Cartilage-bone-bone marrow composite tissue structure and method based on living cell 3D printing

Abstract

The invention discloses a cartilage-bone-bone marrow composite tissue structure and method based on 3D printing of living cells. The biological information of bone tissue is collected, input into a computer for bionic modeling, and the preparation is suitable for cartilage, osteogenesis, mesenchymal stem, and blood vessels. Bio-ink for cells such as endothelium; prepare hard materials for printing dense bone areas; prepare various casting agents; inoculate living cells in corresponding bio-inks at a certain density; load various types of printing inks separately 3D printing is carried out after setting the printing parameters in the printer silo, and the printed bone tissue is cultured in a tissue engineering bionic incubator. After a certain period of time, the cast is removed at different times by temperature control, enzyme control or light control. The artificial blood vessels are grafted outside the cartilage-bone-bone marrow composite tissue, and cultured in a tissue engineering bionic incubator with continuous cycle perfusion to realize the pre-maturation of the printed tissue. It realizes the long-term survival and biological function of 3D printed bone tissue.

Description

technical field [0001] The invention belongs to biomedical engineering, in particular to a cartilage-bone-bone marrow composite tissue structure and method based on living cell 3D printing. Background technique [0002] Due to the high incidence of bone defects caused by war trauma and disease, a large number of bone graft prostheses are required clinically, and bone tissue transplantation ranks second among all tissue and organ transplants. At present, bone graft prostheses are mostly composed of metals and high molecular polymers, which only mimic the shape and appearance of defective bones, but have no biological activity, which often leads to serious defects in physiological functions after transplantation. In recent years, the rapid development of 3D printing technology provides the possibility to solve the above problems. Bio-3D printing is a promising method for constructing engineered human tissue, which can theoretically achieve a high degree of biomimetic tissue s...

AI Technical Summary

Problems solved by technology

[0005] Another object of the present invention is to address the deficiencies in the prior art, to provide a combination of hard and soft natural polymer materials, and to use the differences in physical and chemical properties, curing mechanisms and time of different materials to establish multi-material composite molding. The multi-layer printing process solves the problem of mutual interference of materials in the complex structure forming process, and combines high bionic structure modeling and multi-degree-of-freedom robotic arm path planning to realize the 3D printing bionic modeling of cartilage-bone-bone marrow complex structures

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