Application of alpha-L-rhamnoside enzyme in directional synthesis of isoquercitrin by biological conversion of rutin

Abstract

The invention relates to application of an alpha-L-rhamnoside enzyme in the directional synthesis of isoquercitrin by the biological conversion of rutin. Because the crude enzyme preparation or the immobilized enzyme preparation of the alpha-L-rhamnoside enzyme is used for catalyzing and hydrolyzing the rutin and directionally and biologically synthesizing the isoquercitrin, the application has the advantages of wide source, easy preparation and low cost of catalysts, high stability, and high catalysis efficiency and specificity of the enzyme preparation, and is easy to store. Thus, the alpha-L-rhamnoside enzyme can greatly reduce the production cost of quercetin, the catalysis and conversion rate of the enzymes is high, and the products are single.

Description

1. Technical field

[0001] The invention belongs to the field of natural product biosynthesis, and in particular relates to a method for directional synthesis of isoquercitrin through enzymatic biotransformation of rutin. 2. Background technology

[0002] Existing technology: Isoquercitrin (Quercetin-3-glucose) is a secondary glycoside produced by the hydrolysis of α-L-rhamnosyl in rutin molecule, which has anti-oxidation, blood pressure-lowering, anti-inflammatory and anti-cancer properties. It has similar or even higher bioactivity and bioavailability to rutin and quercetin in terms of inhibiting platelet aggregation and other aspects, and plays an important role in delaying human aging and preventing and curing diseases. The results of relevant pharmacological experiments show that isoquercitrin has the effects of promoting body fluid and quenching thirst, clearing heat and relieving heat, lowering blood pressure, strengthening the heart and prolonging life, and can also b...

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