1 results about "Peramivir" patented technology

This invention relates to the field of pharmaceutical analysis technology, and provides a method for detecting enantiomers in peramivir sodium chloride injection. The method employs high-performance liquid chromatography (HPLC), with the following chromatographic conditions: a column packed with α1-acidic glycoprotein-bonded silica gel; a mobile phase consisting of a mixture of 0.02 mol / L ammonium dihydrogen phosphate solution and 0.02 mol / L diammonium hydrogen phosphate solution at a volume ratio of 45:55 to 55:45; a detection wavelength of 205 nm; a flow rate of 0.25 to 0.35 mL / min; and a column temperature of 18 to 22 °C. Before detection, a test solution, a reference solution, and a system suitability solution are prepared, and each solution is accurately injected into the HPLC system. The chromatograms are recorded, and the enantiomer content is calculated. The advantages of this invention are: simple operation, no sodium chloride interference, and accurate quantitative detection of enantiomers in peramivir sodium chloride injection.