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Rutin-zein-sodium caseinate composite nanoparticles and preparation method thereof

A technology of zein and composite nanoparticles, which is applied in the direction of drug combinations, pharmaceutical formulas, non-effective ingredients of polymer compounds, etc., can solve the problem that degradable nanoparticle materials are not easily accepted by consumers, the safety of protein nanoparticles is reduced, chemical Cross-linking agent is toxic and other problems, to achieve good embedding effect, small particle size, uniform distribution effect

Inactive Publication Date: 2017-05-31
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since rutin is insoluble in water, it is greatly limited when it is added to food as a good new anti-free radical food additive
Although the traditional technology of embedding rutin into composite particles has solved the solubility problem of rutin, there are still the following problems: 1. The particle size of the microspheres is too large to be dispersed and suspended in the liquid system; 2. The synthesis can Degraded nanoparticle materials are not easily accepted by consumers; 3. Among natural nanoparticle materials: lipids cannot embed active ingredients well due to their long-chain structure; proteins need to add chemical cross-linking agents due to their strong hydrophilicity. Covalent bonds are formed between proteins to prevent the disintegration of the nanoparticle structure. Most chemical cross-linking agents are toxic, and the safety of protein nanoparticles with cross-linking agents is greatly reduced.

Method used

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  • Rutin-zein-sodium caseinate composite nanoparticles and preparation method thereof
  • Rutin-zein-sodium caseinate composite nanoparticles and preparation method thereof
  • Rutin-zein-sodium caseinate composite nanoparticles and preparation method thereof

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Embodiment 1

[0035] 1. Preparation and yield, particle size, Z-potential, PDI and embedding rate characteristics of rutin-zein-sodium caseinate composite nanoparticles (CN-ZR nanoparticles)

[0036] 1. Preparation method of CN-ZR nanoparticles

[0037] Adopt anti-solvent method to prepare CN-ZR nanoparticles, the steps are as follows:

[0038] (1) Dissolve 0.1 g of rutin and 1.0 g of zein in 20 mL of ethanol-water solution, and the volume ratio concentration of ethanol in the ethanol-water solution is 60% to 90%.

[0039] (2) Then quickly add 50 mL of sodium caseinate solution to the mixed solution in step (1). The mass ratio concentration of the sodium caseinate solution is 1%-2.5%.

[0040] (3) The mixture obtained in step (2) was rotatated at 45° C. for 10 minutes, centrifuged at 4000 rpm for 10 minutes to remove a small amount of insoluble matter, and then freeze-dried for 24 hours. The obtained lyophilized powder samples were stored at 4°C until assayed.

[0041] 2. Yield

[0042] ...

Embodiment 2

[0077] The SEM scanning electron microscope of embodiment 2, CN-ZR nanoparticle, infrared spectrogram, color, slow release ability and antioxidant capacity are measured

[0078] 1. The appearance of nanoparticles SEM

[0079] Scanning electron microscopy was used to observe the appearance of nanoparticles. First, a nanoparticle solution with a mass ratio of 0.1-0.5% was prepared. After ultrasonic treatment at room temperature for 5 minutes, the metal foil was dipped into the solution and put on the stage, and quickly freeze-dried for observation. The result is as figure 2 shown. Depend on figure 2 It can be seen from A and B that the zein nanoparticles without adding sodium caseinate and rutin present a uniform spherical shape and a uniform particle size ( about), and after adding sodium caseinate, the nanoparticles are partially aggregated, and particles with smaller particle sizes appear, and the average particle size drops to about 253nm in diameter (such as figure...

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Abstract

The invention provides rutin-zein-sodium caseinate composite nanoparticles and a preparation method thereof. The rutin-zein-sodium caseinate composite nanoparticles are prepared by taking rutin as an antioxidant, taking zein as a carrier and taking sodium caseinate as a stabilizer through an anti-solvent method. Instruments such as a laser particle analyzer, a Fourier transform infrared spectrum analyzer and a scanning electron microscope (SEM) are adopted for analyzing the representational structure of the nanoparticles, and the yield, the controlled-release rate and oxygen resistance are measured. As proved by a result, the composite nanoparticles have a very good embedding effect on the rutin, have small particle sizes, and are uniformly distributed in solutions; as indicated by an SEM picture, the composite nanoparticles are spherical, and the particle sizes are uniform in distribution; as indicated by the analysis of the Fourier transform infrared spectrum analyzer, hydroxyl radicals in nanoparticle molecules are increased; moreover, the prepared nanoparticles have a certain controlled-release proportion and high oxygen resistance.

Description

technical field [0001] The invention belongs to the technical field of nanoparticle application, and in particular relates to a rutin-zein-sodium caseinate composite nanoparticle and a preparation method thereof. Background technique [0002] Nanoparticles generally refer to solid particles with a particle size of less than 1 μm. Micron-sized particles are difficult to disperse and suspend in liquid systems due to gravity. Nanoparticles, on the other hand, have good stability, and their suspensions can remain stable for up to three months. In the test of the intestinal model, the absorption rate of 100nm nanoparticles is 15-250 times that of 1-10μm, because nanoparticles can penetrate the submucosal layer while microspheres can only stay on the epidermis. Therefore, nanoparticles have obvious advantages over micron-sized particles in terms of stability and physiological availability. Nanoparticles can be used in a wide range of applications, including catalytic reactions,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K47/42A61K31/7048A61P39/06
CPCA61K9/5169A61K31/7048
Inventor 张双灵
Owner QINGDAO AGRI UNIV
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