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Maniod ebish flower extract, extraction and analysis method and extract preparation and use thereof

A technology of hollyhock flower and extraction method, which is applied in the directions of medical preparations containing active ingredients, plant/algae/fungus/moss ingredients, and pill delivery, etc. and other problems, to achieve a reliable curative effect and a significant effect on the treatment of cardiovascular and cerebral ischemic diseases

Inactive Publication Date: 2009-03-18
周春晓 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of this technical solution are: one, because the hexahydroxyflavone myricetin, gossetin and their glycosides are unstable in 30% ethanol; its content
Pass the filtrate after extraction and separation through weak polarity and medium polarity macroporous adsorption resins such as AB-8 and DM301, or polyamide column chromatography to enrich total flavonoids. Although polyamide column chromatography can obtain high-purity total flavonoids, Because the polyamide column needs alkali-acid regeneration after each chromatography, this method has high cost and long working hours, and is not suitable for the preparation of second-class raw materials for oral use
[0014] The total flavonoids of marshmallow flowers and its preparations have only been reported in clinical pharmacology for the treatment of oral ulcers

Method used

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  • Maniod ebish flower extract, extraction and analysis method and extract preparation and use thereof
  • Maniod ebish flower extract, extraction and analysis method and extract preparation and use thereof
  • Maniod ebish flower extract, extraction and analysis method and extract preparation and use thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0044] Take 1kg of hollyhock peanut medicine powder, add 10000ml of 75% ethanol and heat reflux for extraction for 2 hours, extract 3 times in total, combine the extracts, let cool, filter, recover the ethanol under reduced pressure to 1 / 5 volume, stand for 12 hours and filter; Through HPD400 macroporous adsorption resin column chromatography, first wash 5-8 column volumes with water, eluting 1-2 column volumes with 10% ethanol, and then eluting with 65% ethanol until there is no AlCl 3 For the reaction, collect the 65% ethanol eluate; concentrate the eluate under reduced pressure at a temperature lower than 60°C and vacuum dry to obtain the crude extract of hollyhock flower; take the crude extract and use 10 times the amount (W / V) The boiling point ethyl acetate-ethanol refluxed extraction 3 times, each 1-2 hours, the solvent was recovered, and dried under reduced pressure at 80°C to obtain the hollyhock flower extract product. The yield of the extract is 3.5% by weight, and the ...

Embodiment 2

[0046] Take 1kg of hollyhock peanut medicine powder, add 10000ml of 75% ethanol and heat reflux for extraction for 2 hours, extract 3 times in total, combine the extracts, let cool, filter, recover ethanol to 1 / 5 volume under reduced pressure, stand for 12 hours and filter; HPD700 macroporous adsorption resin column chromatography, first rinse with water for 5-8 column volumes, eluted with 10% ethanol for 1-2 column volumes, and then with 65% ethanol or eluted to no AlCl 3 For the reaction, collect 65% ethanol or the eluent; concentrate the eluent under reduced pressure at a temperature lower than 60°C and vacuum dry to obtain the crude extract of hollyhock flower; take the crude extract and use 10 times the amount (W / V) Azeotropic ethyl acetate-methanol reflux extraction 3 times, 1-2 hours each time, the solvent was recovered, and dried under reduced pressure at 60°C to obtain the hollyhock flower extract product. The yield of the extract is 3.7% by weight. The total flavonoids (...

Embodiment 3

[0048] Take 1kg of hollyhock peanut medicine crude powder, add 8000ml of 60% ethanol and heat reflux for extraction for 2 hours, extract 3 times, combine the extracts, let cool, filter, filtrate, and concentrate the filtrate under reduced pressure under 60℃ to concentrate The ratio of volume to crude drug weight is 0.5-1:1. Use 1:1 (V / V) water-saturated n-butanol to draw 8-10 times, combine the n-butanol extracts, and dry with anhydrous sodium sulfate. Filter, concentrate under reduced pressure at less than 80℃, and dry in vacuum to obtain crude extract of Abelmoschus manihot; take the crude extract and extract 3 times with 10 times (W / V) azeotropic ethyl acetate-ethanol reflux, each time After 1-2 hours, the solvent is recovered, and dried under reduced pressure at 80° C. to obtain the hollyhock flower extract product. The yield of the extract is 3.2% by weight, and its total flavonoids (calculated as hyperoside) content is 56.0%. The content of the following flavonoids is: hyper...

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Abstract

An extract from Sunset Abelmoschus, containing 50-75wt% of total flavone counted by hyperin, wherein, the contents of hyperin, isoquercitrin and quercetin-3'-glucoside are 10.0-15.0wt%, 7.0-12.0wt% and 8.0-12.0wt% respectively, and a total flavone finger-print is provided. The extract is obtained by the following steps: a concentrated solution of a Sunset Abelmoschus ethanol extract undergoes chromatography through an HPD400 or HPD700 macroporous absorbent resin column to derive a crude extract or is extracted by normal butanol to derive the crude extract; a refined extract is obtained by refluxing extraction with ethyl acetate-ethanol azeotropic mixture or ethyl acetate-methanol mixture; the refined extract goes through pressure reducing exsolution and drying. Corresponding contents and a method for detecting finger-print are also provided. The extract preparation is a dripping pill, tablet, capsule or soft capsule obtained through processing with the extract as an active ingredient and pharmaceutical excipients. The extract can be used for preparing a medicine preparation for treating cardiac anencephalohemia diseases.

Description

1. Technical Field [0001] The present invention relates to active ingredients in Chinese herbal medicines, extraction and analysis methods, and preparations and uses of the extracts, in particular, hollyhock flower extracts, extraction and analysis methods, and extract preparations and uses. 2. Background technology [0002] The hollyhock flower is the dried flower of Abelmoschus Manihot (L.) Medic, a plant of the genus Abelmoschus in the Malvaceae family. The medicinal material is included in the Supplement to the Standard of Chinese Materia Medica of Jiangsu Province (1989 edition). [0003] Regarding the chemical composition of the hollyhock flower, Wang Xianrong et al. in 1981, in Acta Botany, 23(3): 222-226 (1981), reported "Study on the chemical composition of the hollyhock flower". Quercetin-3 was isolated from the hollyhock flower. -5 kinds of flavonoids such as lobe glycoside, hyperoside, quercetin-3'-glucoside, myricetin and quercetin. In 2004, Wang Xianrong and others ...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61K9/20A61K9/48A61P9/10A61K133/00
Inventor 王勤周春晓陈志武袁慧汪年生赵磊
Owner 周春晓
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