Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of stem cell cryopreservation liquid and its preparation method and cryopreservation method

A technology for cryopreserving liquid and quality stem cells, which is applied in the field of stem cell cryopreservation liquid and its preparation, which can solve the problems of serum pollution and inability to meet the clinical use requirements of stem cell therapy, and reduce negative effects, prevent the risk of pollution and allergens, High safety effect

Active Publication Date: 2020-11-10
广州赛隽生物科技有限公司
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the addition of serum has the risk of introducing contamination and allergens, which cannot meet the clinical needs of stem cell therapy

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of stem cell cryopreservation liquid and its preparation method and cryopreservation method
  • A kind of stem cell cryopreservation liquid and its preparation method and cryopreservation method
  • A kind of stem cell cryopreservation liquid and its preparation method and cryopreservation method

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0042] The present invention also provides a preparation method of Plasma-Lyte A electrolyte injection, which comprises the following steps:

[0043] (1) Use an electronic balance to accurately weigh 5.26g of USP grade sodium chloride (NaCl), 0.37g of USP grade potassium chloride (KCl), and 0.37g of USP grade magnesium chloride hexahydrate (MgCl). 2 ·6H 2 O) 0.3g, pharmacopoeia grade sodium gluconate (C 6 h 11 NaO 7 )5.02g, pharmacopoeia grade sodium acetate trihydrate (C 2 h 3 NaO 2 ·3H 2 O) 3.68g;

[0044] (2) Add the auxiliary materials weighed in step (1) into a 1L beaker in turn, add 900mL of ultrapure water into the beaker, fully dissolve, and configure it into an aqueous solution;

[0045] (3) Measure the pH of the aqueous solution obtained in step (2) with a pH meter, and adjust the pH of the aqueous solution to 7.2 to 7.4, and finally set the volume to 1 L;

[0046] (4) Divide the aqueous solution obtained in step (3) into several 500mL blue cap reagent bottl...

Embodiment 1

[0056] A stem cell cryopreservation liquid, which is composed of the following components by weight: 1 part of trehalose, 0.2 parts of dextran 40, 3 parts of human serum albumin, 3 parts of dimethyl sulfoxide, 5 parts of hydroxyethyl starch and Maili A injection 87.8 parts.

Embodiment 2

[0058] A stem cell cryopreservation liquid, which is composed of the following components by weight: 1 part of trehalose, 0.5 parts of dextran 40, 5 parts of human albumin, 5 parts of dimethyl sulfoxide, 6 parts of hydroxyethyl starch and Maili A injection 82.5 parts.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a stem cell freezing and storing medium and a preparation method and freezing and storing method thereof. The stem cell freezing and storing medium comprises, by weight, 3-10 parts of dimethyl sulfoxide, 2-7 parts of human serum albumin, 0.5-3 parts of mycose, 0.2-2 parts of dextran 40 and 2-6 parts of hetastarch. Cells can be frozen for a long time by the stem cell freezing and storing medium, freezing damage of the cells can be remarkably reduced, the resuscitated cells have a high degree of survival rate and adherent property, and freezing and storing effect of the cells is improved; meanwhile, the compositions of the stem cell freezing and storing medium are clear and are medical compendial injection-grade excipients without containing serum, the risk of contamination and allergen introduced by the use of heterogeneous serum is effectively prevented, the content of DMSO (dimethylsulfoxide) is low, the negative effect of the DMSO on the cells is lowered, highsecurity and good stability are achieved, requirements of CFDA (China food and drug administration) and FDA (food and drug administration) are met, and the stem cell freezing and storing medium can be directly used in human infusion and is suitable for clinical research and treatment.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a stem cell cryopreservation solution, a preparation method and a cryopreservation method thereof. Background technique [0002] Stem cells are a type of cells with self-renewal and multi-directional differentiation potential, which have been widely used in scientific research and clinical treatment. Stem cells have broad application prospects in cell therapy, regenerative medicine, drug discovery, toxicology, and developmental biology. At present, there are stem cell drug products on the market all over the world. With the development of science and technology, stem cells will be more effectively and widely used in the treatment of various diseases to make up for the ineffectiveness of traditional drug treatments for certain diseases. [0003] During the preparation or clinical application of stem cells, the main storage method is to store them in liquid nitrogen at low temperature...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 颜孙兴王文成黄梅连黄伟英
Owner 广州赛隽生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com