A method for ethanol production by semi-synchronous enzymatic hydrolysis and fermentation of defatted peanut powder prehydrolyzate and pretreated solid substrate

A technology based on defatted peanuts and solid substrates, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of increasing the production cost of lignocellulosic fuel ethanol, increasing the steps of pretreatment, etc., and achieve the promotion of commercialization Promotion, good biocompatibility, effect of reducing energy consumption

Active Publication Date: 2021-08-10
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, although fed-batch can partially overcome the adverse effects of mass and heat transfer, the patent still deals with the adverse effects of lignin
In order to overcome the ineffective adsorption of lignin to cellulase, after steam explosion pretreatment, the patent further uses alkaline hydrogen peroxide post-treatment to further remove lignin from the pretreated solid matrix. Although a certain effect has been achieved, it increases pretreatment step, and new effluent
In addition, alkaline hydrogen peroxide post-treatment cannot completely remove the lignin in the pre-treated solid substrate. In order to improve the efficiency of subsequent enzymatic hydrolysis and fermentation, the amount of enzyme added in this patent is relatively high. When the ethanol concentration reaches 65g / L When above, the dosage of cellulase reaches more than 30 FPU / g dextran, which will significantly increase the overall production cost of lignocellulosic fuel ethanol

Method used

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  • A method for ethanol production by semi-synchronous enzymatic hydrolysis and fermentation of defatted peanut powder prehydrolyzate and pretreated solid substrate
  • A method for ethanol production by semi-synchronous enzymatic hydrolysis and fermentation of defatted peanut powder prehydrolyzate and pretreated solid substrate
  • A method for ethanol production by semi-synchronous enzymatic hydrolysis and fermentation of defatted peanut powder prehydrolyzate and pretreated solid substrate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] (1) Preparation of bamboo pretreatment solid matrix:

[0051] First, 10g of bamboo powder passed through a 40-mesh sieve is added to a microwave reaction tank containing a polytetrafluoroethylene lining, and an aqueous solution of 150g of benzenesulfonic acid is added. The mass percentage of benzenesulfonic acid in the pretreatment liquid is 80%, and the microwave oven is sealed. After the reaction tank was treated at 95 ° C for 30 min, after the pretreatment was completed, the microwave reaction tank was taken out and cooled to room temperature in cold water, and then solid-liquid separation was carried out in a Buchner funnel with filter paper; then, the pretreatment was cleaned with deionized water. The solid matrix is ​​treated until the pH value of the filtrate is in the range of 6.8-7.0, and the washed pretreated solid matrix is ​​used for subsequent semi-synchronous enzymatic hydrolysis and fermentation.

[0052] (2) Prehydrolysis of defatted peanut powder:

[0...

Embodiment 2～6

[0073] Embodiment 2~6 and comparative example 2~4

[0074] The specific schemes of Examples 2~6 and Comparative Examples 2~4 and the results of ethanol fermentation are shown in Table 1 and Table 2 respectively:

[0075] Woody biomass raw materials are all bamboo powder or eucalyptus powder over 40 meshes; HC, CL, CB, G and YC are the abbreviations of hemicellulase, cellulase, cellobiase, dextran and yeast cells, respectively; R is the abbreviation of pretreatment solid-liquid ratio; AASA is the abbreviation of acetic acid-sodium acetate; DPF is the abbreviation of defatted peanut flour; PS is the abbreviation of pretreatment solid matrix.

[0076] For the pretreatment of benzenesulfonic acid, hot water, dilute sulfuric acid or dilute NaOH, after the pretreatment is completed, use a Buchner funnel with filter paper for solid-liquid separation, and wash the pretreated solid matrix with deionized water until the pH of the filtrate is 6.8 In the range of ~7.0, the washed pretrea...

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Abstract

The invention discloses a method for producing ethanol by semi-synchronous enzymatic hydrolysis and fermentation of defatted peanut powder prehydrolyzate and pretreated solid substrate. The method firstly performs prehydrolysis on defatted peanut powder, including ball milling treatment, enzymatic hydrolysis, separation and other processes; Finally, the defatted peanut powder prehydrolyzate was mixed with the pretreated solid substrate and ultrasonically treated; finally, after adding the pretreated solid substrate, cellulase, cellobiase and yeast in batches, the defatted peanut powder prehydrolyzate Semi-synchronous enzymolysis and fermentation with pretreated solid substrate to produce fuel ethanol. The present invention extracts peanut protein, monosaccharides or oligosaccharides from cheap defatted peanut powder. Peanut protein can effectively shield the lignin deposited on the surface of the pretreated solid substrate and reduce the amount of cellulase. The sugar can increase the concentration of fermentable sugar, increase the concentration of fuel ethanol in the fermentation broth, and achieve the purpose of effectively reducing the production cost of fuel ethanol.

Description

technical field [0001] The invention belongs to the technical field of new energy, and in particular relates to a method for producing ethanol by semi-synchronous enzymatic hydrolysis and fermentation of defatted peanut powder prehydrolyzate and pretreated solid substrate. Background technique [0002] In order to reduce the rapid consumption of fossil-based energy and its harm to social and economic stability and the environment, countries around the world are gradually seeking sustainable alternative energy. In addition to biogasoline and biodiesel, fuel ethanol can be mixed with fossil-based gasoline (E10 and E85 blended fuel, etc.) as a transportation fuel, which has attracted widespread attention worldwide. [0003] Woody biomass has the advantages of sustainable utilization, wide distribution, large yield, and rich carbohydrates. It has been regarded as an ideal raw material for the production of fuel ethanol by researchers around the world. However, the structure of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/10C12R1/645
CPCC12P7/10C12P2201/00Y02E50/10
Inventor 刘婧罗小林杨光绪龚正刚黄六莲陈礼辉
Owner FUJIAN AGRI & FORESTRY UNIV
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