Medium for inducing differentiation of human amniotic epithelial stem cells into melanocytes and method thereof

Abstract

The invention relates to a medium for inducing differentiation of human amniotic epithelial stem cells into melanocytes and a method thereof. Endothelin 3, dexamethasone, stem cell factor, basic fibroblast growth factor, insulin transferrin and fetal calf serum were added to α‑MEM basal medium. The method is to make human amniotic membrane into a suspension of human amniotic epithelial stem cells; place the cells in α‑MEM supplemented with endothelin 3, dexamethasone, stem cell factor, basic fibroblast growth factor, insulin transferrin and fetal bovine serum medium; at 37°C, 5% CO 2 Amniotic epithelial stem cells were cultured into an adherent monolayer in an incubator, and gradually induced to differentiate into cells expressing melanocyte marker genes tyrosinase-related protein 1 (trp1), 2 (trp2), microphthalmia-related transcription factor (mitf), tyrosine acidase (dtc) positive melanocytes. It has the characteristics of rich cell sources, low immunogenicity, no ethical restrictions, and simple induction method.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a culture medium and a method thereof for differentiation of epithelial stem cells into melanocytes, and specifically relates to a culture medium for inducing differentiation of human amniotic epithelial stem cells into melanocytes and a method thereof. Background technique [0002] Skin pigmentation plays a vital role in human health and skin aesthetics, especially in exposed areas such as the face. Since the pigment synthesized by melanocytes can protect the body from environmental attacks and the invasion of various dangerous cells, the lack of skin pigment can not only lead to immune diseases such as vitiligo, but also affect the mental health of patients. [0003] Vitiligo is a common acquired localized or generalized skin depigmentation disease. Due to the loss of melanocyte function in the skin, it can occur in various parts of the body, and it is common in the back of the fing...

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at problems such as difficulty in source of autologous melanocytes and restriction of immunogenicity of allogeneic melanocytes in the existing methods for treating vitiligo, and provides a kind of human amniotic epithelial stem cells that have a wide range of allogeneic sources and no or low immunogenicity. Cell induction differentiation medium and method thereof

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