Cancellous constructs, cartilage particles and combinations of cancellous constructs and cartilage particles

a cancellous and cartilage technology, applied in the direction of prosthesis, drug compositions, peptide/protein ingredients, etc., can solve the problems of articular cartilage lesions generally not healing, pain or severe restriction of joint movement, and limited articular cartilage regeneration, so as to improve chondrogenesis and reduce fibrous tissue formation

Inactive Publication Date: 2011-03-24
MUSCULOSKELETAL TRANSPLANT FOUND INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the lining becomes worn or damaged resulting in lesions, joint movement may be painful or severely restricted.
Whereas damaged bone typically can regenerate successfully, articular cartilage regeneration is quite limited because of its limited regenerative and reparative abilities.
Articular cartilage lesions generally do not heal, or heal only partially under certain biological conditions, due to the lack of nerves, blood vessels and a lymphatic system.
The limited reparative capabilities of articular cartilage usually results in the generation of repair tissue that lacks the structure and biomechanical properties of normal articular cartilage.
Generally, the healing of the defect results in a fibrocartilaginous repair tissue that lacks the structure and biomedical properties of articular cartilage and degrades over the course of time.
These lesions are difficult to treat because of the distinctive structure and function of articular cartilage.
Osteoarthritis is the leading cause of disability and impairment in middle-aged and older individuals, entailing significant economic, social and psychological costs.

Method used

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  • Cancellous constructs, cartilage particles and combinations of cancellous constructs and cartilage particles
  • Cancellous constructs, cartilage particles and combinations of cancellous constructs and cartilage particles
  • Cancellous constructs, cartilage particles and combinations of cancellous constructs and cartilage particles

Examples

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example 1

Measurement of Demineralized Construct Porosity

[0252]The percentage of porosity and average surface pore diameter of a cancellous construct demineralized cap member according to the present invention can be determined utilizing a microscope / infrared camera and associated computer analysis. The microscope / infrared camera was used to produce the images of FIGS. 34A and 34B, which provide a visual assessment of the porosity of the demineralized cap member of the constructs of the present invention. Such images were analyzed using suitable microscopy and image analysis software, for example, Image Pro Plus. The number and diameter of pores and the relative porosity of a demineralized cap member of the construct can be characterized using techniques known to those skilled in the art.

[0253]It is noted that the number and diameter of pores and the relative porosity of the demineralized cap members will vary from one tissue donor to another, and even within the tissue of one tissue donor, b...

example 2

Tissue Extraction and Particularization

[0254]A process of cartilage particle extraction may be applied to any of a number of different soft tissue types (for example, meniscus tissue). Cartilage is recovered from deceased human donors, and the tissue is treated with a soft tissue processing system for bioburden reduction, for example, as disclosed in U.S. Patent Application Publication No. US 2006 / 0275377 A1 of U.S. patent application Ser. No. 11 / 375,026, which is incorporated by reference herein in its entirety.

[0255]Fresh articular cartilage is removed from a donor using a scalpel, taking care to remove the cartilage so that the full thickness of the cartilage is intact (excluding any bone). Removed cartilage is then packaged in double Kapak® bags for storage until ready to conduct chemical cleaning of the allograft tissue, for example, in accordance with U.S. Patent Application Publication No. US 2006 / 0275377 A1. In one example, the cartilage can be stored in the refrigerator for...

example 3

Extraction of Proteins from Human Cartilage Using Extraction and Subsequent Dialysis

[0261]In another example, growth factors may be physically and / or chemically isolated from cartilage particles, and dialyzed using a suitable agent. The growth factors are thereby isolated for subsequent analysis and / or quantification. In one embodiment, 0.3 g of cartilage particles were weighed out for each donor. The cartilage particles were transferred to tubes containing 5 ml of extraction solution (4M Guanidine HCl in Tris HCI). The cartilage particles were incubated at 4° C. on an orbital shaker at 60 RPM for 24 hours, followed by dialysis (8 k MWCO membrane dialysis tube) in 0.05M TrisHCl or PBS for 15 hrs. at 4° C. The dialysis solution was then replaced and the dialysis continued for another 8 hrs. at 4° C. The post-dialysis extracts were stored at −70° C. until the ELISA was run.

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Abstract

Constructs that are at least partially constructed of allograft cancellous bone are disclosed, along with cartilage particles that may be used with the constructs for repairing articular cartilage defects. A multi-piece construct includes a base member, a cap member and at least one pin that secures the cap member to the base member. The base member may be constructed of mineralized cancellous bone, and is used to replace the subchondral bone removed when a surgeon cuts a bore in the area of an adjacent cartilage defect. The base member includes a blind bore and first and second through-going transverse bores in opposite sides of a wall of the base member. The cap member includes an upper section that has a thickness that is similar to that of a patient's surrounding articular cartilage layer and a stem depending from the upper section that is dimensioned to be received in and by the blind bore of the base member. The stem includes a transverse through-going bore, which may be aligned with the transverse through-going bores of the base member to receive the pin therein when the construct has been assembled. The cap member is at least partially formed of demineralized allograft cancellous bone, into which a mixture containing lyophilized, freeze-milled allograft cartilage particles may be infused for the repair of articular cartilage defects. The cartilage particles have a size within a range of from about 10 microns to about 210 microns.

Description

RELATED APPLICATIONS[0001]This application is a divisional application of U.S. patent application Ser. No. 12 / 381,072, filed Mar. 5, 2009, which is a continuation-in-part of (i) U.S. patent application Ser. No. 11 / 657,042, filed Jan. 24, 2007; (ii) U.S. patent application Ser. No. 12 / 043,001, filed Mar. 5, 2008; (iii) U.S. patent application Ser. No. 12 / 328,306, filed Dec. 4, 2008; and (iv) U.S. patent application Ser. No. 12 / 079,629, filed Mar. 26, 2008, which is a divisional of U.S. patent application Ser. No. 10 / 960,960, filed Oct. 12, 2004, now abandoned, and U.S. patent application Ser. No. 12 / 381,072 claims priority under 35 U.S.C. §119(e) to (a) U.S. Provisional Patent Application Ser. No. 61 / 189,252, filed Aug. 15, 2008; and (b) U.S. Provisional Patent Application Ser. No. 61 / 205,433, filed Jan. 15, 2009; and is related to (c) U.S. Provisional Patent Application Ser. No. 60 / 904,809, filed Mar. 6, 2007; and (d) U.S. Provisional Patent Application Ser. No. 60 / 996,800, filed De...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K35/32A61K9/00A61K38/20A61P19/04
CPCA61B17/00491A61F2/28A61L2430/06A61F2/30756A61F2/3094A61F2/3859A61F2/3872A61F2002/2817A61F2002/2839A61F2002/2842A61F2002/30057A61F2002/30059A61F2002/30062A61F2002/30224A61F2002/30225A61F2002/30227A61F2002/30233A61F2002/30235A61F2002/30327A61F2002/30331A61F2002/30354A61F2002/30387A61F2002/30448A61F2002/30459A61F2002/30461A61F2002/30492A61F2002/30604A61F2002/30759A61F2002/30762A61F2002/30764A61F2002/30772A61F2002/30785A61F2002/30789A61F2002/30795A61F2002/3085A61F2002/30932A61F2002/3096A61F2002/4635A61F2002/4646A61F2002/4649A61F2210/0004A61F2220/0025A61F2220/0033A61F2220/005A61F2220/0066A61F2220/0075A61F2230/0069A61F2250/0039A61F2310/00365A61L27/3608A61L27/3612A61L27/3654A61L27/48A61L27/54A61L2300/414A61L2300/43C08L89/00A61F2002/2835A61P19/04
Inventor TRUNCALE, KATHERINE G.SUNWOO, MOON HAEGERTZMAN, ARTHUR A.TOMFORD, WILLIAM W.
Owner MUSCULOSKELETAL TRANSPLANT FOUND INC
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