36results about How to "Fast enzymatic hydrolysis" patented technology

The invention discloses a preparation method of phosphorylated peptidoglycan. The method is characterized by comprising the following steps of: inoculating Lactobacillus acidophilus on a culture medium for cultivation, centrifuging to collect thallus, repeatedly washing the thallus, suspending the thallus in hydrofluoric acid solution for one night at 4 DEG C, centrifugally collecting cell wall sediment, and washing the sediment to become neutral; dissolving the sediment into Trypsin chymotrypsin phosphate buffer solution, inactivating in boiling water, centrifuging the liquid supernatant, washing the sediment; dissolving the sediment in diethyl ether, stirring and centrifuging to collect sediment, and washing the sediment with water until completely removing the smell of diethyl ether, then obtaining the peptidoglycan; dissolving the peptidoglycan in Lysostaphin phosphate buffer solution for performing enzymolysis and centrifuging, taking the liquid supernatant, centrifuging the liquid supernatant, and centrifugally washing the sediment with water to get micromolecule peptidoglycan; and finally, dissolving the micromolecule peptidoglycan in mixed phosphate solution, precipitating with alcohol after reaction, treating the alcohol precipitated peptidoglycan by freeze drying and redissolving, and adding the redissolved solution in a dialysis bag, performing dialysis concentration, and then, performing freeze drying to get the phosphorylated peptidoglycan. The method has the advantages of increasing the solubility remarkably and improving the immunological effect to a certain extent.

The invention discloses a method for preparing an amino acid aqueous solution by using dead chickens. The method comprises the following steps: mincing and heating: putting the dead chickens into a mincing device for mincing, adding water and proteolytic enzyme into the mincing device, and heating the mincing device; stirring: adding the minced dead chicken, water and a proteolytic enzyme mixtureinto a stirring device for stirring, adding a compound enzyme into the stirring device, and continuously heating the mixture; enzyme deactivation: putting the treated mixture into an enzyme deactivation tank for high-temperature enzyme deactivation treatment; and filtering: filtering the treated mixture, dividing the filtered mixture into filter residues and an amino acid aqueous solution, feedingthe filter residues back into the stirring device, mixing the filter residues with the newly added mixture, treating again, and collecting the separated amino acid aqueous solution for later use. Themethod has characteristics of simple operation, rapid enzymolysis, high amino acid content, significant environmental benefit and significant economic benefit.

Provided is a method for preparing chitosan oligosaccharide by using a lipase. The method comprises dissolving chitosan in an acetic acid-sodium acetate buffer solution and adding sucrose fatty acid ester to prepare a chitosan solution, adding a lipase to the chitosan solution for enzymatic hydrolysis, dialyzing an enzymatic hydrolysate after the enzymatic hydrolysis, and concentrating the resulting dialysate and drying.

The invention relates to the technical field of biotechnology and biochemical detection, and especially relates to a beta-agarose, and anapplication thereof in quantitative detection of agar. The enzyme has a novel amino acid sequence and good enzymatic properties, and the amino acid sequence of the enzyme is represented by SEQ ID NO. 1. The invention discloses a kit for detecting the agar contentby using beta-agarose Aga16_WA. The kit comprises a reagent A, a reagent B, a reagent C and a calibrator, wherein the reagent A comprises the beta-agarose Aga16_WA and a stabilizer. The kit is good in linear range and high in accuracy, and can be promoted and used in the market.

The invention relates to the technical field of biotechnology and biochemical detection, in particular to a method for quantitatively detecting kappa-carrageenan by an enzymic method. The method comprises the following steps: specifically hydrolyzing the kappa-carrageenan into reducing sugar by utilizing kappa-carrageenase; after enzyme inactivation, adding a p-hydroxybenzohydrazide solution for achromogenic reaction; after centrifugation, measuring a light absorption value of supernate at the position of 400-420 nm; and the comparing the light absorption value to a standard curve to obtain the content of the kappa-carrageenan. The detection method is good in linear range and high in accuracy, and can be popularized and used in the markets.

The invention discloses an enzymatic hydrolysis method of protein and glycoprotein. The enzymatic hydrolysis method of protein or glycoprotein disclosed in the invention comprises: incubating the protein or glycoprotein and protease in a reaction system for 0.5-15 minutes to complete the enzymatic hydrolysis of the protein or glycoprotein; the reaction system contains NH 4 HCO 3 , NH in the reaction system 4 HCO 3 The concentration of the protein is greater than 0mM and less than or equal to 100mM; the mass ratio of protein or glycoprotein to protease in the reaction system is 1:(0.0001-3); the incubation time is 0.5-10 minutes, and the temperature is 4-80°C. The method of the present invention can be applied to fields that require protein enzymatic hydrolysis: such as identification of proteins, obtaining specific glycopeptides, identifying peptide segments with mutated amino acids, and the like.

The invention relates to the technical field of biotechnology and biochemical detection, in particular to a β-agarase and its application in agar quantitative detection. The enzyme has a novel amino acid sequence and good enzymatic properties, and its amino acid sequence is SEQ ID NO.1. The present invention develops a kit for detecting agar content by using β-agarase Aga16_WA: including reagent A, reagent B, reagent C and calibrator, wherein reagent A contains β-agarase Aga16_WA and a stabilizer. The kit has good linear range and high accuracy, and can be popularized and used in the market.

The invention relates to a method for producing fibrin polypeptide powder (pig blood fibrin polypeptide powder) via hydrolyzing blood clots of pig blood. In the processes of collecting, storing and processing pig blood, the principal component of the blood clots generated via blood clotting is fibrin; and separation and cutting, enzymolysis via proteinase preparation, enzyme deactivation, filtration and drying via atomizing are carried out on the blood clots, thus, the pig blood fibrin polypeptide powder is obtained. By means of the technical scheme provided by the invention, fibrin polypeptide is produced via the enzymolysis of the blood clots; when the fibrin polypeptide is used as a feed additive, the pollution is reduced and the green environmental protection requirements are met; andwhen the fibrin polypeptide is used as an excellent animal protein source, the digestibility of the protein is improved and the trend of the upgrading of the current enterprise technologies is met.

The invention discloses a method for preparing galactomannose oligosaccharide enzymatic hydrolysis solution from a guar gum solution. First, a citric acid-sodium citrate buffer solution is used to prepare a guar gum solution, then the temperature and time are controlled for heating and swelling, and after cooling, the guar gum solution is prepared. At the same time, three enzymes, β-mannanase, xylanase, and endoglucanase, were added, mixed thoroughly, and the enzymatic hydrolysis temperature and time were controlled for degradation. The invention realizes the rapid enzymatic hydrolysis of guar gum under high concentration, obtains the galactomannose oligosaccharide enzymatic hydrolysis solution, and saves the cost of drying and purification in the subsequent production of galactomannose oligosaccharide.

The invention discloses a method for rapidly preparing an enzymatic hydrolysis solution of oligogalactomannose by using guar gum. Firstly, a guar gum solution is prepared with a citric acid-sodium citrate buffer solution, and then the temperature and time are controlled to heat and swell, and then cooled. Finally, add β-mannanase, xylanase, and cellulase at the same time, mix well, and control the enzymatic hydrolysis temperature and time for degradation. The invention realizes the rapid enzymolysis of guar gum at high concentration, obtains the enzymatic hydrolysis solution of galactomannose oligosaccharides, and saves the cost of drying and purification during the subsequent production of galactomannose oligosaccharides.

The invention discloses an enzyme membrane coupled enzymolysis device for efficiently extracting heparin sodium. The enzyme membrane coupled enzymolysis device comprises a loading box, an operation box I, an operation box II and a collection box, wherein a centrifugal machine is arranged at the upper end of the loading box, the loading box, the operation box I, the operation box II and the collection box are sequentially connected by virtue of transmission pipes from left to right, a pH controller is arranged inside the operation box I, a protease container and a sterilizer are arranged at the upper end of the operation box II, the protease container and the sterilizer are connected by virtue of a transmission pipe, a high temperature steam engine is arranged inside the operation box II, a vacuum pump is arranged inside the collection box, a temperature controller is arranged at the upper end of the collection box, heparin sodium is obtained and transmitted to the interior of the collection box, the temperature controller is utilized for controlling temperature of the collection box, the vacuum pump is used for pumping the air, an appropriate environment is constructed for extraction of heparin sodium, and design is novel, so that the enzyme membrane coupled enzymolysis device is worthy of popularization.

The invention provides a preparation method of a sinonovacula constricta anti-hypertension peptide. The method comprises the following operation steps: (1) enzymolysis; (2) ultrafiltration; and (3) preparation of the sinonovacula constricta anti-hypertension peptide. The method has the following beneficial effects: sinonovacula constricta is subjected to enzymolysis by adopting a complex enzyme preparation, so that the anti-hypertension peptide can be obtained, the activity of the anti-hypertension peptide is not destroyed, the degradation speed is high and the reaction time is shortened; the solubility of the anti-hypertension peptide in a Tris-HCl buffer solution can be improved by adding a cosolvent and the extraction rate of the anti-hypertension peptide is improved; a hydration film around molecules of the anti-hypertension peptide can be weakened or disappear by a precipitation agent and the precipitation rate is high; the content of precipitated impure protein and polypeptide is low; the prepared sinonovacula constricta anti-hypertension peptide is high in purity, high in activity and good in anti-hypertension effect; and the sinonovacula constricta anti-hypertension peptide prepared by using an enzymolysis technology is green and safe and can be securely used for production of anti-hypertension drugs.

The invention relates to a sample pretreatment device for improving protein analysis speed, which combines protein collection and enzymatic hydrolysis functions. The invention relates to a preparation method thereof. The sample pretreatment device comprises a centrifuge tube with an upper cover, which is characterized in that the middle part of the upper cover of the centrifugal tube is provided with a hole, the inner wall of the centrifugal tube is attached with an annular hollow porous rigid integral substrate layer whose surface is fixed with enzymes via active groups modifying the substrate. The sample pretreatment device has the advantages of large sample processing amount, fast protein enzymatic hydrolysis speed, simple manufacture method and low cost, which can be used for fast enzymatic hydrolysis of collected protein samples. The invention has high practical value for the research of high flux protein.

The invention relates to the technical field of protein extraction. In order to solve the problems of a large number of by-products and low resource utilization in the processing of Japanese croaker, a dipeptidyl peptidase-IV inhibitory peptide derived from collagen in the swim bladder of Japanese croaker is provided. The preparation method comprises the following steps: (1) pretreating the swim bladder of Japanese yellow croaker, then extracting collagen with 5wt% acetic acid combined with pepsin, and centrifugally drying to obtain the swim bladder collagen of Japanese yellow croaker; (2) using compound The enzymatic hydrolysis of the Japanese croaker swim bladder collagen obtained in step (1) to obtain the Japanese croaker swim bladder collagen peptide; (3) combining the Japanese croaker swim bladder collagen peptide with a dipeptidyl peptidase screening kit, namely Obtain dipeptidyl peptidase-IV inhibitory peptide. In the present invention, the dipeptidyl peptidase-IV inhibitory peptide is prepared by using the swim bladder of Japanese yellow croaker as the main raw material through a relatively efficient process. antihyperglycemic effect.

The invention discloses kelp microcrystalline cellulose and a preparation method thereof as well as application of the prepared microcrystalline cellulose. The preparation method of the kelp microcrystalline cellulose comprises the following steps of by adopting waste kelp residues after kelp glue-extracting as the materials, carrying out flocculation collecting, acidification decalcification, alkaline treatment, bleaching treatment, washing, drying and hydrolytic treatment, wherein in the hydrolytic treatment, two different low-temperature cellulases are respectively added according to the addition of 20U / g-30U / g; carrying out enzymolysis for 0.5-12 hours in a water bath of 10 DEG C-40 DEG C; and drying and crushing to obtain the kelp microcrystalline cellulose. According to the preparation method of the kelp microcrystalline cellulose, the production cost is low, the environment pollution is small, the hydrolysis step adopts a double-enzyme jointed enzymolysis technology, the degradation speed is stable, the degraded cellulose is uniform in molecular weight, the cellulose crystalline grains are small and uniform, degradability of the fibers can be effectively controlled, and the microcrystalline cellulose with high purity and high activity is obtained. The kelp microcrystalline cellulose disclosed by the invention is high in cellulose content, high in water holding capacity and expansion force, and can be used for food additive and pharmaceutical adjuvant in the food industry.

The invention aims to the field of chemical engineering, and relates to a method for preparing L-methionine. According to the method, acetylated saponification liquid is used as production raw material, acid is added into the raw material, the pH is adjusted to be acidic, after reduced pressure distillation, an organic solvent is used for treatment, so that a solution containing D,L-acetyl methionine is obtained, then the organic solvent is removed to obtain D,L-acetyl methionine crystals, and a resolution reaction is performed on the produced D,L-acetyl methionine crystals, so that the L-methionine is obtained; the technology does not need refined D,L-methionine, the soponification liquid for producing the D,L-methionine is used for performing an acetylated reaction, and therefore production cost is reduced; after the acetylation, water is evaporated to dryness, the organic solvent is used for desalting, the yield of the D,L-acetyl methionine reaches 97-99%, and the purity of the D,L-acetyl methionine reaches above 96%; the enzymolysis speed of the D,L-acetyl methionine is higher than that of a traditional method, the obtained L-methionine is good in crystal form and whiteness, the purity reaches above 99.5%, and the total yield of the L-methionine is improved to 78%; according to the technology, a circulation technology is integrated, the design is reasonable, and production cost is reduced.

The invention relates to a method for refining sheep tail oil by a biological enzyme method. The method comprises the steps of (1) pretreating raw materials; (2) preparing trypsin solutions with different concentrations by using a phosphate buffer solution; (3) mixing the pretreated sheep tail meat paste with a buffer solution according to different solid-liquid ratios, and adding the trypsin solution; (4) carrying out heat preservation and enzymolysis in a constant-temperature water bath oscillator for several hours, then carrying out high-temperature enzyme deactivation, and centrifuging to obtain a separated oil phase; and (5) determining the quality of the extracted sheep tail oil. The method provided by the invention avoids the problems of easy discoloration, easy deterioration and incomplete extraction caused by the traditional baking method for extracting the sheep tail oil, and is a method which is simple to operate, low in cost, high in oil yield and high in quality of the extracted sheep tail oil.

The invention discloses a preparation method of phosphorylated peptidoglycan. The method is characterized by comprising the following steps of: inoculating Lactobacillus acidophilus on a culture medium for cultivation, centrifuging to collect thallus, repeatedly washing the thallus, suspending the thallus in hydrofluoric acid solution for one night at 4 DEG C, centrifugally collecting cell wall sediment, and washing the sediment to become neutral; dissolving the sediment into Trypsin chymotrypsin phosphate buffer solution, inactivating in boiling water, centrifuging the liquid supernatant, washing the sediment; dissolving the sediment in diethyl ether, stirring and centrifuging to collect sediment, and washing the sediment with water until completely removing the smell of diethyl ether, then obtaining the peptidoglycan; dissolving the peptidoglycan in Lysostaphin phosphate buffer solution for performing enzymolysis and centrifuging, taking the liquid supernatant, centrifuging the liquid supernatant, and centrifugally washing the sediment with water to get micromolecule peptidoglycan; and finally, dissolving the micromolecule peptidoglycan in mixed phosphate solution, precipitating with alcohol after reaction, treating the alcohol precipitated peptidoglycan by freeze drying and redissolving, and adding the redissolved solution in a dialysis bag, performing dialysis concentration, and then, performing freeze drying to get the phosphorylated peptidoglycan. The method has the advantages of increasing the solubility remarkably and improving the immunological effect to a certain extent.