Primary rat or mouse gastric mucosal epithelial cell isolation and culture method

An epithelial cell, separation and culture technology, applied in the field of cell culture, can solve the problems of large tissue cell damage, reduced cell yield and activity, and continuous in-depth research limitations, to improve separation, prevent blood clot adhesion, inhibit Effects of bacterial and fungal contamination
CN105907700AInactive Publication Date: 2016-08-31็Ž‹ๆ™“ๅ†ฐ
2 Cites 7 Cited by

Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Applications(China)
Current Assignee / Owner
็Ž‹ๆ™“ๅ†ฐ
Publication Date
2016-08-31
Estimated Expiration
Not applicable ยท inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
Patent Text Reader

Abstract

The invention discloses a primary rat or mouse gastric mucosal epithelial cell isolation and culture method. A perfusion method is innovatively introduced into the primary rat or mouse gastric mucosal epithelial cell isolation and culture method, and sufficient perfusion without dead corners is achieved. Prepared compound enzyme digestive juice avoids the problems of low cell viability and poor yield and is capable of digesting more evenly and thoroughly as compared with preheated digestion of traditional single collagenase. Within 12 hours of cell inoculation adherence, a cell culture medium with a pH value of 6-6.5 is adopted, cell adherence rate can be increased greatly and adherence effects are better. The primary rat or mouse gastric mucosal epithelial cell isolation and culture method has the advantages of large total cell number, high isolation degree, high living rate and low contamination probability by bacteria, fungi and other cells as well as capability of achieving subculture so as to provide a favorable cell culture scheme for related fundamental researches.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the technical field of cell culture, in particular to a method for isolating and culturing primary rat or mouse gastric mucosal epithelial cells. Background technique

[0002] Gastric mucosal epithelial cells have a high renewal rate, and various epithelial cells have different metabolic cycles, which are in a dynamic and delicate balance. The gastric mucosa is composed of many gastric units, and each gastric unit is composed of apical cells, parietal cells, chief cells, cervical mucous cells, pluripotent stem cells, and a small amount of endocrine cells and other epithelial cells. The apical cells, parietal cells, and chief cells are the three main types of cells in the gastric mucosa. Various epithelial cells are derived from pluripotent stem cells in the isthmus, each with different functions and turnover rates. Changes in cell differentiation, proliferation, and apoptosis directly affect the stability of gastric mucosal s...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More