Cancellous constructs, cartilage particles and combinations of cancellous constructs and cartilage particles

a cancellous and cartilage technology, applied in the field of cancellous constructs, cartilage particles and combinations of cancellous constructs and cartilage particles, can solve the problems of articular cartilage lesions generally not healing, pain or severe restriction of joint movement, etc., and achieve the effect of reducing fibrous tissue formation and improving chondrogenesis

Inactive Publication Date: 2009-12-24
MUSCULOSKELETAL TRANSPLANT FOUND INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0006]The present invention is directed towards a particulate allograft cartilage material, which may optionally be incorporated into an allograft cancellous bone construct, that exhibits improved chondrogenesis and decreased fibrous tissue formation in both in vivo and in vitro environments. The cartilage defect repair material includes lyophilized, freeze-milled allograft cartilage particles having a size within a range of from about 10 microns to about 210 microns.

Problems solved by technology

If the lining becomes worn or damaged resulting in lesions, joint movement may be painful or severely restricted.
Whereas damaged bone typically can regenerate successfully, articular cartilage regeneration is quite limited because of its limited regenerative and reparative abilities.
Articular cartilage lesions generally do not heal, or heal only partially under certain biological conditions, due to the lack of nerves, blood vessels and a lymphatic system.
The limited reparative capabilities of articular cartilage usually results in the generation of repair tissue that lacks the structure and biomechanical properties of normal articular cartilage.
Generally, the healing of the defect results in a fibrocartilaginous repair tissue that lacks the structure and biomedical properties of articular cartilage and degrades over the course of time.
These lesions are difficult to treat because of the distinctive structure and function of articular cartilage.
Osteoarthritis is the leading cause of disability and impairment in middle-aged and older individuals, entailing significant economic, social and psychological costs.

Method used

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  • Cancellous constructs, cartilage particles and combinations of cancellous constructs and cartilage particles
  • Cancellous constructs, cartilage particles and combinations of cancellous constructs and cartilage particles
  • Cancellous constructs, cartilage particles and combinations of cancellous constructs and cartilage particles

Examples

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Effect test

example 1

Measurement of Demineralized Construct Porosity

[0252]The percentage of porosity and average surface pore diameter of a cancellous construct demineralized cap member according to the present invention can be determined utilizing a microscope / infrared camera and associated computer analysis. The microscope / infrared camera was used to produce the images of FIGS. 34A and 34B, which provide a visual assessment of the porosity of the demineralized cap member of the constructs of the present invention. Such images were analyzed using suitable microscopy and image analysis software, for example, Image Pro Plus. The number and diameter of pores and the relative porosity of a demineralized cap member of the construct can be characterized using techniques known to those skilled in the art.

[0253]It is noted that the number and diameter of pores and the relative porosity of the demineralized cap members will vary from one tissue donor to another, and even within the tissue of one tissue donor, b...

example 2

Tissue Extraction and Particularization

[0254]A process of cartilage particle extraction may be applied to any of a number of different soft tissue types (for example, meniscus tissue). In one embodiment, cartilage is recovered from deceased human donors, and the tissue is treated with a soft tissue process.

[0255]Fresh articular cartilage is removed from a donor using a scalpel, taking care to remove the cartilage so that the full thickness of the cartilage is intact (excluding any bone). Removed cartilage is then packaged in double Kapak® bags for storage until ready to conduct chemical cleaning of the allograft tissue. In one example, the cartilage can be stored in the refrigerator for 24-72 hours or in the freezer (e.g., at a temperature of −70° C.) for longer-term storage.

[0256]Chemical cleaning of cartilage tissue is then conducted according to method known by those skilled in the art. Subsequent to chemical cleaning, the cartilage is lyophilized, so as to reduce the water conte...

example 3

Extraction of Proteins from Human Cartilage Using Extraction and Subsequent Dialysis

[0261]In another example, growth factors may be physically and / or chemically isolated from cartilage particles, and dialyzed using a suitable agent. The growth factors are thereby isolated for subsequent analysis and / or quantification. In one embodiment, 0.3 g of cartilage particles were weighed out for each donor. The cartilage particles were transferred to tubes containing 5 ml of extraction solution (4M Guanidine HCl in Tris HCl). The cartilage particles were incubated at 4° C. on an orbital shaker at 60 RPM for 24 hours, followed by dialysis (8k MWCO membrane dialysis tube) in 0.05M Tris HCl or PBS for 15 hrs. at 4° C. The dialysis solution was then replaced and the dialysis continued for another 8 hrs. at 4° C. The post-dialysis extracts were stored at −70° C. until the ELISA was run.

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Abstract

Constructs that are at least partially constructed of allograft cancellous bone are disclosed, along with cartilage particles that may be used with the constructs for repairing articular cartilage defects. A multi-piece construct includes a base member, a cap member and at least one pin that secures the cap member to the base member. The base member may be constructed of mineralized cancellous bone, and is used to replace the subchondral bone removed when a surgeon cuts a bore in the area of an adjacent cartilage defect. The base member includes a blind bore and first and second through-going transverse bores in opposite sides of a wall of the base member. The cap member includes an upper section that has a thickness that is similar to that of a patient's surrounding articular cartilage layer and a stem depending from the upper section that is dimensioned to be received in and by the blind bore of the base member. The stem includes a transverse through-going bore, which may be aligned with the transverse through-going bores of the base member to receive the pin therein when the construct has been assembled. The cap member is at least partially formed of demineralized allograft cancellous bone, into which a mixture containing lyophilized, freeze-milled allograft cartilage particles may be infused for the repair of articular cartilage defects. The cartilage particles have a size within a range of from about 10 microns to about 210 microns.

Description

RELATED APPLICATIONS[0001]This application is a continuation-in-part of (i) U.S. patent application Ser. No. 11 / 657,042 filed Jan. 24, 2007; (ii) U.S. patent application Ser. No. 12 / 043,001 filed Mar. 5, 2008; (iii) U.S. patent application Ser. No. 12 / 328,306 filed Dec. 4, 2008; and (iv) U.S. patent application Ser. No. 12 / 079,629 filed Mar. 26, 2008, which is a divisional of U.S. patent application Ser. No. 10 / 960,960 filed Oct. 12, 2004, now abandoned; and also claims priority under 35 U.S.C. § 119(e) to (v) U.S. Provisional Patent Application Ser. No. 61 / 189,252 filed Aug. 15, 2008, and (i) U.S. Provisional Patent Application Ser. No. 61 / 205,433 filed Jan. 15, 2009. This application is also related to the following commonly owned patent applications: (a) U.S. Provisional Patent Application Ser. No. 60 / 904,809 filed Mar. 6, 2007, and (b) U.S. Provisional Patent Application Ser. No. 60 / 996,800 filed Dec. 5, 2007. All of the foregoing related patent applications are incorporated by ...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61F2/28A61F2/02
CPCA61B17/00491A61F2/28A61L2430/06A61F2/30756A61F2/3094A61F2/3859A61F2/3872A61F2002/2817A61F2002/2839A61F2002/2842A61F2002/30057A61F2002/30059A61F2002/30062A61F2002/30224A61F2002/30225A61F2002/30227A61F2002/30233A61F2002/30235A61F2002/30327A61F2002/30331A61F2002/30354A61F2002/30387A61F2002/30448A61F2002/30459A61F2002/30461A61F2002/30492A61F2002/30604A61F2002/30759A61F2002/30762A61F2002/30764A61F2002/30772A61F2002/30785A61F2002/30789A61F2002/30795A61F2002/3085A61F2002/30932A61F2002/3096A61F2002/4635A61F2002/4646A61F2002/4649A61F2210/0004A61F2220/0025A61F2220/0033A61F2220/005A61F2220/0066A61F2220/0075A61F2230/0069A61F2250/0039A61F2310/00365A61L27/3608A61L27/3612A61L27/3654A61L27/48A61L27/54A61L2300/414A61L2300/43C08L89/00A61F2002/2835A61P19/04
Inventor TRUNCALE, KATHERINE G.SEMLER, ERIC J.GERTZMAN, ARTHUR A.SUNWOO, MOON HAETOMFORD, WILLIAM W.SHIKHANOVICH, ROMANCALLAHAN, ALEX B.YANNARIELLO-BROWN, JUDITH I.JACOBS, MORRIS L.MUNSON, JOHN C.HUANG, YEN-CHEN
Owner MUSCULOSKELETAL TRANSPLANT FOUND INC
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