chromatography of the present embodiment adopts Agilent-1260 high performance liquid chromatography, comprising the following steps:

[0047] (1) Selection of detection wavelength

[0048] Accurately weigh 5 mg of farnesal (Farnesal, Far), place it in a 25 mL volumetric flask, add acetonitrile to dissolve it completely, and constant volume to obtain farnesal stock solution (100 μg / mL). Measure a small amount of farnesaldehyde stock solution, dilute it with acetonitrile to an appropriate concentration, and use acetonitrile as a blank control, and use a UV spectrophotometer to scan in the wavelength range of 200-600nm to determine the maximum absorption wavelength.

[0049] Through the full-wavelength scanning of the ultraviolet spectrophotometer, 216nm was selected as the UV detection wavelength.

[0050] (2) Chromatographic conditions

[0051] In this experiment...

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Abstract

The invention relates to a method for measuring Farnesal contents in medicine loading micelle by high performance liquid chromatography, and belongs to the field of medicine analysis. An Agilent-1260high performance liquid chromatograph and an ultraviolet detector are adopted, a detection wavelength is 216nm, and a chromatographic condition is that a chromatographic column is a reverse chromatographic column which selects octadecylsilane chemically bonded silica as a filler; a moving phase is preferably mixture of acetonitrile and water, wherein the volume ratio of acetonitrile to water is 80:20; and a flow rate is 0.8-1.2mL/min, a sample introduction amount is 5-15muL, and a column temperature is 20-40DEG C. The method has the characteristics of being simple in operation, high in specificity, high in accuracy, good in precision and the like, and an intra-day and daytime standard deviation does not exceed 1.2%. Therefore, by use of the method, the requirement that Farnesal contents can be accurately and effectively measured can be met, and the method can be accurately used for testing Farnesal contents in experiments, including the encapsulation efficiency, the medicine loading capacity, the in vitro release and the like of the Farnesal-loaded medicine loading micelle.

Description

technical field [0001] The invention belongs to the field of drug analysis, more specifically, the invention relates to a method for determining the content of farnesaldehyde in drug-loaded micelles by high performance liquid chromatography. Background technique [0002] Farnesal (Farnesal, Far), also known as farnesal, belongs to sesquiterpene aldehydes, mainly from lemongrass and verbena, and its chemical name is: 3,7,11-trimethyl-2,6 ,10-Dodecatrienal, the molecular formula is C 15 h 24 O, the molecular weight is 220.35. Farnesal has poor water solubility and is almost insoluble in water. This product can be prepared by oxidation or dehydrogenation of farnesal. Studies have shown that farnesal is non-toxic in vivo and can inhibit the growth of various bacterial microorganisms, such as Staphylococcus aureus, Staphylococcus epidermidis and Candida albicans. In addition, farnesal can also affect the permeability of Streptococcus mutans biofilm and reduce the glycolysis o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 林燕钟志容刘中兵易佑平
Owner SOUTHWEST MEDICAL UNIVERISTY
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