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Content determination method for Sedum aizoon medicinal material and product thereof

A determination method and content technology, which is applied in the field of detection of Chinese herbal medicines, can solve problems such as undefined product content detection methods and unfavorable product quality, and achieve the effects of good linear relationship, good instrument precision, and quality assurance

Pending Publication Date: 2022-02-11
贵州中医药大学
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above method isolated 13 compounds from the freeze-dried powder of June Huanyang fresh medicine, but there is no clear method for product content detection, so it is not conducive to ensuring product quality

Method used

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  • Content determination method for Sedum aizoon medicinal material and product thereof
  • Content determination method for Sedum aizoon medicinal material and product thereof
  • Content determination method for Sedum aizoon medicinal material and product thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Preparation of the test solution: Accurately weigh 0.5g of the June Huanyang sample powder, put it in a stoppered Erlenmeyer flask, accurately add 20mL of 60% methanol solution, weigh it, and extract it by ultrasonic extraction with a power of 300W and a frequency of 40kHz After 20 minutes, let it cool, make up the lost weight with 60% methanol, shake well, and filter with 0.45μm filter membrane to get it;

[0032](2) Preparation of mixed reference substance solution: Accurately weigh appropriate amount of gallic acid, myricitrin, isoquercitrin, guajagarin, quercitrin, myricetin-3-O-(2"-galloyl)-α-L -Rhamnopyranoside, quercetin-3-O-(2"-galcoyl)-rhamnoside, dissolved in 60% methanol and formulated to contain gallic acid, myricetin, isoquercitrin, Guavaside, Quercitrin, Myricetin-3-O-(2"-galloyl)-α-L-rhamnopyranoside, Quercetin-3-O-(2"-galloyl)- Mixed reference solution with rhamnosides of 237.3, 239.6, 39.9, 63.4, 207.9, 419.3, 105.1 μg, respectively;

[0033] (3) ...

Embodiment 2

[0037] (1) Preparation of the test solution: Accurately weigh 0.5g of June Huanyang sample powder, put it in a stoppered Erlenmeyer flask, accurately add 10mL of 25% methanol solution, weigh it, and extract it by ultrasonic extraction with a power of 250W and a frequency of 30kHz Let it cool for 5 minutes, make up for the lost weight with 25% methanol, shake well, and filter with a 0.45 μm filter membrane to obtain the product;

[0038] (2) preparation of mixed reference substance solution: with embodiment 1;

[0039] (3) Chromatographic conditions and system suitability test: Chromatographic column: Atlantis T3 C18 column, size 4.6mm×150mm, 5μm; mobile phase: acetonitrile as mobile phase A, 0.10% phosphoric acid as mobile phase B, methanol as mobile phase C; Gradient elution program: 0min, 5%A, 90%B, 5%C; 8min, 14%A, 81%B, 5%C; 12min, 14%A, 81%B, 5%C; 27min, 20%A, 75%B, 5%C; 37min, 30%A, 60%B, 5%C, 38min, 5%A, 90%B, 5%C; 42min, 5%A, 90% B. 5% C; column temperature 30°C, flo...

Embodiment 3

[0042] (1) Preparation of the test solution: Accurately weigh 0.5g of the June Huanyang sample powder, put it in a stoppered Erlenmeyer flask, add 40mL of 100% methanol solution accurately, weigh the mass, and extract it by ultrasound with a power of 350W and a frequency of 50kHz After 30 minutes, let it cool, make up the lost weight with 100% methanol, shake well, and filter with 0.45μm filter membrane to get the product;

[0043] (2) preparation of mixed reference substance solution: with embodiment 1;

[0044] (3) Chromatographic conditions and system suitability test: Chromatographic column: Atlantis T3 C18 column, size 4.6mm×150mm, 5μm; mobile phase: acetonitrile as mobile phase A, 0.20% phosphoric acid as mobile phase B, methanol as mobile phase C; Gradient elution program: 0min, 5%A, 90%B, 5%C; 8min, 14%A, 81%B, 5%C; 12min, 14%A, 81%B, 5%C; 27min, 20%A, 75%B, 5%C; 37min, 30%A, 60%B, 5%C, 38min, 5%A, 90%B, 5%C; 42min, 5%A, 90% B. 5% C; column temperature 30°C, flow rat...

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Abstract

The invention discloses a method for determining the content of a Sedum aizoon medicinal material and a product thereof. The invention establishes a method for simultaneously determining the content of seven chemical components, namely gallic acid, myricetin, isoquercitrin, guaijaverin, quercitrin, myricetin-3-O-(2 '-gallyl)-alpha-L-rhamnopyranoside and quercetin-3-O-(2'-gallyl)-rhamnoside, in the Miao medicine Sedum aizoon. The method can be used for simultaneously determining seven chemical components in the Sedum aizoon, has the advantages of high determination efficiency, favorable precision, favorable reproducibility and favorable linear relationship, is stable and reliable, efficiently detects the content of effective components in June Reyang, and effectively ensures the product quality.

Description

technical field [0001] The invention relates to the technical field of detection of Chinese medicinal materials, in particular to a method for simultaneously determining the contents of seven components in June Huanyang by using HPLC. Background technique [0002] Miao medicine June Huanyang is the whole herb of Sedum aizoon L., a plant of the Sedum family. It is also known as Sedum Sanchi, Feicai, Jiuxincai, Sanbaibang, Tusanqi, etc. It is a commonly used medicinal material in the Miao area. [1] . Miao medicine June Huanyang is a medicine for mending leakage in traumatology medicine. It is cold and mild in nature, belongs to heat, and has fast menstruation. Its quality is sweet and bitter. It has the functions of calming the nerves and detoxification, and has a remarkable therapeutic effect on bruises or wounds on the body surface. It is often used in various bleeding syndromes in the folk, so it is also known as the "Holy Medicine for Traumatology" of the Miao nationality...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027Y02A50/30
Inventor 何席呈吴珊珊杜江张葭李婷赵学芬
Owner 贵州中医药大学
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