35results about How to "Adequate perfusion" patented technology

The invention relates to a dry type making method for animal specimen that includes six steps: collecting material, reshaping antisepsis fix and tube cavity cast, alcohol dewatering fix, glycerol process, turpentine impregnation and air drying, material repairing tinction preservation or seal up for keeping. Thereinto, in step 1, the animal material is bleed and killed via health quarantine, in step 2, potting it with the formalin with colorless glycerin and phenol or alcohol solution, in step 3, controlling the concentration of every step and fixed time, in step 4, marinating it with glycerol moisture absorption soften liquid, and transfer it from low concentration to high concentration of glycerin, and the time is prolonged gradually, in step 5, treating it with turpentine continually, in step 6, printing it. The invention improves the present technique, the quality of specimen is improved greatly, and that can keep the original state of material, the color and the luster. The dried specimen is sanitation without peculiar smell, and good at handle and quality.

An intravascular device and method of constructing an intravascular device. The device has a proximal portion which is stiffer than a distal portion. The device of the present invention may also be advanced through small vessels without the aid of a guidewire although a guidewire may be used when necessary. The device may be manufactured in a number of different ways and a preferred method is to use an expanded PTFE liner at the distal portion and an etched PTFE liner along the proximal portion. The device also has a number of different jacket sections, preferably at least four, with increasing durometer towards the proximal end and a braided section with varying pic along the length.

The invention relates to the technical field of lithium ion batteries, in particular to a method for measuring electrolyte distribution in a lithium ion battery core. The method specifically comprises the following steps of injecting electrolyte containing a tracer element into a to-be-measured lithium ion battery, measuring the content of the tracer element in a to-be-measured part of the lithium ion battery core by utilization of an ion tracing method, calculating actual electrolyte distribution quantity of each region of the to-be-measured part, and obtaining an electrolyte distribution difference value by means of calculating a difference between the actual electrolyte distribution quantity and theoretical electrolyte absorption quantity. The method disclosed by the invention has the advantages that the effects of simplicity and easiness in implementation are achieved; the distribution quantity condition of the electrolyte on a battery anode film sheet, a battery cathode film sheet and an electronic insulating film can be respectively tracked and characterized, so that not only can the injecting quantity of the electrolyte of the battery with different sizes or different materials be predicted, and the condition that sufficient electrolyte is injected into the battery can be guaranteed, but also the distribution tracking on the absorption of the electrolyte of all parts of the battery anode sheet and the battery anode sheet can be performed.

The invention discloses a cartilage-bone-marrow composite tissue structure based on 3D living cell printing and a method of the cartilage-bone-marrow composite tissue structure. Biological informationof bone tissue is collected and input into a computer for biomimetic modeling, and bio-ink suitable for cells such as cartilage, osteogenesis, mesenchymal stem, vascular endothelium, etc., is prepared; hard materials for printing dense bone area are prepared; various casting agent is prepared; living cells are inoculated into corresponding bio-ink at a certain density; and various types of printing ink are loaded into a printer bin correspondingly, 3D printing is carried out after setting printing parameters, and printed bone tissue is cultured in a tissue engineering bionic incubator. Aftera certain time, the method of temperature control or enzyme control or light control is used to remove casting agent materials at different times, artificial blood vessels are grafted on the outside of the cartilage-bone-marrow composite tissue, and continuous circulation perfusion cultivation is carried out in the tissue engineering biomimetic incubator, so as to achieve the pre-maturation of printed tissue, and the long-term survival and biological function of 3D printing bone tissue can be realized.

The invention provides high-temperature RTM (resin transfer molding) integrated molding equipment. The equipment comprises a resin tank, a high-temperature resin distributing pipeline and a curing tank, wherein the resin tank is arranged at the top of the curing tank in an integrated manner, the high-temperature resin distributing pipeline is arranged at the top part in the curing tank to meet the requirements of heat preservation property of the high-temperature resin distributing pipeline and the flowing property of the rein injection runners, the top part of the curing tank is provided with an opening at a glue outlet of the resin tank, three or more product molds can be placed in the curing tank, a mold vacuum pipeline is arranged outside the curing tank, the glue outlet of the resin tank is connected with the high-temperature resin distributing pipeline by a discharging valve, the high-temperature resin distributing pipeline is provided with three or more outlets, each outlet is connected with one mold through a resin distributing valve and a resin flowing pipeline, and each mold is connected with the mold vacuum pipeline by a liquid storage tank. According to the invention, high-temperature resin can be ensured to be fully poured and fed, the molding operation is convenient and the production efficiency is improved.

The invention discloses a combined cardiac arrest preserving fluid, comprising St. Thomas fluid and hemoglobin-based oxygen carriers. The fluid can be prepared into a commercial cardiac arrest preserving fluid finished product for sale, or the St. Thomas fluid and the hemoglobin-based oxygen carriers are respectively packaged independently for preparation before use. The cardiac arrest preserving fluid has the advantages of safety and efficiency, simple operation and convenient use, and is mainly used for cardiac arrest and protection in extracorporeal circulation operation.

The invention provides a liver oxygen-carrying mechanical perfusion device which comprises a temperature control container as well as a first centrifugal pump, an oxygenator, a filtration and bubble discharge device, a flow controller, a first pressure sensor and a portal vein perfusion tube that are sequentially connected with one another by a pipeline to form a first perfusion circulation system, wherein the portal vein perfusion tube is connected with a portal vein of a liver. The liver oxygen-carrying mechanical perfusion device also comprises a suprahepatic vena cava tube, a second pressure sensor, an elastic water container and a second centrifugal pump that are sequentially connected with one another by a pipeline to form a second perfusion circulation system, wherein the suprahepatic vena cava tube is connected with a suprahepatic vena cava of the liver. The elastic water container and the second centrifugal pump are additionally arranged at a liver outflow tract to simulate physiological conditions; with the fluctuation of the pressure of the suprahepatic vena cava in a cardiac impulse process, the resistance of the liver outflow tract is regulated, so that the liver perfusion is more sufficient. According to the device, a marginal donor liver can be repaired, so that the liver confirms to the transplantation requirements, therefore, the liver donation source is greatly expanded.

The invention discloses a primary rat or mouse gastric mucosal epithelial cell isolation and culture method. A perfusion method is innovatively introduced into the primary rat or mouse gastric mucosal epithelial cell isolation and culture method, and sufficient perfusion without dead corners is achieved. Prepared compound enzyme digestive juice avoids the problems of low cell viability and poor yield and is capable of digesting more evenly and thoroughly as compared with preheated digestion of traditional single collagenase. Within 12 hours of cell inoculation adherence, a cell culture medium with a pH value of 6-6.5 is adopted, cell adherence rate can be increased greatly and adherence effects are better. The primary rat or mouse gastric mucosal epithelial cell isolation and culture method has the advantages of large total cell number, high isolation degree, high living rate and low contamination probability by bacteria, fungi and other cells as well as capability of achieving subculture so as to provide a favorable cell culture scheme for related fundamental researches.

The invention provide a three-branch single-cylinder hydraulic bidirectional expanding-extrusion device, which comprises upper expanding-extrusion arms, lower broken-end expanding-extrusion arms, support arms, a piston rod, a fixed disk, a moving disk and a cylinder block. The three-branch single-cylinder hydraulic bidirectional expanding-extrusion device is characterized in that in rotation of the three-branch single-cylinder hydraulic bidirectional expanding-extrusion device, a soil layer is cut by cutting edges of the upper expanding-extrusion arms to form a cavity, and the lower wall of a bearing disk cavity is rolled by the lower broken-end expanding-extrusion arms to compact the soil layer; when the cavity is formed, the three-branch single-cylinder hydraulic bidirectional expanding-extrusion device rotates reversely; and the upper wall of the bearing disk cavity is squeezed by arc slants of the upper expanding-extrusion arms to compact the soil layer, and the lower broken-end expanding-extrusion arms scrape fallen residue and rotate reversely to roll the lower wall of the bearing disk cavity for the second time so as to compact the soil layer. Compared with the prior art, the three-branch single-cylinder hydraulic bidirectional expanding-extrusion device has the evident advantages that expanding-extrusion is separately performed, quick, laborsaving and reliably effective, the shape of the formed cavity more meets the load bearing requirement, and the like, and accordingly is well worthy of popularization and application.

The invention discloses slurry with time-variable viscosity and a preparation method thereof and belongs to the technical field of grouting. The slurry with time-variable viscosity comprises 10-30 parts of cement, 0.02-0.1 part of a cellulose solvent, 0.5-2 parts of a synthetic calcareous and siliceous early strength agent, 0.05-0.1 part of an amide stability regulator and 5-10 parts of water. The preparation method comprises the following steps: adding water into the synthetic calcareous and siliceous early strength agent and the amide stability regulator for dissolution; then adding the cellulose solvent; finally, respectively adding the dissolved synthetic calcareous and siliceous early strength agent and amide stability regulator to prepare the slurry with time-variable viscosity. When being used for reinforcement of strong-unloading deep tension fracture rock mass and cataclastic rock mass, the slurry with time-variable viscosity has the characteristics that the early strength is quickly increased, and the late strength and the groutability are high.

The invention relates to an oxygen carrying coronary contrast medium, which comprises a contrast medium and a hemoglobin oxygen carrying carrier. According to the condition whether the contrast medium is mixed with the hemoglobin oxygen carrying carrier or not before using, the oxygen carrying coronary contrast medium has the following two types of: 1, storing the hemoglobin oxygen carrying carrier and the contrast medium respectively, wherein the hemoglobin oxygen carrying carrier is contained in dilution solution which is diluted by a diluent until the hemoglobin concentration is between 0.1 and 5g/dL, the pH value of the dilution solution is between 7.0 and 7.4; and making the hemoglobin oxygen carrying carrier contained in the dilution solution in an oxygen saturation state by oxygenating before using; and 2, forming mixed liquor by using the hemoglobin oxygen carrying carrier and the contrast medium, wherein the proportion of the contrast medium to the hemoglobin oxygen carrying carrier is limited by the hemoglobin concentration which is between 0.1 and 5 g/dL in the mixed liquor, and the pH value of the mixed liquor is between 7.0 and 7.4; and making the hemoglobin oxygen carrying carrier contained in the mixed liquor in the oxygen saturation state by oxygenating before using.

The invention provides a rat hepatic stellate cell separation method, which comprises the following steps of (a) injecting 10-percent chloral hydrate into the abdominal cavity for anaesthetizing a rat, and laparotomizing the abdominal cavity of the rat so as to expose the liver and the portal vein; (b) performing heparin intravenous injection, and preheating perfusion liquid for liver perfusion; (d) taking the liver subjected to sufficient perfusion, cutting the liver into pieces, and performing oscillation digestion in preheated digestion liquid; (e) filtering digested liver tissues through a multilayer sieve screen; performing low-speed centrifugation; sucking supernatant; performing centrifugation again to obtain cell precipitates; and performing once cleaning by cleaning liquid; (f) resuspending the obtained cell precipitates, and performing Nycoden gradient centrifugal separation; (g) cleaning the cells for many times, resuspending the cell precipitates by a complete culture medium, and performing inoculated culture; and (h) performing cell immunofluorescent identification on rat hepatic stellate cells. The rat hepatic stellate cell separation method provided by the invention can be used for obtaining rat hepatic stellate cells which have high yield, high purity, high activity and can be activated.

The invention discloses a semiconductor film with gradually increased hole diameters of different layers and a preparation method thereof, belonging to the technical field of dye-sensitized solar cells. The semiconductor film is composed of three titanium dioxide particle layers, and the hole diameters of the three layers are gradually increased. The semiconductor film is prepared through the following steps of: preparing styrene microspheres; preparing three kinds of slurry applied to different layers by using titanium dioxide, terpineol, ethyl cellulose, ethanol and polystyrene microspheres; taking fluorine-doped tin oxide conductive glass as a substrate; carrying out coating through silk screen printing; and finally calcining so as to obtain a semiconductor film with gradually increased hole diameters of different layers. The semiconductor film and preparation method thereof disclosed by the invention have the advantages that a structure with gradually increased hole diameters of different layers facilitates the penetration of electrolyte and dye liquid; and the semiconductor film is good in light scattering effect, high in photoelectric conversion rate, the production operation process of the production operation process is simple and the semiconductor film is easy to be in industrial production.

The invention provides an isolation and culture method for primary rat aortic smooth muscle cells. With the isolation and culture method provided by the invention, the acquired cells are great in gross quantity and high in both isolation degree and motility rate; the probability of contamination by bacteria, fungi and other cells is low; and the method can realize subculturing, so a good cell culture foundation is laid for related research on blood vessel diseases.

The invention provides a novel CA (cement asphalt) mortar grouting bag which is a rectangular bag made of polypropylene non-woven fabrics. The novel CA mortar grouting bag comprises material inlets, elastic elements, sealing parts, bend grouting pipes and retaining plates. The material inlets are arranged on opposite side surfaces of the grouting bag. One ends of the sealing parts are fixed at the positions where the material inlets are located, and the other ends of the sealing parts are connected with the bend grouting pipes. The plurality of elastic parts are evenly distributed in the grouting bag, two ends of the elastic parts are fixed on the upper surface and the lower surface of the grouting bag respectively, and the natural lengths of the elastic parts are smaller than the distance between a track plate and a concrete base. The retaining plates are fixed at the vertex angle positions of all the side surfaces of the grouting bag. The novel CA mortar grouting bag has the advantages of being simple in structure and low in cost, being capable of ensuring sufficient grouting of CA mortar, closely clinging to the track plate and the concrete base, and improving the grouting effect and the construction quality.

The invention belongs to the technical field of frozen drinks, and particularly relates to a transverse multicolor frozen drink and a preparation method thereof. The preparation method comprises the following steps that S1, a first color layer is prepared through a shell suction process, wherein the shell suction thickness of the first color layer accounts for 1/3 +/-1/30 of the thickness of a longitudinal section; ad S2, other color layers are prepared by adopting a pouring process. According to the transverse multicolor frozen drink prepared by the method, part of a first color layer material and part of a second color layer material are arranged in a crossed manner, so that the problem that the first color layer material is easy to fall off is effectively avoided, and the groove defect does not exist between the first color layer material and the second color layer material. If the shell suction thickness of the first color layer of the frozen drink is small, the taste of the first color layer of the frozen drink is mixed with the taste of the second color layer of the frozen drink, and the characteristic taste is weakened; and if the first color layer of the frozen drink is large in shell suction thickness, the first color layer of the frozen drink is easy to break.