Method for separating rutin, hyperoside, isoquercitrin and quercetin from lotus leaves

A technology of hyperoside and isoquercitrin, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve problems such as difficult and effective separation, unsuitable separation, and low separation efficiency

Inactive Publication Date: 2015-04-15
湖州市食品药品检验所
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There is a certain amount of isoquercitrin in lotus leaf, but due to the similar structure of isoquercitrin and flavonoids such as rutin and quercetin, it is difficult to effectively separate it by conventional methods
[0005] CN100367976 (2008-2-13) provided an extract of Hypericum perforatum and its preparation method, which involves the peak area of ​​isoquercitrin and the fingerprint detection method of Hypericum perforatum extract, but this method is not suitable for separating and Not applicable
[0006] "Pharmaceutical Analysis Impurities" 2010, 30 Yuan Lichun, Liu Bin, Shi Renbing disclosed a "HPLC method to determine the content of hyperoside and isoquercitrin in different commercially available lotus leaf medicinal materials", but this method is for routine analysis and purification method, the separation efficiency is not high, and it is difficult to separate quercetin, rutin, hyperoside and isoquercitrin

Method used

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  • Method for separating rutin, hyperoside, isoquercitrin and quercetin from lotus leaves
  • Method for separating rutin, hyperoside, isoquercitrin and quercetin from lotus leaves
  • Method for separating rutin, hyperoside, isoquercitrin and quercetin from lotus leaves

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1 Instruments and reagents

[0027] Agilent 1100 high performance liquid chromatography; Isoquercitrin reference substance (batch number 10391, purity 93.0%, German HWI ANALYTIK company); quercetin reference substance (batch number 100081-200406, China Institute for the Control of Pharmaceutical and Biological Products) ; Rutin reference substance (batch number is 100080-200707, National Institute for the Control of Pharmaceutical and Biological Products); Acetonitrile (chromatographically pure, German Merck company); dried, crushed, and passed through a No. 5 sieve); the rest of the reagents are analytically pure.

[0028] 2 Methods and results

[0029] 2.1 Chromatographic conditions

[0030] Chromatographic column: Agilent C18 column (250mm×4.6mm, 5μm) with a column temperature of 30°C. Mobile phase A: pH4.0 phosphate buffer (0.2% phosphoric acid solution, adjust the pH value to 4.0 with 10% sodium hydroxide solution); mobile phase B: acetonitrile; see Table 1 for ...

Embodiment 2

[0054] The same as in Example 1, the difference is that the volume ratio of mobile phase A to mobile phase B, that is, the gradient elution conditions, is shown in Table 3 at different times. The phosphate buffer solution is prepared by mixing 0.15% phosphoric acid solution and 8% sodium hydroxide solution to a pH value of 3.5. The lotus leaf powder was first dissolved with 75% methanol solution and ultrasonicated for 28 minutes, then dissolved with 85% methanol solution and filtered, and the filtrate was used as the separation liquid for the column chromatography.

[0055] The measured yield of rutin was 96.5% with a purity of 90.5%; the yield of hyperoside was 97.3% with a purity of 89%; the yield of isoquercitrin was 99% with a purity of 92.3%; the yield of quercetin It is 98.4%, and the purity reaches 90.4%.

[0056] Table 3 Example two gradient elution table

[0057]

Embodiment 3

[0059] The same as in Example 1, the difference is that the volume ratio of mobile phase A to mobile phase B, that is, the gradient elution conditions, is shown in Table 4 at different times.

[0060] The measured yield of rutin was 96.2% with a purity of 89.9%; the yield of hyperoside was 97.1% with a purity of 89.4%; the yield of isoquercitrin was 98.9% with a purity of 91.8%; the yield of quercetin It is 97.8%, and the purity reaches 90.1%.

[0061] Table 4 Example three gradient elution table

[0062]

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Abstract

The invention relates to the technical field of traditional Chinese medicine, especially to a method for separating rutin, hyperoside, isoquercitrin and quercetin from lotus leaves. The method for separating the rutin, the hyperoside, the isoquercitrin and the quercetin from the lotus leaf comprises separating components in the lotus leaves powder by using a column chromatography method for a gradient elution for 65 to 75 minutes with a flow rate of 0.6 to 0.8 ml / min under a temperature of 28 to 32 DEG C and a pH value of 3.5 to 4.5, wherein a stationary phase of the column chromatography is C18, a mobile phase A is a phosphatic buffer with a pH value of 3.5 to 4.5, a mobile phase B is acetonitrile, and the outflow components are successively the rutin, the hyperoside, the isoquercitrin and the quercetin according to a time sequence; respectively recovering eluents containing the rutin, the hyperoside, the isoquercitrin and the quercetin; and finally removing solvents with reduced pressure distillation to obtain the rutin, the hyperoside, the isoquercitrin and the quercetin. The method not only can separate the rutin, the hyperoside, the isoquercitrin and the quercetin, but also is high is separation efficiency.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicines, in particular to a method for separating rutin, hyperin, isoquercitrin and quercetin from lotus leaves. Background technique [0002] Lotus leaf is the dry leaf of water lily plant lotus (Nelumbo nucifera Gaertn), also known as lotus leaf and lotus leaf. It is widely planted in most parts of my country and widely used in food and medicine. Drugs again". According to traditional medicine, the lotus leaf tastes bitter and flat, and it returns to the liver, spleen, and stomach meridian. Thin and poor", "Grow vitality, help the spleen and stomach, astringent essence and turbidity, dispel blood stasis, reduce swelling and pain, and cause acne". Modern medical research has proved that lotus leaves contain a large amount of flavonoids, which have the effects of lowering blood fat, treating atherosclerosis, anti-mitosis, and inhibiting bacteria. [0003] At present, the determinat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/07C07H1/08C07D311/30C07D311/40
Inventor 王新财陈晓平
Owner 湖州市食品药品检验所
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