Cartilage-bone integrated porous bionic scaffold and preparation method thereof

Abstract

The invention relates to a cartilage-bone integrated porous bionic scaffold and a preparation method thereof. The preparation method comprises the following steps: preparing cartilage acellular matrixpowder, and preparing bone tissue demineralized bone matrix powder; preparing gelatin or a collagen solution; preparing gelatin or collagen suspension containing a cartilage acellular matrix, and preparing gelatin or collagen suspension containing a demineralized bone matrix; preparing gelatin or collagen suspension containing the cartilage acellular matrix and the demineralized bone matrix; constructing a cartilage-bone integrated three-dimensional digital model through computer aided design, and forming a cartilage-bone integrated kernel grid framework through three-dimensional printing; fixing the cartilage-bone integrated grid framework in a cylindrical mould, pouring the gelatin or collagen suspension containing the demineralized bone matrix, preparing a bone phase scaffold, pouringthe gelatin or collagen suspension containing the cartilage acellular matrix and the demineralized bone matrix, preparing a junctional phase scaffold, pouring the gelatin or collagen suspension containing the cartilage acellular matrix again, preparing a cartilage phase scaffold, and preparing into the cartilage-bone integrated three-dimensional frozen scaffold.

Description

technical field [0001] The invention relates to biomedical tissue engineering, in particular to a cartilage-bone integrated porous bionic scaffold with a three-dimensional structure and a preparation method thereof. Background technique [0002] Osteochondral composite injuries in joints caused by various reasons such as trauma, disease, and degeneration are common orthopedic clinical diseases, and are increasing year by year in my country. The repair of osteochondral composite defects has a major clinical need. At present, it is difficult to treat this kind of compound injury of articular cartilage combined with subchondral bone, and the effect is not satisfactory. It is urgent to study ideal permanent repair and reconstruction measures. At present, the commonly used clinical treatment methods include autologous osteochondral transplantation, allogeneic osteochondral transplantation and artificial joint replacement. The sources of autologous osteochondral transplantation a...

AI Technical Summary

Problems solved by technology

Among them, the advantages of natural materials are good biocompatibility, easy degradation, easy absorption of degradation products and less toxic side effects, and the disadvantage is that there are differences in structure and performance.

There are many kinds of artificially synthesized scaffold materials, which have attracted widespread attention due to their advantages such as good physical and mechanical properties, controllable degradation, and unlimited sources. However, polymer biomaterials have certain immunogenicity and are prone to inflammation and rejection. Poor water, poor biocompatibility; degraded metabolites are corrosive, etc.

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