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Gel-poly(lactide-co-glycolide) (PLGA) two-phase gradient transition cartilage-bone repair material and preparation thereof

A gradient transition and bone repair technology, applied in bone implants, medical science, prostheses, etc., can solve the problems that the multiphase gradient scaffold cannot realize the continuous gradient of the components in the transition zone, and is easy to fall off and separate, so that it is not easy to separate and fall off , Simple preparation process, good biocompatibility effect

Inactive Publication Date: 2014-03-26
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Most of the existing preparations of multiphase gradient scaffolds for cartilage-bone repair cannot realize the continuous gradient of the components in the transition zone, and the layers are easy to fall off and separate. The preparation method of the rubber phase partially permeating into the PLGA porous scaffold to make the two phases tightly combined has not been reported in the literature

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1. Weigh 0.11g of gelatin microspheres with a diameter of 350-450μm and place them in a columnar template with a diameter of 6mm, then press them into a glass mold with a tapered tip (5mm in diameter, 2mm in height), and drop 100μL A solution with a ratio of ethanol to water of 8 / 2 was cross-linked at 37°C for 15 minutes to obtain a gelatin skeleton.

[0017] 2. Immerse the gelatin skeleton in 7% poly(lactide- co -glycolide) (PLGA) in dichloromethane solution, soak for 12 hours, and remove the gas in the pores in a vacuum oven to ensure that the solution enters completely. Take out the stent, blot the surface solution dry, separate the phases in the refrigerator (-20°C) for 12 hours, and replace with ethanol for 12 hours. Finally, the gelatin skeleton was washed away in water at 37° C. to obtain a poly(lactide-lactide- co - glycolide) (PLGA) porous scaffold. The scaffolds were dried in a vacuum oven and stored at -20 °C.

[0018] 3. Weigh 8.9mg of carboxymethyl chit...

Embodiment 2

[0021] 1. Weigh 0.15g of gelatin microspheres with a diameter of 350~450μm and place them in a columnar template with a diameter of 6mm, then press them into a glass mold with a tapered tip (5mm in diameter, 3mm in height), and drop 100μL A solution with a ratio of ethanol to water of 8 / 2 was cross-linked at 37°C for 30 minutes to obtain a gelatin skeleton.

[0022]2. Immerse the gelatin skeleton in 6% poly(lactide- co -glycolide) (PLGA) in dichloromethane solution, soak for 12 hours, and remove the gas in the pores in a vacuum oven to ensure that the solution enters completely. Take out the stent, blot the surface solution dry, separate the phases in the refrigerator (-20°C) for 12 hours, and replace with ethanol for 12 hours. Finally, the gelatin skeleton was washed away in water at 37° C. to obtain a poly(lactide- co - glycolide) (PLGA) porous scaffold. The scaffolds were dried in a vacuum oven and stored at -20°C.

[0023] 3. Weigh 5.6 mg of carboxymethyl chitosan modi...

Embodiment 3

[0026] 1. Weigh 0.11g of gelatin microspheres with a diameter of 350-450μm and place them in a columnar template with a diameter of 6mm, then press them into a glass mold with a tapered tip (5mm in diameter, 2mm in height), and drop 100μL A solution with a ratio of ethanol to water of 8 / 2 was cross-linked at 37°C for 15 minutes to obtain a gelatin skeleton.

[0027] 2. Immerse the gelatin skeleton in 9% poly(lactide- co -glycolide) (PLGA) in dichloromethane solution, soak for 12 hours, and remove the gas in the pores in a vacuum oven to ensure that the solution enters completely. Take out the stent, blot the surface solution dry, separate the phases in the refrigerator (-20°C) for 12 hours, and replace with ethanol for 12 hours. Finally, the gelatin skeleton was washed away in water at 37° C. to obtain a poly(lactide-lactide- co - glycolide) (PLGA) porous scaffold. The scaffolds were dried in a vacuum oven and stored at -20°C.

[0028] 3. Weigh 12 mg of carboxymethyl chito...

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Abstract

The invention discloses a gel-poly(lactide-co-glycolide) (PLGA) two-phase gradient transition cartilage-bone repair material and preparation thereof. The material is cylindrical and consists of an upper material and a lower material, wherein the upper material is glycidyl methacrylate-modified carboxymethyl chitosan and gelatin; the lower material is porous PLGA. The preparation process comprises the following steps: preparing a gelatin microsphere serving as a pore-forming agent into a PLGA porous scaffold of which one end face is a conical concave surface; preparing a gel solution by using the modified chitosan and modified gelatin; dripping the gel solution on the PLGA porous scaffold of the conical concave surface, inverting, performing ultraviolet light cross-linking to obtain the gel-PLGA two-phase gradient transition cartilage-bone repair material. The material has high biocompatibility and biodegradability, is in a two-phase gradient transition structure, is tightly combined without drop and is simple in preparation process.

Description

technical field [0001] The present invention relates to a gel-poly(lactide- co The invention discloses a cartilage-bone repairing scaffold material with two-phase gradient transition of -glycolide) (PLGA) and a preparation method thereof, belonging to the field of cartilage-bone interface tissue repairing biomaterials. Background technique [0002] Bone interface tissues such as cartilage-bone, tendon-bone, and ligament-bone are heterogeneous in anatomical structure and tissue composition, but have the characteristics of continuous gradient changes in structure and composition. Therefore, in order to achieve a good repair of interface tissue, an ideal design is required. A functionalized gradient scaffold (Leong K F, Chua C K, Sudarmadji N, Yeong W Y, Journal of the Mechanical Behavior of Biomedical Materials, 2008, 1(2): 140-152). [0003] For the cartilage-bone interface, there are significant differences in composition, structure, and mechanical properties. At present, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/26A61L27/24A61L27/20A61L27/18A61L27/50A61F2/28
Inventor 袁晓燕韩凤选赵蕴慧杨晓玲
Owner TIANJIN UNIV
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