Extraction, purification and conversion of flavonoids from plant biomass

A compound, flavonoid technology, applied in the field of extraction, purification and conversion of flavonoids from plant biomass

Inactive Publication Date: 2005-10-19
加拿大农业部
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is currently no commercially viable technology to bioconvert rutin (regardless of origin) into highly bioavailable, performance-enhancing, high-value products such as isoquercitrin and quercetin

Method used

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  • Extraction, purification and conversion of flavonoids from plant biomass
  • Extraction, purification and conversion of flavonoids from plant biomass
  • Extraction, purification and conversion of flavonoids from plant biomass

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0137] Example 1: Aqueous extraction, concentration and precipitation of rutin from buckwheat leaf material

[0138] After harvesting and drying, buckwheat leaves were ground on a Wiley mill to pass through a 2mm sieve, thereby preparing buckwheat leaves for extraction. Extraction was carried out by 1 kg of ground buckwheat leaves (3.74% rutin content, based on dry weight) in 10 L of water with continuous stirring at 90° C. for 1 hour. The resulting suspension was filtered, and the filter cake was washed twice with 300 ml of hot (95° C.) water. The washed filtrate was mixed with the extract to obtain a combined extract with a volume of 8.6 L. This aqueous extraction method recovered 36% of the available rutin from the leaves.

[0139] The extract was concentrated under reduced pressure to about 1 / 5 and 1 / 10 of its original volume. The concentrated extract was stored in the refrigerator (4°C) overnight, at which time the flavonoid compounds precipitated out of solution. The...

Embodiment 2

[0140] Example 2: Aqueous Alcoholic Extraction, Concentration and Precipitation of Rutin from Buckwheat Leaf Material

[0141] After harvesting and drying, buckwheat leaves were ground on a Wiley mill to pass through a 2mm sieve, thereby preparing buckwheat leaves for extraction. 1 kg of ground buckwheat leaves (rutin content 3.74%, based on dry weight) were extracted in 10 L of 50% (v / v) aqueous methanol, specifically with continuous stirring at 40° C. for 3 hours. The resulting suspension was filtered, and the filter cake was washed with warm (40%) 50% (v / v) aqueous methanol. The washed filtrate was combined with the extract. This extraction method recovered 65% of the available rutin from the leaves. Figure 2A illustrates the concentration of rutin in methanolic extracts. The extract was concentrated to about 1 / 5 of its original volume under reduced pressure. The concentrated extract was stored in the refrigerator (4°C) overnight, at which time the flavonoid compounds p...

Embodiment 3

[0142] Example 3: Purification of rutin from a flavonoid-rich intermediate isolated from buckwheat leaves

[0143] The rutin-enriched product from Example 2 was dissolved in warm methanol with vigorous stirring on a magnetic stirrer to promote complete dissolution of the rutin. Any insoluble material was removed from the solution using vacuum filtration. The solution was evaporated to dryness at 40°C under reduced pressure. The residue was then suspended in hot (90°C) water with continuous stirring until most of the precipitate had dissolved. Allow the suspension to settle overnight in the refrigerator. The precipitate was removed by vacuum filtration and freeze-dried. The purified rutin precipitate was dissolved in methanol, filtered through a 0.45 μm nylon syringe filter, and analyzed by RP-HPLC to confirm product purity. After repeated dissolution / crystallization without chromatography, the rutin content increased to about 70% and higher.

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Abstract

A process for preparing a rutin-enriched composition from plant biomass comprises extraction with an aqueous solution, and precipitation. An enzyme preparation, such as naringinase, is used for the transformation of rutin to higher value compositions containing increased proportions of isoquercitrin and quercetin.

Description

[0001] references [0002] Abou-Karam, M., and Shier, W.T. 1992. Isolation and characterization of antiviral flavonoids from Waldsteinia fragarioides. J.Nat.Prod.55:1525-1527. [0003] Agullo, G., Gamet, L., Besson, C., Demigne, C., and Remesy, C. 1994. Quercetin exerts preferential cytotoxic effects on actively dividing colon cancer HT29 and CaCa-2 cells. Cancer Letters .87:55-63. [0004] Agullo, G., Gamet-Payrastve, L., Manenti, S., Viala, C., Remesy, C., Chap, H., and Payrastve, B. 1997. Structure of flavonoid compounds and phosphatidylinositol 3- The relationship between kinase inhibition: a comparison of tyrosine kinase and protein kinase C inhibition. Biochem. Pharmacology. 53: 1649-1657. [0005] Anonymous. 1990a. Hayashibara Biochemistry: Assignment of high concentrations of water-soluble rutin to samples. New Technology Japan. February. 34 p. [0006] Anonymous. 1990b. Water-soluble rutin that functions as vitamin P. Japan Report Medical Technology. Feb. [0007] A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/30A61K8/60A61Q17/04A61Q19/08C12P17/06C12P19/60
CPCA23L1/3002A61Q19/08C12P19/60C12P17/06A61Q17/04A61K31/7048A61K8/602A23L33/105A61P3/04A61P3/06A61P3/10A61P9/00A61P9/10A61P9/12A61P11/06A61P25/16A61P25/28A61P29/00A61P31/12A61P35/00A61P39/06A61P43/00
Inventor P·R·昌A·缪尔
Owner 加拿大农业部
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