Preparation method of chitosan quaternary ammonium salt hyaluronic acid nanogel coated with basic fibroblast growth factors

A chitosan quaternary ammonium salt and fibroblast technology, which is applied in the field of pharmacy, can solve the problems of easy degradation and inactivation, limited therapeutic effect, etc., and achieves simple preparation technology, high encapsulation capacity, and good mucoadhesion. Effect

Inactive Publication Date: 2015-09-16
青岛华迈士药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing prominent problems are: 1. Basic fibroblast growth factor is easily degraded and inactivated in the oral environment; 2. With the secretion of human saliva, basic fibroblast growth factor will be quickly cleared on the ulcer surface, limiting its therapeutic effect

Method used

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  • Preparation method of chitosan quaternary ammonium salt hyaluronic acid nanogel coated with basic fibroblast growth factors
  • Preparation method of chitosan quaternary ammonium salt hyaluronic acid nanogel coated with basic fibroblast growth factors

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Take 30 mg of chitosan quaternary ammonium salt with a molecular weight of 40 kDa, dissolve it in 10 mL of deionized water, add 10 mL of 2 mg / mL basic fibroblast growth factor solution dropwise under stirring, and 1 mL of 0.2% (w / v) tripolyphosphoric acid Sodium solution, drop in 20 minutes, stir at 200 rpm at room temperature for 30 minutes, add 10 mL of sodium hyaluronate solution containing 0.3% (w / v) dropwise under stirring conditions, drop in 30 minutes, adjust with sodium hydroxide pH to pH 4.0, stirred at room temperature, stirred at 200 rpm for 5 hours, centrifuged at 15,000 rpm for 2 hours, removed the supernatant, and freeze-dried to obtain the chitosan quaternary ammonium hyaluronic acid loaded with basic fibroblast growth factor acid nanogel.

Embodiment 2

[0026] Take 30 mg of chitosan quaternary ammonium salt with a molecular weight of 90 kDa, dissolve it in 10 mL of deionized water, add 10 mL of 2 mg / mL basic fibroblast growth factor solution dropwise under stirring, and 1 mL of 0.3% (w / v) tripolyphosphoric acid Sodium solution, drop in 20 minutes, stir at 200 rpm at room temperature for 30 minutes, add 10 mL of sodium hyaluronate solution containing 0.4% (w / v) dropwise under stirring conditions, drop in 30 minutes, adjust with sodium hydroxide pH to pH 5.0, stirred at room temperature, stirred at 200 rpm for 5 hours, centrifuged at 15,000 rpm for 2 hours, removed the supernatant, and freeze-dried to obtain the chitosan quaternary ammonium hyaluronic acid loaded with basic fibroblast growth factor acid nanogel.

Embodiment 3

[0028] Take 30 mg of chitosan quaternary ammonium salt with a molecular weight of 150 kDa, dissolve it in 10 mL of deionized water, add 10 mL of 2 mg / mL basic fibroblast growth factor solution dropwise under stirring, and 1 mL of 0.5% (w / v) tripolyphosphoric acid Sodium solution, drop in 20 minutes, stir at 200 rpm at room temperature for 30 minutes, add 10 mL of sodium hyaluronate solution containing 0.5% (w / v) dropwise under stirring conditions, drop in 30 minutes, adjust with sodium hydroxide pH to pH 6.0, stirred at room temperature, stirred at 200 rpm for 5 hours, centrifuged at 15000 rpm for 2 hours, removed the supernatant, and freeze-dried to obtain the chitosan quaternary ammonium hyaluronic acid loaded with basic fibroblast growth factor acid nanogel.

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Abstract

The invention discloses a preparation method of chitosan quaternary ammonium salt hyaluronic acid nanogel coated with basic fibroblast growth factors. The preparation method of chitosan quaternary ammonium salt hyaluronic acid nanogel coated with basic fibroblast growth factors is characterized by taking chitosan quaternary ammonium salt and sodium hyaluronate as raw materials and synthesizing a target product through a two-step process of ionic cross-linking and polymer condensation. The preparation method comprises the following steps: firstly mixing a chitosan quaternary ammonium salt solution with a basic fibroblast growth factor solution, and then dropwise adding a sodium tripolyphosphate solution into the mixed solution to prepare an ionic cross-linked mixed solution; and finally dropping sodium hyaluronate into the ionic cross-linked mixed solution for carrying out polymer condensation to prepare chitosan quaternary ammonium salt hyaluronic acid nanogel coated with basic fibroblast growth factors. The average particle diameter of the nanogel is 150-230nm; the Zeta potential is -28.6 to -36.7mV. The nanogel can be used for treating dental ulcer and has good research, development and utilization prospects.

Description

technical field [0001] The invention belongs to the field of pharmacy and relates to a preparation technology of nano gel sustained-release particles, in particular to a preparation method of chitosan quaternary ammonium salt hyaluronic acid nano gel loaded with basic fibroblast growth factor. Background technique [0002] Oral ulcer is a common disease in daily life, mostly occurs on the tip of the tongue, belly of the tongue, and buccal mucosa, accompanied by intermittent, tactile tingling, which seriously affects the quality of life of patients. At present, there is still no effective method for the treatment of oral ulcers. The traditional treatment method focuses on local pain relief, but does not pay enough attention to how to speed up the healing of the ulcer surface. Basic fibroblast growth factor is a multifunctional protein bioactive substance, which has a strong repair effect on wound surface and nerve injury. The use of basic fibroblast growth factor to treat or...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K38/18A61K9/19A61K47/36A61P1/02
Inventor 徐全臣王志国许晓燕袁昌青吉秋霞
Owner 青岛华迈士药业有限公司
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