Human stem cell factor skin repairing solution and preparation method thereof

A stem cell factor and skin repair technology, applied in the field of damaged skin repair, can solve the problems of inconspicuous effect, single dosage and concentration, difference, etc., to achieve the effect of improving the quality of life, restoring skin quality, and uncomplicated process

Active Publication Date: 2015-05-20
姜振宇
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Now there are some cytokine drugs and cosmetics on the market, most of which are single cytokines, such as epidermal growth factor (EGF), basic fibroblast growth factor (BFGF), etc., a

Method used

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  • Human stem cell factor skin repairing solution and preparation method thereof
  • Human stem cell factor skin repairing solution and preparation method thereof
  • Human stem cell factor skin repairing solution and preparation method thereof

Examples

Experimental program
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preparation example Construction

[0026] The preparation method of the above-mentioned human stem cell factor skin repair solution comprises the following steps:

[0027] (1) Aseptically collect qualified newborn umbilical cords, and adopt tissue block direct culture method to cultivate;

[0028] (2) After discarding the tissue pieces, replace with fresh serum-free medium to continue culturing;

[0029] (3) After the cells were 90% confluent, the supernatant was collected, digested with trypsin, and centrifuged to obtain cells;

[0030] (4) Inoculate the cells in culture dishes / bottles for culture, add serum-free medium to continue passage;

[0031] (5) The collected cell culture fluid is centrifuged and filtered through a 0.2um aperture filter;

[0032] (6) The supernatant was screened with molecular weight and diameter as indicators.

[0033] (7) Add cytokine formula, mix and store.

[0034] The product obtained in the first six steps of this preparation method will filter out other factors greater than ...

Embodiment 1

[0043] A kind of human stem cell factor skin repair liquid, is made of the composition of following content:

[0044]

[0045] The specific operation of the preparation method is as follows:

[0046] 1. Operating procedures for umbilical cord collection

[0047] 1 Before the umbilical cord is collected, the collection staff must strictly check the name, age, and ID information of the parturient, whether the parturient has hepatitis B, hepatitis C, AIDS, and syphilis infection, and those who meet the preservation standards can collect the umbilical cord.

[0048] 2 Umbilical cord collection bottles must be sterile containers approved for human tissue collection to reduce the risk of cell loss and microbial contamination.

[0049] 3 Check whether the collection bottle is complete, airtight, and kept sterile, and whether the outer packaging is damaged. If it is damaged, it cannot be used.

[0050] 4. All reagents and materials used to collect the umbilical cord must be steri...

Embodiment 2

[0133] A kind of human stem cell factor skin repair liquid, is made of the composition of following content:

[0134]

[0135] The specific operation of the preparation method is as in Example 1.

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Abstract

The invention discloses a human stem cell factor skin repairing solution and a preparation method thereof. The solution is prepared from the following components by content: 50-75ug/l of epidermal growth factor, 15-20ug/l of acidic fibroblast growth factor, 15-20ug/l of basic fibroblast growth factor and 4-6ug/l of vascular endothelial growth factor. A series of cell factors against quick proliferation of germinal cells, and the effects of quickly repairing damaged skin and repairing skin quality can be achieved according to reasonable environment which accords with human cell growth and accelerating growth.

Description

technical field [0001] The invention relates to the technical field of damaged skin repair, in particular to a human stem cell factor skin repair solution and a preparation method thereof. Background technique [0002] Human skin cells are under the stimulation and influence of the external environment for a long time. With the increase of age, skin cells show a tendency of aging. Germinal cells in the basal layer of the skin, that is, cells with the potential to proliferate and expand, are important cells for maintaining skin color, smoothness, texture, and elasticity. However, when encountering some large damage or persistent damage, the proliferative power of germinal cells is not enough to maintain the original skin quality, and the phenomenon of skin aging and degeneration appears. [0003] Now there are some cytokine drugs and cosmetics on the market, most of which are single cytokines, such as epidermal growth factor (EGF), basic fibroblast growth factor (BFGF), etc....

Claims

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Application Information

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IPC IPC(8): A61K8/64A61K8/98A61Q19/00A61K38/18A61K35/51A61Q17/00A61Q17/02
Inventor 张海英阎晓然姜振宇
Owner 姜振宇
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