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A kind of preparation method of fish protein peptide

A technology of fish protein and fish paste, applied in the biological field, can solve the problems of high hydrolysis efficiency, high cost, and long enzymatic hydrolysis time of small peptides, etc., to improve flavor and nutritional value, increase production, cheap and easy to obtain Effect

Active Publication Date: 2021-04-06
NINGBO HAIZHIYUAN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are many methods for preparing bioactive peptides, the common ones are enzymatic hydrolysis and microbial methods, but due to the high cost of external enzymes in the enzymatic hydrolysis method, the enzymatic hydrolysis time for preparing small peptides by the microbial method is too long, the cost is high, and conventional methods are used. After protease enzymatic hydrolysis or microbial hydrolysis, the amount of protein hydrolyzed in raw materials can only reach 26%, and the content of polypeptides below 3000Da is only 83%. This hydrolysis efficiency and degree of hydrolysis are difficult to achieve industrial production.

Method used

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  • A kind of preparation method of fish protein peptide
  • A kind of preparation method of fish protein peptide

Examples

Experimental program
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Effect test

Embodiment 1

[0047] The preparation method of a kind of fish protein peptide in embodiment 1 is basically as attached figure 1 As shown, the specific steps are as follows:

[0048] Raw material preparation steps: Prepare raw materials, raw materials include marine low-value small miscellaneous fish and aquatic product scraps, among which marine low-value small miscellaneous fish mainly include anchovy, shrimp, plucked headfish, crane needle fish and sea dragon fish; aquatic products Offals include fish heads, skins, tails and viscera;

[0049] Crushing step: mix the frozen low-value marine fish and aquatic product scraps with water, and the mass ratio of raw materials to water is 1:2, thaw the raw materials with water, and remove them after they are thawed and dispersed; The final raw material is put into the pulverizer, and the pulverizer is started to pulverize to form fish paste, and the mesh number of the fish paste is less than or equal to 10, and then the fish paste is placed in the...

Embodiment 2

[0058] The difference between embodiment 2 and embodiment 1 is that, as figure 2 As shown, in the microbial fermentation step, the fermentation time used is 36h. After this period of microbial fermentation and hydrolysis, the mass of fish protein peptides obtained from the hydrolysis of raw materials is 31% of the total mass of raw materials. Compared with Example 1 The degree of hydrolysis of raw materials has been improved.

Embodiment 3

[0060] The difference between embodiment 3 and embodiment 1 is that, as figure 2 As shown, in the microbial fermentation step, the fermentation time used is 30h. After this period of microbial fermentation and hydrolysis, the mass of fish protein peptides obtained from the hydrolysis of raw materials is 32% of the total mass of raw materials. Compared with Example 1 The degree of hydrolysis of raw materials has been improved.

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Abstract

The invention relates to the field of biotechnology, and specifically discloses a method for preparing fish protein peptides, including a raw material preparation step, the raw material includes marine low-value small miscellaneous fish and aquatic product leftovers; a raw material crushing step; a fish paste enzymatic hydrolysis step: Transfer the fish paste in the enzymolysis tank to the enzymolysis tank, add water to the enzymolysis tank, the mass ratio of water and fish paste is 1:1, mix the water and fish paste to form a mixture A, and the enzymolysis temperature is 50-70°C, The pH during enzymolysis is 7.0, and the enzymolysis time is 2-6h; the step of inactivating the enzyme; the step of preparing the small peptide solution; the step of spray drying the enzymolysis solution; the step of microbial fermentation: mixing rice into the solid matter in the step of preparing the small peptide solution Aspergillus, forming a mixture C, the moisture content in the mixture C is 30-60%, the temperature is 25-45°C, and the fermentation time is 16-28h, forming a mixture D; a drying step; a mixing and crushing step. This scheme can extract protein peptides from low-value small miscellaneous fish and aquatic product waste, thereby reducing the processing cost of protein peptides.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for preparing fish protein peptides. Background technique [0002] Due to the rapid development of aquaculture and special breeding in China, the demand for animal protein feed is growing rapidly. Animal protein feed has the advantages of high protein content, high animal meat yield, and easy matching with other ingredients. However, the fish meal produced in my country is generally high-grade. The price of fishmeal is relatively low, and the processing technology is backward, resulting in the low price of fishmeal. Some fishmeal with high protein content, such as special meal for prawns and special meal for soft-shelled turtle, have a serious problem of relying on imports. [0003] At present, the main economic fish resources in the ocean are withered, and the low-value small trash fish are insufficiently caught and processed due to their various shortcomings. L...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C12P21/00C07K1/34C07K1/14
CPCC07K1/14C07K1/34C12P21/00C12P21/06
Inventor 叶晨
Owner NINGBO HAIZHIYUAN BIOTECH CO LTD
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