Composite support for tissue engineering auricle shape and preparation method thereof

Abstract

The present invention relates to a tissue-engineered auricle-shaped composite scaffold and a preparation method thereof. The method comprises: making cartilage decellularized matrix powder from cartilage tissue, preparing gelatin or collagen suspension containing decellularized cartilage matrix, and performing CT, MRI or Laser scanning and computer-aided design construct a three-dimensional digital model of the human ear shape; 3D printing of PCL substrates prepares a three-dimensional human ear shape auricle grid frame, and prepares a solid positive mold and a solid negative mold corresponding to the shape of the auricle; the three-dimensional human ear Morphology The auricle grid frame is fixed in the middle of the solid male mold and the solid female mold, and the prepared cartilage decellularized matrix gelatin or collagen suspension is perfused, frozen after perfusion, and the solid male mold and the solid female mold are peeled off after freezing , to obtain a three-dimensional frozen scaffold. After the three-dimensional frozen scaffold is freeze-dried, it is immersed in a chemical cross-linking agent for cross-linking. After cross-linking, deionized water is repeatedly washed and soaked to remove the residual chemical cross-linking agent. Auricle shape support.

Description

technical field [0001] The invention relates to biomedical tissue engineering, in particular to a tissue engineering auricle shape support with a three-dimensional structure and a preparation method thereof. Background technique [0002] Auricle defects or loss due to various reasons such as congenital microtia, trauma and tumors are common clinically. The auricle is one of the main features of the human face, and its defect or loss will seriously affect the patient's aesthetics and mental health. The auricle of the human body is mainly composed of skin and cartilage. Since the skin tissue is easily obtained by local skin expansion and / or skin grafting, the key difficulty in ear reconstruction for auricle defects lies in how to obtain an ear with good shape and function. Rebuild the bracket. The current clinical ear reconstruction methods are mainly based on autologous costal cartilage carving or high-density polyethylene (HDPE) artificial ear brackets. The disadvantages a...

AI Technical Summary

Problems solved by technology

At present, polyglycolic acid / polylactic acid (polyglycolic acid / polylactic acid, PGA / PLA) scaffolds combined with large animal chondrocytes have been used to successfully construct cartilage with accurate human ear shape in vitro, but long-term observation in vivo has found that due to This scaffold is a polymer synthetic material, and the small amount of undegraded scaffold remaining in the cartilage can easily cause aseptic inflammatory reactions, resulting in unstable cartilage regeneration in vivo

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