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Method for extracting total flavonoid aglycone from Scutellaria baicalensis based on infrared-assisted enzymatic hydrolysis technology

A technology for total flavonoid aglycone and extraction method, which is applied in the field of extraction of total flavonoid aglycone from Scutellaria baicalensis based on infrared-assisted self-enzymatic hydrolysis technology, can solve the problems of cumbersome operation, low yield, long time consumption and the like, and achieves sufficient enzymatic hydrolysis and operation. simple effect

Inactive Publication Date: 2018-03-27
FUDAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, the extraction methods of total flavonoid aglycones of Scutellaria baicalensis often use external enzyme extraction, self-enzyme water bath extraction, etc., but these methods often have the disadvantages of cumbersome operation, long time-consuming or low yield.
So far, there has been no report on the extraction method that combines infrared assistance with enzymatic hydrolysis of Scutellaria baicalensis

Method used

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  • Method for extracting total flavonoid aglycone from Scutellaria baicalensis based on infrared-assisted enzymatic hydrolysis technology
  • Method for extracting total flavonoid aglycone from Scutellaria baicalensis based on infrared-assisted enzymatic hydrolysis technology
  • Method for extracting total flavonoid aglycone from Scutellaria baicalensis based on infrared-assisted enzymatic hydrolysis technology

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Embodiment Construction

[0024] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments.

[0025] 1. Instruments and reagents

[0026] Instruments: Agilent 1200 high-performance liquid chromatography, Anderson hard infrared lamp, and infrared auxiliary extraction device made in the laboratory.

[0027] Test drugs: baicalein reference substance (97.8%, China National Institute for the Control of Pharmaceutical and Biological Products), wogonin reference substance (98%, Wokai), kelp A reference substance (98%, Nanjing Zelang Pharmaceutical Technology Co., Ltd. ); phosphoric acid, hydrochloric acid, citric acid, disodium hydrogen phosphate (analytical grade, Sinopharm Chemical Reagent Co., Ltd.).

[0028] Methanol (chromatographically pure, Merck Company, USA), pure water is Millipore deionized water.

[0029] 2. Test methods and results

[0030] 2.1 Chromatographic conditions:

[0031] Using Welch Ultimate LP-C18 (250×4.6mm, 5μm) ...

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Abstract

The invention relates to a method for extracting total flavonoid aglycone from Scutellaria baicalensis based on infrared-assisted enzymatic hydrolysis technology, belonging to the field of analyticalchemistry. The method comprises the following steps: selecting the medicinal powder of Scutellaria baicalensis, and carrying out infrared-assisted enzymatic hydrolysis at first, i.e., adding water ora buffer solution with a certain pH value as an enzymatic hydrolysis solution, subjecting the medicinal powder of Scutellaria baicalensis to infrared radiation with a certain power at a certain temperature for a certain period of time, and then adding an organic solvent for infrared-assisted extraction; and then subjecting the obtained extract to filtering at first and then to detection and analysis with a high-peformance liquid chromatograph. The infrared-assisted enzymatic hydrolysis technology provided by the invention is sufficient in enzymatic hydrolysis, fast in speed, high in efficiency, safe, friendly to environment, and beneficial for extraction of the total flavonoid aglycone of Scutellaria baicalensis in the medicinal material Scutellaria baicalensis; and the method is of greatsignificance to development and utilization of the medicinal material Scutellaria baicalensis and to preparation of the total flavonoid aglycone of Scutellaria baicalensis.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and relates to a method for extracting total flavonoid aglycone of Scutellaria baicalensis, in particular to a method for extracting total flavonoid aglycone of Scutellaria baicalensis based on an infrared-assisted self-enzymolysis technology. Background technique [0002] The prior art discloses that Scutellaria baicalensis is the dry root of Scutellaria baicalensis Georgi., which was first published in "Shen Nong's Herbal Classic". Scutellaria baicalensis has the effects of clearing away heat and dampness, purging fire and detoxification, stopping bleeding and preventing miscarriage. Modern pharmacology shows that Scutellaria baicalensis has antibacterial, anti-viral, anti-inflammatory, anti-oxidant, anti-tumor, immune regulation, antipyretic and analgesic effects, protects the liver and promotes gallbladder, etc., and its main active ingredients are flavonoids, including baicalin, Scutellar...

Claims

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Application Information

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IPC IPC(8): A61K36/539
CPCA61K36/539A61K2236/19A61K2236/333
Inventor 段更利郁颖佳王利苹段昊天龙佳坤
Owner FUDAN UNIV
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