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Biological agent for repairing skin injury and preparation method of biological agent

A technology for repairing skin damage and biological agents, applied in the field of biological agents for repairing skin damage and its preparation, can solve the problems of slow skin repair, slow repair time, and long treatment period, so as to promote repair and repair quickly , the effect of promoting proliferation

Pending Publication Date: 2019-06-28
四川驰鼎盛通生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the skin is in direct contact with the outside world, the skin is also the most vulnerable to damage. Generally, the skin will repair itself slowly for small injuries, but the repair time is very slow. 2 When the skin is damaged in a large area, it is more difficult to repair through the skin itself Requires external paramedical repair
[0003] At present, the common skin repair method is to repair skin damage through biological artificial skin transplantation, but the biological artificial skin has different immunogenicity from the original patient's skin, which makes the treatment of transplanted skin have great limitations
[0004] In addition, the current skin repair method also uses traditional Chinese medicine for slow conditioning, so that the skin can slowly repair itself, but the treatment cycle is long, and the patient needs to endure the pain for a long time

Method used

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  • Biological agent for repairing skin injury and preparation method of biological agent
  • Biological agent for repairing skin injury and preparation method of biological agent

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Experimental program
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Effect test

Embodiment 1

[0029] This embodiment provides a biological preparation for repairing skin damage, including the following components by weight: 8 g of active fat stem cells, 4 g of amnion, 0.05 g of epidermal growth factor, 0.05 g of fibroblast growth factor, fibroblast 4g, fibronectin 0.005g, deionized water 40ml.

[0030] This embodiment also provides a method for preparing a biological preparation for repairing skin damage, which specifically includes the following steps:

[0031] S1. Collect adipose tissue, cut and pulverize the adipose tissue with scissors or a cutting machine, and pulverize it into 1mm blocks, then add 100ml of normal saline, and shake and wash for 1min on an oscillating shaker with a speed of 1200r / min to remove residual blood stains. After standing still for 10 minutes, after the adipose tissue precipitated, remove the supernatant, repeat washing twice, put the remaining sediment into 100ml collagenase solution for digestion, keep constant temperature at 37°C and co...

Embodiment 2

[0035] This embodiment provides a biological preparation for repairing skin damage, including the following components by weight: 10 g of active fat stem cells, 5 g of amnion, 0.06 g of epidermal growth factor, 0.06 g of fibroblast growth factor, fibroblast 6g, fibronectin 0.008g, deionized water 50ml.

[0036] This embodiment also provides a method for preparing a biological preparation for repairing skin damage, which specifically includes the following steps:

[0037] S1. Collect adipose tissue, cut and pulverize the adipose tissue with scissors or a cutting machine, and pulverize it into 1mm pieces, then add 100ml of normal saline, and shake and wash for 2 minutes on an oscillating shaker with a rotating speed of 1200r / min to remove residual blood stains. After standing still for 15 minutes, after the adipose tissue precipitated, remove the supernatant, repeat washing twice, put the remaining sediment into 100ml collagenase solution for digestion, keep constant temperature...

Embodiment 3

[0041]This embodiment provides a biological preparation for repairing skin damage, including the following components by weight: 12g of active fat stem cells, 6g of amnion, 0.1g of epidermal growth factor, 0.1g of fibroblast growth factor, fibroblast 8g, fibronectin 0.01g, deionized water 60ml.

[0042] This embodiment also provides a method for preparing a biological preparation for repairing skin damage, which specifically includes the following steps:

[0043] S1. Collect adipose tissue, cut and crush the adipose tissue with scissors or a cutting machine, and crush it into 2mm pieces, then add 100ml of normal saline, and wash it on an oscillating shaker with a speed of 1200r / min for 5min to remove residual blood stains. After standing still for 20 minutes, after the adipose tissue precipitated, remove the supernatant, repeat washing twice, put the remaining sediment into 100ml collagenase solution for digestion, keep constant temperature at 37°C and continue to shake and st...

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Abstract

The invention discloses a biological agent for repairing skin injury and a preparation method of the biological agent, and belongs to the technical field of biology. The biological agent for repairingthe skin injury is prepared from, by weight, 8-12 g of adipose-derived stem cell active matter, 4-6 g of amnion, 0.05-0.1 g of epidermal growth factors, 0.05-0.1 g of fibroblast growth factors, 4-8 gof fibroblasts, 0.005-0.01 g of fibronectin and 40-60 ml of deionized water. The biological agent for repairing the skin injury has the advantages that the injured skin can be repaired, and the healing efficiency of the skin is improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a biological preparation for repairing skin damage and a preparation method thereof. Background technique [0002] The skin is the organ that the human body directly contacts with the outside world, and is the protective barrier of the human body. Because the skin is in direct contact with the outside world, the skin is also the most vulnerable to damage. Generally, the skin will repair itself slowly for small injuries, but the repair time is very slow. 2 When the skin is damaged in a large area, it is more difficult to repair through the skin itself Requires external paramedical repair. [0003] At present, the common skin repair method is to repair skin damage through biological artificial skin transplantation, but the biological artificial skin has different immunogenicity from the original patient's skin, which makes the treatment of transplanted skin have great limi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/39A61P17/02A61K35/28A61K35/33A61K35/50A61K38/18
Inventor 杨燕刘康肖春宋丽萍张勇刚张娟罗旭梅
Owner 四川驰鼎盛通生物科技有限公司
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