Method for separating and purifying hyperoside and isoquercitroside from aurea helianthus

A technology of hyperoside and isoquercitrin, which is applied in the field of extraction and separation of natural compounds, can solve the problems of cumbersome purification methods, difficult industrial production, and long production cycle, and achieve low cost, easy recycling, and reduced risk Effect

Active Publication Date: 2018-03-06
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have problems such as cumbersome purification methods, high cost, long production cycle

Method used

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  • Method for separating and purifying hyperoside and isoquercitroside from aurea helianthus
  • Method for separating and purifying hyperoside and isoquercitroside from aurea helianthus
  • Method for separating and purifying hyperoside and isoquercitroside from aurea helianthus

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Experimental program
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Effect test

Embodiment 1

[0022] A. Accurately weigh 5.0 g of dry sunflower sunflower powder, heat and reflux extract with 70% ethanol solution for 3 hours at a temperature of 80° C., and suction filter after cooling to room temperature to obtain sunflower sunflower residue and supernatant, Collect the supernatant, and dilute it with 70% ethanol solution to a 250mL volumetric flask to obtain the sample solution;

[0023] B. Measure 10 mL of goldenflower sunflower sample solution, adjust the pH value to 10.5 with potassium hydroxide solution, add a certain mass concentration of zinc sulfate, and add an amount to ensure that the mass ratio of goldenflower sunflower sample to zinc sulfate is 1 / 1. After the addition, the complexation reaction time was 3h. The temperature of the complexation reaction is 30°C, centrifuged to obtain the supernatant and the zinc salt-flavone complex, and the supernatant is collected;

[0024] C. The above-mentioned supernatant was heated and concentrated to obtain high-purity...

Embodiment 2

[0026] A. Accurately weigh 5.0 g of dry sunflower sunflower powder, heat and reflux extract with 70% ethanol solution for 3 hours at a temperature of 80° C., and suction filter after cooling to room temperature to obtain sunflower sunflower residue and supernatant, Collect the supernatant, and dilute it with 70% ethanol solution to a 250mL volumetric flask to obtain the sample solution;

[0027] B. Measure 10 mL of goldenflower sunflower sample solution, adjust the pH value to 12.5 with sodium carbonate solution, add a certain mass concentration of zinc chloride, and add an amount to ensure that the mass ratio of goldenflower sunflower sample to zinc chloride is 1 / 0.5. After the addition, the complexation reaction time was 12h. The temperature of the complexation reaction is 40°C, centrifuged to obtain the supernatant and the zinc salt-flavone complex, and the supernatant is collected;

[0028] C. The above supernatant was heated and concentrated to obtain high-purity hyperin...

Embodiment 3

[0030] A. Accurately weigh 5.0 g of dry sunflower sunflower powder, heat and reflux extract with 70% ethanol solution for 3 hours at a temperature of 80° C., and suction filter after cooling to room temperature to obtain sunflower sunflower residue and supernatant, Collect the supernatant, and dilute it with 70% ethanol solution to a 250mL volumetric flask to obtain the sample solution;

[0031] B. Measure 10 mL of goldenflower sunflower sample solution, adjust the pH value to 11.5 with sodium hydroxide solution, add a certain mass concentration of zinc acetate, and add an amount to ensure that the mass ratio of goldenflower sunflower sample to zinc acetate is 1 / 5. After the addition, the complexation reaction time was 1 h. The temperature of the complexation reaction is 10°C, centrifuged to obtain the supernatant and the zinc salt-flavone complex, and the supernatant is collected;

[0032] C. The above supernatant was heated and concentrated to obtain high-purity hyperin and...

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Abstract

The invention relates to a method for separating and purifying hyperoside and isoquercitroside from aurea helianthus, belonging to the field of extraction and separation of natural compounds. The method comprises the following steps: heating, refluxing, and extracting flavones in the aurea helianthus; under alkaline condition, forming stable complex precipitate by zinc salt with flavonoids exceptthe hyperoside and isoquercitroside in the aurea helianthus; centrifuging to remove the complex precipitate; and concentrating supernatant, thus obtaining high-purity hyperoside and isoquercitroside.The method provided by the invention can be used for rapidly, efficiently and safely separating and purifying the hyperoside and isoquercitroside from the aurea helianthus and is beneficial to realization of industrial production of the hyperoside and isoquercitroside.

Description

technical field [0001] The invention relates to a method for separating and purifying hyperin and isoquercitrin from sunflower. Specifically, starting from the extract of goldenflower sunflower, using the weak complexing ability of hyperin and isoquercitrin with zinc salts, and the strong complexing ability of other flavonoids with zinc salts, the removal of goldenflower sunflower Other flavonoids in the extraction solution, and develop a rapid, efficient and safe method for separating and purifying hyperin and isoquercitrin from annulus annuus. It belongs to the field of natural compound extraction and separation. Background technique [0002] Hyperoside is also known as quercetin-3-O-β-D-galactopyranoside, isoquercitrin is also known as quercetin-3-O-β-D-glucopyranoside, both It is the glycoside of quercetin. It has the same aglycon and similar polarity. Only the configuration of a hydroxyl group in the sugar moiety is different. The former is galactoside, the latter is ...

Claims

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Application Information

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IPC IPC(8): C07H17/07C07H1/08
CPCC07B2200/07C07H1/08C07H17/07
Inventor 孙立权张晓娇
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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