A method for purifying schisandrin A and schisandrin B from schisandra extract

A technology of schisandra extract and schisandra alcohol A, which is applied in ether separation/purification, organic chemistry, ether preparation, etc., can solve the problems of high cost, complicated purification method operation, and easy residual toxic and harmful substances, and achieve low cost and good quality Prospect of medicine and health food, effect of reducing dosage

Active Publication Date: 2016-07-06
劲牌持正堂药业有限公司
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0005] The purpose of the present invention is to solve the current purification methods of schisandrin A and schisandrin B with complex operation, high cost, and easy residual toxic and harmful substances, and provide a simple operation, safe and non-toxic, low cost, high recovery rate, And the method that can simultaneously purify schisandrin A and schisandrin B has good prospects for the development of medicine and health food

Method used

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  • A method for purifying schisandrin A and schisandrin B from schisandra extract
  • A method for purifying schisandrin A and schisandrin B from schisandra extract
  • A method for purifying schisandrin A and schisandrin B from schisandra extract

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Embodiment 1

[0026] A method for purifying schisandrin A and schisandrin B from Schisandra chinensis extract of the present embodiment comprises the following steps:

[0027] (1) Extraction: Add 800L of pure water to 100Kg of schisandra crude drug, reflux and extract at 70°C for 2 hours, collect the dregs by filtration, and discard the filtrate; add 800L of 90% (v / v) ethanol aqueous solution to the dregs each time , reflux extraction at 80°C for 2 times, each time for 2h, collect each extract and detect the content of schisandrin A and schisandrin B, the detection spectrum is shown in image 3 , figure 2 It is the spectrogram of the standard substance of schisandrin A and schisandrin B;

[0028] (2) Concentration: Concentrate the extract to 50L, then add 300L of pure water to the concentrated solution, cool it at 0-4°C and let it stand for 5 hours for water sinking, and perform the first centrifugation of the water sinking treatment liquid, The centrifugal speed is 8000r / m, and the prec...

Embodiment 2

[0034] see figure 1 , the present invention comprises the following steps:

[0035](1) Extraction: Add 500L of 95% (v / v) ethanol aqueous solution to 100Kg Schisandra crude drug each time, reflux extraction at 90°C for 4 times, each time for 2 hours, and collect the extraction solution each time;

[0036] (2) Concentration: Concentrate the extract to 80L, then add 400L of pure water to the concentrated solution, cool it at 0-4°C and let it stand for 4 hours for water sinking, and perform the first centrifugation of the water sinking treatment liquid, The centrifugal speed is 3000r / m, and the precipitate and supernatant are obtained after centrifugation. 90-95% of schisandrin and 50%-55% of schisandrin enter into the precipitate, 5-10% of schisandrin and 45-50% The schisandra alcohol A stays in the supernatant, and the precipitation is to be processed;

[0037] (3) Adsorption: Add 10Kg of activated carbon to the supernatant for adsorption, stir and absorb for 2 hours, then per...

Embodiment 3

[0041] see figure 1 , the present invention comprises the following steps:

[0042] (1) Extraction: Add 1000L of 70% (v / v) ethanol aqueous solution to 100Kg Schisandra crude drug each time, reflux extraction at 50°C for 2 times, each time for 3 hours, and collect the extraction solution each time;

[0043] (2) Concentration: Concentrate the extract to 30L, then add 200L of pure water to the concentrated solution, cool it at 0-4°C and let it stand for 6 hours for water sinking, and perform the first centrifugation of the water sinking treatment liquid, The centrifugal speed is 10000r / m. After centrifugation, the precipitate and supernatant are obtained. 90-95% of Schizandrin B and 50%-55% of Schizandrin A enter the precipitate, 5-10% of Schisandrin B and 45-50% The schisandra alcohol A stays in the supernatant, and the precipitation is to be processed;

[0044] (3) Adsorption: Add 20Kg of activated carbon to the supernatant for adsorption, stir and absorb for 3 hours, then pe...

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Abstract

A method for purifying Schisandrin A and Schisandrin B from the Schisandra chinensis extract, which comprises the following steps: after the crude drug of Schisandra chinensis is extracted, collecting the extract, concentrating the extract to 0.3-0.8 times the weight of the crude drug, and adding a volume of Pure water 2-4 times the weight of the crude drug, cool and stand for 4-6 hours at 0-4°C for water sedimentation, perform the first high-speed centrifugation, and precipitate for treatment; add 10-20% of the crude drug weight to the supernatant After the activated carbon was stirred and adsorbed for 0.5-3 hours, the second high-speed centrifugation was performed, and the centrifugation supernatant was discarded; 4-20 times the weight of the crude drug was added to the activated carbon with 60-95% (v / v) ethanol solution, and heated to 50 Elute at -80°C for 1-3 hours, and collect the supernatant after the third high-speed centrifugation; add the supernatant to the precipitate after the first centrifugation, stir, and add 3-8% of the crude drug weight after homogenization. After drying, the dry extract containing schisandrin A and B can be obtained; the method has the advantages of simple operation, safety and non-toxicity, low cost and high recovery rate, can simultaneously recover schisandrin A and schisandrin B, and has good medicinal properties. and health food development prospects.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine preparation, in particular to a method for purifying schisandrin A and schisandrin B from schisandra extract. Background technique [0002] Schisandra chinensis (Turcz.) Baill., a traditional Chinese medicine, is the dry and mature fruit of Schisandrachinensis (Turcz.) Baill. It has the effects of astringent and astringent, nourishing qi and promoting body fluid, invigorating the kidney and calming the heart. It is often used for chronic cough and shortness of breath, nocturnal emission, enuresis, frequent urination, chronic diarrhea, spontaneous sweating, thirsty wounds, internal heat and quenching thirst, palpitations and insomnia. [0003] Schisandra contains schisandrin A, schisandrin A, schisandrin B, schisandra C and other lignans, which have good liver protection and enzyme-lowering effects, and are often used in traditional Chinese medicine and health food, such as Su...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C43/23C07C41/34C07D317/70
CPCC07C41/34C07C2603/36C07D317/70C07C43/23
Inventor 张雪元周彬杨跃军汪陈平吴建辉杨波
Owner 劲牌持正堂药业有限公司
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