Cartilage-bone integrated porous bionic scaffold and preparation method thereof

Abstract

The invention relates to a cartilage-bone integrated porous bionic scaffold and a preparation method thereof. The preparation method comprises: preparing cartilage decellularized matrix powder, preparing bone tissue decalcified bone matrix powder; preparing gelatin or collagen solution; preparing cartilage-containing decellularized matrix Prepare gelatin or collagen suspension containing decalcified bone matrix; prepare gelatin or collagen suspension containing cartilage decellularized matrix and decalcified bone matrix; construct cartilage-bone by computer-aided design An integrated three-dimensional digital model, which is 3D printed into a cartilage-bone integrated core grid frame; the cartilage-bone integrated grid frame is fixed in a cylindrical mold and perfused with gelatin or collagen suspension containing decalcified bone matrix , prepare the bone phase scaffold, perfuse the gelatin or collagen suspension containing cartilage decellularized matrix and decalcified bone matrix, prepare the junctional phase scaffold, and then perfuse the gelatin or collagen suspension containing cartilage decellularized matrix, prepare the cartilage phase scaffold, make Chondrogenic-osseointegrated three-dimensional cryo-stent.

Description

technical field [0001] The invention relates to biomedical tissue engineering, in particular to a cartilage-bone integrated porous bionic scaffold with a three-dimensional structure and a preparation method thereof. Background technique [0002] Osteochondral composite injuries in joints caused by various reasons such as trauma, disease, and degeneration are common orthopedic clinical diseases, and are increasing year by year in my country. The repair of osteochondral composite defects has a major clinical demand. At present, it is difficult to treat this kind of compound injury of articular cartilage combined with subchondral bone, and the effect is not satisfactory. It is urgent to study ideal permanent repair and reconstruction measures. At present, the commonly used clinical treatment methods include autologous osteochondral transplantation, allogeneic osteochondral transplantation and artificial joint replacement. The sources of autologous osteochondral transplantation...

AI Technical Summary

Problems solved by technology

Among them, the advantages of natural materials are good biocompatibility, easy degradation, easy absorption of degradation products and less toxic side effects, and the disadvantage is that there are differences in structure and performance.

There are many kinds of artificially synthesized scaffold materials, which have attracted widespread attention due to their advantages such as good physical and mechanical properties, controllable degradation, and unlimited sources. However, polymer biomaterials have certain immunogenicity and are prone to inflammation and rejection. Poor water, poor biocompatibility; degraded metabolites are corrosive, etc.

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