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Fibroblast growth factor liposome lyophilized powder for preventing and treating alopecia, and preparation method thereof

A fibroblast and growth factor technology, applied in the field of fibroblast growth factor liposome freeze-dried powder and its preparation, can solve problems such as inability to guarantee stability, and achieves increased hair follicle drug distribution, improved transdermal absorption capacity, The effect of improving stability

Active Publication Date: 2017-11-10
朗肽生物制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no guarantee that the addition of azone will still have good stability to the encapsulated drug

Method used

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  • Fibroblast growth factor liposome lyophilized powder for preventing and treating alopecia, and preparation method thereof
  • Fibroblast growth factor liposome lyophilized powder for preventing and treating alopecia, and preparation method thereof
  • Fibroblast growth factor liposome lyophilized powder for preventing and treating alopecia, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 13

[0047] Example 13: Liposome Characterization

[0048] (1) Particle size measurement: After the finished products prepared in Examples 1 to 12 and the finished products prepared in comparative examples were dispersed in 10mM PBS, respectively, the particle size and particle size were measured by NICOMP 380 particle size analyzer and JEM-2000 transmission electron microscope respectively. Form and particle size results are shown in Table 2 and attached Figure 1a , 1b And attached Figure 2a , 2b shown. Visible, the particle diameter of prepared liposome finished product is between 99.5nm~800nm, wherein the finished product (particle diameter is 95.5nm) prepared by comparative example, the finished product prepared by embodiment 1 has larger particle diameter, and its particle diameter is 99.8nm, transmission electron microscopy showed an obvious gel core structure. The weight ratio of phospholipid to cholesterol has an important influence on maintaining the spherical shape ...

Embodiment 14

[0052] Example 14: Liposome Stability

[0053] The MTT method was used to detect the effect on the proliferation of HaCaT cells before and after being placed at 37°C for 2 weeks, and the percentage of liposome preparation in vitro activity was evaluated. Among them, the HaCaT cell culture medium is prepared by volume ratio of 89% RPMI-1640 medium, 10% FBS and 1% penicillin-streptomycin. 2 cultivated in an incubator. Take HaCaT cells in the logarithmic growth phase, digest with 0.25% trypsin, inoculate in a 96-well plate with about 5000 cells per well, and after incubation for 24 hours, add PBS (control sample), the finished product of Comparative Example, and the finished products of Examples 1 to 12 respectively. and bFGF solution treatment, incubated for 72 hours, the control sample was not treated, the finished products of the comparative examples, the finished products of Examples 1-12 and the bFGF solution were incubated for 72 hours, and 20 μL of 5.0 g L-1 MTT solution ...

Embodiment 15

[0054] Embodiment 15: experiment of promoting hair follicle growth in vitro

[0055] Complete rat hair follicles were selected under a dissecting microscope. The isolated complete hair follicles were cultured in a 35mm small petri dish, and the growth rate of the hair follicles cultured in vitro was divided into 5 groups, with 10 hair follicles in each group, and the experimental group had 6-7ml of conventional culture medium (containing 2ml of glutamine). The finished products of Examples 1-12 were directly dispersed in 5 ml of 10 mM phosphate buffer (pH 7.4) as the test group, and the conventional hair follicle culture solution as the negative control group. After adding 100 μL of test solution, culture in an incubator at 31°C with a certain humidity, observe the growth of hair follicles under an inverted microscope every day, take pictures and measure the growth length. After 8 days of culture, the measured hair follicle length was subtracted from the hair follicle length ...

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Abstract

The present invention discloses fibroblast growth factor liposome lyophilized powder for preventing and treating alopecia, wherein the raw material components comprise fibroblast growth factor, silk fibroin, phospholipid, cholesterol and a transdermal absorption accelerator. According to the present invention, by adding the transdermal absorption accelerator into the liposome lyophilized powder, the transdermal absorption of the fibroblast growth factor is effectively improved so as to increase the drug distribution in the hair follicles, such that the fibroblast growth factor liposome lyophilized powder can be used for preventing and treating alopecia; and the preparation can be directly dispersed by physiological saline and then is sprayed at the scalp, can further be added to the gel matrix to prepare the lipid gel for being coated on the scalp, and has the alopecia prevention and treatment effect.

Description

technical field [0001] The invention relates to the field of fine chemicals, in particular to a fibroblast growth factor liposome freeze-dried powder for preventing and treating hair loss and a preparation method thereof. Background technique [0002] Skin consists of epidermis, dermis, subcutaneous fat, and skin appendages. The skin absorbs external substances mainly through three ways, namely the stratum corneum, the pilosebaceous glands and the orifices of the sweat glands. Under normal circumstances, the skin only absorbs small-molecule lipophilic substances, and the absorption of external macromolecular protein drugs is very limited. At present, it has been found that fibroblast growth factors such as aFGF, bFGF, KGF, FGF-5, FGF-9, etc. can induce the ability to proliferate and regenerate hair follicles, sebaceous glands, and sweat glands, and have the ability to prevent and treat hair loss. However, due to its poor lipophilicity, large molecular weight, and poor skin...

Claims

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Application Information

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IPC IPC(8): A61K9/19A61K9/127A61K38/18A61K47/42A61K47/22A61K47/20A61K47/10A61P17/14
CPCA61K9/0014A61K9/127A61K9/19A61K38/1825A61K47/10A61K47/20A61K47/22A61K47/42
Inventor 徐荷林付廷灵肖健赵应征张元方欧智慧
Owner 朗肽生物制药股份有限公司
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