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Injectable cartilage repair hydrogel and preparation method thereof

A cartilage repair and hydrogel technology, which is applied in the field of biomedical engineering materials, can solve the problems of increasing patient pain, short life, metal joint allograft rejection, etc., and achieve the effect of enhancing the application

Active Publication Date: 2020-05-22
BEOGENE BIOTECH GUANGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A common allograft is to replace metal joints, but metal joints will be rejected and have a short life span of only 15 to 20 years. At that time, the interface between them and the bone will be unusable, and the patient may need to be replaced again. Great Patient's Pain
While autologous cartilage does not appear to be rejected, autografting relies on full integration and has the disadvantage of having additional defects

Method used

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  • Injectable cartilage repair hydrogel and preparation method thereof
  • Injectable cartilage repair hydrogel and preparation method thereof
  • Injectable cartilage repair hydrogel and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] An injectable cartilage repair hydrogel, the hydrogel comprising the following components in parts by weight: 10 parts of methacrylated gelatin (Gel-MA), methacrylated hyaluronic acid (HA-MA) 5 parts, 1 part of nanocellulose (CNC), 0.05 parts of melanin nanoparticles loaded with 2-([1,1-biphenyl]-4-ylcarbamoyl)benzoic acid (SMNP-KGN).

[0044] The preparation method of the injectable cartilage repair hydrogel in this example is as follows: weigh 500 μg SMNP-KGN and dissolve it in 1 mL LPBS, ultrasonically disperse it completely, then weigh 0.1 g Gel-MA, 0.05 g HA-MA and 0.01 g CNC , add the above solution, and stir in a water bath at 50°C for 1 hour, then add 0.05mL of 2% (w / v) phenyl-2,4,6-trimethylbenzoyl lithium phosphonate (LAP), and use a wavelength of A 420nm blue flashlight triggers cross-linking to obtain Gel-MA / HA-MA / CNC / SMNP-KGN hydrogel.

[0045] Among them, the preparation method of Gel-MA is: dissolve 1g of gelatin in 10mL of PBS buffer solution (pH=7.4), ...

Embodiment 2

[0049] An injectable cartilage repair hydrogel, the hydrogel comprising the following components in parts by weight: 5 parts of methacrylated gelatin (Gel-MA), methacrylated hyaluronic acid (HA-MA) 1 part, 1 part of nanocellulose (CNC), 0.01 part of melanin nanoparticles loaded with 2-([1,1-biphenyl]-4-ylcarbamoyl)benzoic acid (SMNP-KGN).

[0050] The preparation method of the injectable cartilage repair hydrogel in this example is as follows: weigh 100 μg SMNP-KGN and dissolve it in 1 mL LPBS, ultrasonically disperse it completely, then weigh 0.05 g Gel-MA, 0.01 g HA-MA and 0.01 g CNC , add the above solution, and stir in a water bath at 50°C for 1 hour, then add 0.05mL of 2% (w / v) phenyl-2,4,6-trimethylbenzoyl lithium phosphonate (LAP), and use a wavelength of A 420nm blue flashlight triggers cross-linking to obtain Gel-MA / HA-MA / CNC / SMNP-KGN hydrogel.

[0051] The preparation methods of Gel-MA, HA-MA, and SMNP-KGN are the same as in Example 1.

Embodiment 3

[0053] An injectable cartilage repair hydrogel, the hydrogel comprising the following components by weight: 15 parts of methacrylated gelatin (Gel-MA), methacrylated hyaluronic acid (HA-MA) 10 parts, 2 parts of nanocellulose (CNC), 0.05 parts of melanin nanoparticles loaded with 2-([1,1-biphenyl]-4-ylcarbamoyl)benzoic acid (SMNP-KGN).

[0054]The preparation method of the injectable cartilage repair hydrogel in this example is as follows: weigh 500 μg SMNP-KGN and dissolve it in 1 mL LPBS, disperse it completely by ultrasonic, then weigh 0.15 g Gel-MA, 0.1 g HA-MA and 0.02 g CNC , add the above solution, and stir in a water bath at 50°C for 1 hour, then add 0.05mL of 2% (w / v) phenyl-2,4,6-trimethylbenzoyl lithium phosphonate (LAP), and use a wavelength of A 420nm blue flashlight triggers cross-linking to obtain Gel-MA / HA-MA / CNC / SMNP-KGN hydrogel.

[0055] The preparation methods of Gel-MA, HA-MA, and SMNP-KGN are the same as in Example 1.

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Abstract

The invention relates to injectable cartilage repair hydrogel. The hydrogel is prepared from the following components in parts by weight: 5 to 15 parts of methacrylate gelatin, 1 to 10 parts of methacrylate hyaluronic acid, 1 to 2 parts of nano cellulose and 0.1 to 0.5 part of melanin nano particles loaded with 2-([1, 1-biphenyl]-4-ylcarbamyl) benzoic acid. According to the hydrogel disclosed by the invention, a catechol functionalized network is used as a paramagnetic metal ion chelating bracket, and the synthesized melanin base material has excellent biocompatibility and the capability of coordinating isolated paramagnetic metal centers. The base material is loaded with a cartilage repair promoting small molecular compound 2-([1, 1-biphenyl]-4-ylcarbamyl) benzoic acid. The nanoparticlesare dispersed into injectable hydrogel, so that the product can remarkably induce bone marrow mesenchymal stem cells to be differentiated into cartilage, has an extremely strong cartilage formation promoting effect, realizes detection of cartilage repair conditions in MRI, photoacoustic and photo-thermal multiple imaging modes, and enhancing application in medical clinics.

Description

technical field [0001] The invention relates to an injectable cartilage repair hydrogel and a preparation method thereof, belonging to the field of biomedical engineering materials. Background technique [0002] In recent years, with the accelerated aging of the population, more and more people are troubled by articular cartilage damage. Articular cartilage may be bruised during exercise or mechanically injured by accidents such as car accidents, causing the articular cartilage to break and fall. Since cartilage itself is very poorly self-repairing, a wallless and avascular tissue, its regeneration is often compromised by its inherent lack of adequate healing response. Once damaged, it cannot recover by itself, and gradually wears out. When the bone under the cartilage is corroded, pain and swelling will occur, which limits the patient's ability to move. If the articular cartilage is too damaged to be used, the only option is a total knee replacement, in which the damaged ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20A61L27/22A61L27/50A61L27/52A61L27/54
CPCA61L27/222A61L27/20A61L27/54A61L27/52A61L27/50A61L2400/06A61L2430/06A61L2300/214A61L2300/412A61L2300/442C08L5/08C08L1/04
Inventor 蓝咏刘玉李芸冯龙宝
Owner BEOGENE BIOTECH GUANGZHOU
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