Cartilage-bone-marrow composite tissue structure based on 3D living cell printing and method of cartilage-bone-marrow composite tissue structure

Abstract

The invention discloses a cartilage-bone-marrow composite tissue structure based on 3D living cell printing and a method of the cartilage-bone-marrow composite tissue structure. Biological informationof bone tissue is collected and input into a computer for biomimetic modeling, and bio-ink suitable for cells such as cartilage, osteogenesis, mesenchymal stem, vascular endothelium, etc., is prepared; hard materials for printing dense bone area are prepared; various casting agent is prepared; living cells are inoculated into corresponding bio-ink at a certain density; and various types of printing ink are loaded into a printer bin correspondingly, 3D printing is carried out after setting printing parameters, and printed bone tissue is cultured in a tissue engineering bionic incubator. Aftera certain time, the method of temperature control or enzyme control or light control is used to remove casting agent materials at different times, artificial blood vessels are grafted on the outside of the cartilage-bone-marrow composite tissue, and continuous circulation perfusion cultivation is carried out in the tissue engineering biomimetic incubator, so as to achieve the pre-maturation of printed tissue, and the long-term survival and biological function of 3D printing bone tissue can be realized.

Description

technical field [0001] The invention belongs to biomedical engineering, in particular to a cartilage-bone-bone marrow composite tissue structure and method based on living cell 3D printing. Background technique [0002] Due to the high incidence of bone defects caused by war trauma and disease, a large number of bone graft prostheses are required clinically, and bone tissue transplantation ranks second among all tissue and organ transplants. At present, bone graft prostheses are mostly composed of metals and high molecular polymers, which only mimic the shape and appearance of defective bones, but have no biological activity, which often leads to serious defects in physiological functions after transplantation. In recent years, the rapid development of 3D printing technology provides the possibility to solve the above problems. Bio-3D printing is a promising method for constructing engineered human tissue, which can theoretically achieve a high degree of biomimetic tissue s...

AI Technical Summary

Problems solved by technology

[0005] Another object of the present invention is to address the deficiencies in the prior art, to provide a combination of hard and soft natural polymer materials, and to use the differences in physical and chemical properties, curing mechanisms and time of different materials to establish multi-material composite molding. The multi-layer printing process solves the problem of mutual interference of materials in the complex structure forming process, and combines high bionic structure modeling and multi-degree-of-freedom robotic arm path planning to realize the 3D printing bionic modeling of cartilage-bone-bone marrow complex structures

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