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Cartilage-bone integrated porous bionic scaffold and preparation method thereof

ActiveCN110180025ASolve the problem of not being able to accurately control the shape of 3D printingAdd control functionTissue regenerationProsthesisComputer Aided DesignAcellular matrix
The invention relates to a cartilage-bone integrated porous bionic scaffold and a preparation method thereof. The preparation method comprises the following steps: preparing cartilage acellular matrixpowder, and preparing bone tissue demineralized bone matrix powder; preparing gelatin or a collagen solution; preparing gelatin or collagen suspension containing a cartilage acellular matrix, and preparing gelatin or collagen suspension containing a demineralized bone matrix; preparing gelatin or collagen suspension containing the cartilage acellular matrix and the demineralized bone matrix; constructing a cartilage-bone integrated three-dimensional digital model through computer aided design, and forming a cartilage-bone integrated kernel grid framework through three-dimensional printing; fixing the cartilage-bone integrated grid framework in a cylindrical mould, pouring the gelatin or collagen suspension containing the demineralized bone matrix, preparing a bone phase scaffold, pouringthe gelatin or collagen suspension containing the cartilage acellular matrix and the demineralized bone matrix, preparing a junctional phase scaffold, pouring the gelatin or collagen suspension containing the cartilage acellular matrix again, preparing a cartilage phase scaffold, and preparing into the cartilage-bone integrated three-dimensional frozen scaffold.
Owner:SHANGHAI TISSUE ENG LIFE SCI
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