Parasitic protozoon lyophilized powder and preparation method thereof

A technology for freeze-dried powder and protozoa, which is applied in the fields of cryogenic biology and biomedicine, and achieves the effects of easy long-term storage, short drying time and stable properties.

Inactive Publication Date: 2013-09-18
SUN YAT SEN UNIV
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology helps prevent frost damaging and deteriorating microorganisms during their production processes. It also improves upon crystal structure after lyophilization without adding any extra steps like heat treatment. Additionally it allows for easy handling and storing these products over longer periods while maintaining stability even under harsh conditions such as high temperatures.

Problems solved by technology

This patented technical solution describes methods for storing biovaporers' products such as bovidonics while maintaining good stability over a wide range of temperatures. Current methods involve freezer-dewage techniques involving expensive equipment like liquid nitrate/water mixtures, but these have limitations including poor shelf life span, difficulty handling large quantities of samples, potential harmful impact on living subjects when exposed to extreme environments, and difficulties associated with safely disposal of waste containing residual liquids.

Method used

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  • Parasitic protozoon lyophilized powder and preparation method thereof

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Embodiment 1

[0026] Weigh trehalose and tea polyphenols, dissolve them in PBS buffer, and prepare a freeze-drying protectant solution. The concentration of trehalose is 0.4 mol / L, and the concentration of tea polyphenols is 0.2 mol / L. Store in a refrigerator at 4°C for later use. in. Arterial blood was collected from the heart of mice infected with Trypanosoma brucei for later use. The mouse blood containing trypanosomiasis and the lyoprotectant solution were fully mixed at a volume ratio of 1:1 to make a trypanosomiasis suspension, which was divided into 2.5ml vials with a volume of 0.2ml of trypanosomiasis suspension. After incubating at room temperature for 5 minutes, put them directly into liquid nitrogen for pre-freezing. After 24 hours, the pre-frozen trypanosome suspension was transferred together with the vial to a freeze dryer for vacuum drying to finally obtain a freeze-dried powder of parasitic protozoa. The vacuum drying conditions are as follows: drying pressure 10 Pa, separ...

Embodiment 2

[0028] Weigh trehalose and tea polyphenols, dissolve them in PBS buffer, and prepare a lyoprotectant solution with a trehalose concentration of 0.1 mol / L, and store them in a 4°C refrigerator for later use. Arterial blood was collected from the heart of mice infected with Plasmodium and collected for later use. Mix the blood of mice containing malaria parasites and the lyoprotectant solution at a volume ratio of 1:1 to make a trypanosomal suspension, and distribute them in 2.5ml vials with a volume of 0.2ml of malaria parasite suspension. After incubating at room temperature for 10 minutes, put them directly into liquid nitrogen for pre-freezing. After 24 hours, transfer the pre-frozen malaria parasite suspension together with the vial to a freeze dryer for vacuum drying to finally obtain a parasite freeze-dried powder. The vacuum drying conditions are as follows: drying pressure 20 Pa, separator temperature -30°C, cold trap temperature -180°C, and drying time 100 minutes. B...

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Abstract

The invention discloses a preparation method of a parasitic protozoon lyophilized powder. The preparation method comprises the following steps: (1) uniformly dispersing parasitic protozoa into a freeze-drying protective additive to form a parasitic protozoon suspension liquid, separately filling the parasitic protozoon suspension liquid into a penicillin bottle, incubating at room temperature, and putting the penicillin bottle into liquid nitrogen to be prefrozen; and (2) transferring the prefrozen parasitic protozoon suspension liquid into a freeze dryer for vacuum drying so as to obtain the parasitic protozoon lyophilized powder. A direct liquid nitrogen input mode is adopted to quickly vitrify and prefreeze the parasitic protozoa, so that freezing injury and drying stress injury can be effectively relieved, and the rehydration recovery of the parasitic protozoon lyophilized powder is greatly increased; series optimized technological parameters are adopted for vacuum drying, the drying time is short and the operation is simple; the parasitic protozoon lyophilized powder has the advantages of low water content, stable performance, convenience in long-term storage at room temperature and remote transportation. The obtained parasitic protozoon lyophilized powder can be applied to scientific research or biological vaccine development.

Description

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Claims

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Application Information

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Owner SUN YAT SEN UNIV
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