Preparation method of bionic directional double-layer hydrogel for bone/cartilage repair

A cartilage repair and hydrogel technology, used in tissue regeneration, medical science, prosthesis, etc., can solve the problem of weak self-repair and regeneration ability of bone and cartilage, joint bone/cartilage damage repair, poor biological activity, weak binding force, etc. problems, to achieve the effect of inducing and promoting bone growth, promoting regeneration, and good mechanical properties

Inactive Publication Date: 2020-12-08
SOUTHWEST JIAOTONG UNIV
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Problems solved by technology

[0002] The weak self-repair and regeneration ability of bone and cartilage leads to clinical problems in the repair of joint bone/cartilage damage, while the artificial bone/cartilage transplantation treatment method is not enough due to its poor biological activity and weak binding force with bone tissue. to the ideal therapeutic effect
In addition,

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  • Preparation method of bionic directional double-layer hydrogel for bone/cartilage repair

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preparation example Construction

[0029] A method for preparing a biomimetic oriented bilayer hydrogel for bone / cartilage repair, comprising the following steps:

[0030] Step 1: Preparation of polydopamine-modified cellulose nanocrystals (PDA-CNC):

[0031] Prepare cellulose nanocrystals by acid hydrolysis, then make cellulose nanocrystals into a solution with a certain mass fraction; then add dopamine to it, keep the solution alkaline, and stir, so that dopamine self-polymerizes on the surface of cellulose nanocrystals to form polydopamine Coating, after the reaction, centrifuged and washed repeatedly, the obtained precipitate is polydopamine-modified cellulose nanocrystal (PDA-CNC);

[0032] Step 2: Preparation of cellulose nanocrystals grafted with cartilage growth factor:

[0033] Disperse the PDA-CNC prepared in step 1 in an aqueous solution to form a certain concentration of cellulose nanocrystal solution, add a certain concentration of transforming growth factor to it, and fix the growth factor on the...

Embodiment 1

[0039] A method for preparing a biomimetic oriented bilayer hydrogel for bone / cartilage repair, comprising the following steps:

[0040]Step 1: Prepare 200ml of 55wt.% sulfuric acid solution, add 4g of cellulose nanocrystalline powder at 5°C and stir evenly, then seal and stir in a water bath at 50°C for 2 hours, filter the resulting solution to pH 7, and then sonicate in an ice bath for 1 hour. The supernatant retained by centrifugation is the cellulose nanocrystal solution. According to the needs of the experiment, a 2wt.% cellulose nanocrystal solution is prepared. Take 20ml of the cellulose nanocrystal solution and add 0.2g dopamine and 1gNaOH. Stir at room temperature for 30min until the solution turns black After repeated centrifugation and washing, the lower sediment is obtained as polydopamine-modified cellulose nanocrystals (PDA-CNC);

[0041] Step 2: Disperse half of the PDA-CNC prepared in step 1 in 10ml of ionized water, add 150ng transforming growth factor (TGF-β)...

Embodiment 2

[0046] A method for preparing a biomimetic oriented bilayer hydrogel for bone / cartilage repair, comprising the following steps:

[0047] Step 1: Prepare 200ml of 55wt.% sulfuric acid solution, add 4g of cellulose nanocrystalline powder at 5°C and stir evenly, then seal and stir in a water bath at 50°C for 2 hours, filter the resulting solution to pH 7, and then sonicate in an ice bath for 1 hour. The supernatant retained by centrifugation is the cellulose nanocrystal solution. According to the needs of the experiment, a 4wt.% cellulose nanocrystal solution is prepared. Take 20ml of the cellulose nanocrystal solution and add 0.2g dopamine and 1gNaOH. Stir at room temperature for 30min until the solution turns black After repeated centrifugation and washing, the lower sediment is obtained as polydopamine-modified cellulose nanocrystals (PDA-CNC);

[0048] Step 2: Get half of the PDA-CNC prepared in step 1 and disperse it in 10ml ionized water, add 100ng transforming growth facto...

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Abstract

The invention discloses a preparation method of bionic directional double-layer hydrogel for repairing bone/cartilage, and belongs to the field of a biomaterial. The preparation method comprises the following steps of respectively preparing a growth factor modified cellulose nanocrystalline PDA-CNC for inducing cartilage regeneration and a hydroxyapatite in-situ mineralization modified cellulose nanocrystalline CaP-CNC by utilizing an adhesion effect based on mussel-imitating polydopamine; and enabling the PDA-CNC and natural macromolecules having good biocompatibility to be made into an upper-layer hydrogel prepolymer solution. Based on the directional arrangement characteristic of the upper-layer hydrogel prepolymer solution under the action of shearing force, a force field is adjusted by means of injection extrusion and the like, so that cellulose nanocrystals are arrayed in a hydrogel matrix in an oriented manner. The CaP-CNC is added into the lower-layer hydrogel, so that the mechanical strength of the lower-layer hydrogel is improved, and the hydrogel has osseointegration. By adjusting technological parameters, the upper layer and the lower layer of a hydrogel matrix have different active ingredients, structures and mechanical properties, natural cartilage/bone tissue can be simulated, and integrated repair and regeneration of cartilage and bone tissue are facilitated.

Description

technical field [0001] The invention relates to the technical field of biomaterial preparation, in particular to a method for preparing a bionic oriented bilayer hydrogel for bone / cartilage repair. Background technique [0002] The weak self-repair and regeneration ability of bone and cartilage leads to clinical problems in the repair of joint bone / cartilage damage, while the artificial bone / cartilage transplantation treatment method is not enough due to its poor biological activity and weak binding force with bone tissue. to the desired therapeutic effect. In addition, the upper layer of cartilage tissue has anisotropy, and the lower layer of bone tissue has high mechanical strength, which makes the tissue interface between cartilage and bone have obvious differences. The different characteristics of bone and cartilage make it difficult to find a material that can promote both cartilage and bone. Bone tissue growth. Therefore, it is of great significance to find a materia...

Claims

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Application Information

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IPC IPC(8): A61L27/52A61L27/54A61L27/50A61L27/58A61L27/20A61L27/18A61L27/12A61L27/22
CPCA61L27/12A61L27/18A61L27/20A61L27/227A61L27/50A61L27/52A61L27/54A61L27/58A61L2300/112A61L2300/252A61L2300/412A61L2300/414A61L2300/602A61L2430/02A61L2430/06C08L1/02C08L79/02
Inventor 胡书春刘志定鲁雄闫力维韩璐杨开明
Owner SOUTHWEST JIAOTONG UNIV
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