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Method for Determination of Chlorogenic Acid, Caffeic Acid and Luteolin Contents in C.

A technology of luteolin and chlorogenic acid, which is used in measuring devices, instruments, scientific instruments and other directions to achieve the effects of saving resource loss, accurate measurement, comprehensive and effective evaluation and control

Active Publication Date: 2021-09-21
GUANGXI UNIV OF CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few relevant researches on the quality analysis of chrysanthemum, after reviewing the literature, there is no report on the determination of chlorogenic acid, caffeic acid and luteolin in chrysanthemum at home and abroad.

Method used

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  • Method for Determination of Chlorogenic Acid, Caffeic Acid and Luteolin Contents in C.
  • Method for Determination of Chlorogenic Acid, Caffeic Acid and Luteolin Contents in C.
  • Method for Determination of Chlorogenic Acid, Caffeic Acid and Luteolin Contents in C.

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Embodiment 1

[0024] Using the above-mentioned method of one test and multiple evaluations to determine the content of chlorogenic acid, caffeic acid and luteolin in C. chinensis, 10 batches of C. cicadae were tested. The specific determinations are as follows:

[0025] 1 Experimental materials, instruments and reagents

[0026] 1.1 Experimental materials

[0027] See Table 2 for the harvesting area and date of the Chinese herbal medicines, and it was identified by Professor Wei Songji of Guangxi University of Traditional Chinese Medicine as a plant of the genus A. Ixeridium chinense (Thunb.) Tzvel.) Whole plant.

[0028]

[0029] 1.2 Instruments

[0030] U3000 high-performance liquid chromatography (Thermo Fisher, USA); 1260 high-performance liquid chromatography (Agilent, USA); column CAPCELLPAK TYPEⅡSIZE C18 (4.6mmI.D.×250mm, 5µm), AgilentEclipse plus C18 (4.6×250mm, 5µm), Waters Atlantis T3 (4.6×250mm, 5µm); XS-205Du electronic analytical balance (Switzerland METTLER TOLEDO company)...

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Abstract

The present invention relates to the technical field of detection of traditional Chinese medicine components, in particular to a method for determining the content of chlorogenic acid, caffeic acid and luteolin in C. chinensis by using the method of one test and multiple evaluations, comprising the following steps: (1) Preparation of a reference solution (2) Preparation of the test solution (3) Chromatographic conditions and system suitability test (4) Determination. The present invention uses chlorogenic acid as an internal reference to carry out one test and multiple evaluations, establishes a relative correction factor between chlorogenic acid, caffeic acid and luteolin, and calculates chlorogenic acid, caffeic acid and luteolin in C. Grass glycoside content. The method of the present invention is simple, easy to operate, high in practicability, and accurate in results. The method can realize the synchronous determination of the content of chlorogenic acid, caffeic acid and luteolin in A. chinensis, thereby effectively evaluating and controlling the content of the medicinal materials of A. chinensis. Quality, to provide a scientific basis for ensuring the safety and effectiveness of clinical medication and the development of medicinal material resources.

Description

technical field [0001] The invention relates to the technical field of detection of traditional Chinese medicine components, in particular to a method for measuring the contents of chlorogenic acid, caffeic acid and luteolin in chrysanthemum by adopting a one-test and multiple-evaluation method. Background technique [0002] Mountain bittern ( Ixeridium chinense (Thunb.) Tzvel.) is a perennial herbaceous plant of the family Asteraceae. The whole herb is used as medicine. It is also known as Chinese bitter vegetable, mountain bitter vegetable, small bitter vegetable, etc. It is distributed in northern, southern and eastern provinces and regions. It can be used as medicine It is edible and edible. It has the effects of clearing heat and detoxifying, reducing inflammation and cooling blood, relieving pain and swelling, and anti-tumor. It is used to treat unknown swelling and pain, abdominal abscess, dysentery, appendicitis, pneumonia, arthritis and other diseases. [0003] Liu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/86
CPCG01N30/8679G01N30/88G01N2030/8809
Inventor 梁爽朱华赵立春黄健军卢森华陈洁银吴秀彩
Owner GUANGXI UNIV OF CHINESE MEDICINE
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