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Application of peramivir in the preparation of drugs for treating inflammatory storm caused by infectious diseases

A technology for infectious diseases and inflammation, applied in the field of medicine, can solve problems such as no clear report on the clinical application of peramivir, and achieve the effect of reducing the secretion of cytokines and inhibiting inflammatory storms

Active Publication Date: 2022-08-09
THE NAVAL MEDICAL UNIV OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no clear report on the clinical application of peramivir as a drug to inhibit the inflammatory storm caused by infectious diseases

Method used

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  • Application of peramivir in the preparation of drugs for treating inflammatory storm caused by infectious diseases
  • Application of peramivir in the preparation of drugs for treating inflammatory storm caused by infectious diseases
  • Application of peramivir in the preparation of drugs for treating inflammatory storm caused by infectious diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Establishment of LPS-stimulated mouse peritoneal macrophage model:

[0030] 1.1 Materials

[0031] C57BL / 6 strain mice, male, weighing 18-22g, purchased from Changzhou Cavens Laboratory Animal Co., Ltd., production license number SCXK (Su) 2016-0010; LPS derived from Escherichia coli 0111:B4 purchased from Sigma-Aldrich Company; RPMI-1640 medium, fetal bovine serum and double antibody are products of Gibco Company; CCK8 was purchased from Tongren Institute of Chemistry; TNFα Elisa detection kit was purchased from Invitrogen Company (Cat. No.: BMS607-3TEN); Microplate reader was BioTek brand.

[0032] 1.2 Isolation and culture of mouse peritoneal macrophages

[0033] C57BL / 6 mice were intraperitoneally injected with 3 ml of broth medium (purchased from BD) containing 3% mercaptosulfate. After 3 days, they were sacrificed by cervical dislocation and soaked in 75% alcohol twice for 1 minute each time. The mouse was then placed on a sterile operating table, and the abdomi...

Embodiment 2

[0041] Establishment of LPS-induced CSS mouse model:

[0042] 1.1 Materials

[0043] C57BL / 6 strain mice, male, weighing 18-22g, purchased from Changzhou Cavens Laboratory Animal Co., Ltd., production license number SCXK (Su) 2016-0010; LPS derived from Escherichia coli 0111:B4 purchased from Sigma-Aldrich Company; multi-factor detection kits were purchased from BioLegend (Mouse Anti-Virus Panel, Cat No: 740622; MouseInflammation Panel, Cat No: 740446); CytoFlex flow cytometer was the Beckman Coulter brand of the United States.

[0044] 1.2 Animal models

[0045] LPS was selected by intraperitoneal injection of 15 mg / kg in mice to induce CSS.

[0046] 1.3 Plasma separation

[0047] 4 hours after LPS injection, blood was collected from the orbit to an anticoagulant tube containing EDTA, and after standing for 30 minutes, centrifuged at 4000 rpm for 15 minutes at 4 °C, and the supernatant, that is, plasma, was transferred to an EP tube for use.

[0048] 1.4 Collection of mou...

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Abstract

The invention discloses an application of peramivir in preparing a medicine for treating an inflammatory storm caused by an infectious disease, wherein the infectious disease is an infection caused by a virus, chlamydia, mycoplasma, bacteria or parasite, and the inflammatory storm is Infectious diseases cause the rapid and massive production of various cytokines in the body, and the various cytokines in the body are TNFα, IL-6, IL-1β, IL-12, IFNα and IFN-γ. The present invention proves for the first time that peramivir has the effect of treating inflammatory storm caused by infectious disease, can be used for preparing the therapeutic drug for inflammatory storm caused by infectious disease, and provides a new anti-inflammatory scheme for clinical treatment.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of peramivir in the preparation of a medicine for treating inflammatory storms caused by infectious diseases. Background technique [0002] Infectious diseases refer to diseases caused by patients infected by various pathogens such as viruses, chlamydia, mycoplasmas, bacteria or parasites. As the infection continues to intensify, the immune system produces a large amount of cytokines, among which pro-inflammatory cytokines can chemotactic more immune cells to form a positive feedback loop, causing an "inflammatory storm", attacking the human body indiscriminately. Damage to the immune system and vital organs such as the liver, kidneys, and heart. "Inflammatory storm", also known as cytokine storm syndrome (CSS), refers to the rapid development of a variety of cytokines such as TNFα, IL-1β, IL-6 and MCP-1 in body fluids after the body is infected with...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/196A61P29/00A61P31/12A61P31/04A61P33/00
CPCA61K31/196A61P29/00A61P31/12A61P31/04A61P33/00Y02A50/30
Inventor 王志斌庄春林苏笠
Owner THE NAVAL MEDICAL UNIV OF PLA
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