Protein collection and enzymolysis integrated sample pretreatment apparatus and its production
A sample pretreatment and protein technology, applied in the direction of being fixed on/in an organic carrier, can solve the problems such as the enzyme column reactor is only suitable, the flow rate is difficult to match, the activity is decreased, etc., and achieves low cost, high speed, and production. simple method effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0024] Fabrication of a sample pretreatment device integrating protein collection and rapid enzymatic hydrolysis
[0025] 1) Production of the centrifuge tube: Drill a hole in the cover of the centrifuge tube b, the diameter of which is equivalent to that of the solid tube a.
[0026] 2) Preparation of matrix: using glycidyl methacrylate as a monomer and ethylidene dimethacrylate as a crosslinking agent to prepare a porous monolithic matrix. Weigh 0.6g of glycidyl methacrylate (GMA), 0.4g of ethylidene dimethacrylate (EDMA), dissolve in 1g of cyclohexanol / dodecanol (mass ratio: 50 / 50) mixed solvent, ultrasonically After 10 minutes, wrap the centrifuge tube containing the reaction solution with aluminum foil. Quickly weigh 1mg of photoinitiator DMPA and shake for 30s. Such as figure 1 As shown, take 0.8mL of the reaction solution c into the centrifuge tube b, insert the solid tube a, and put it into the ultraviolet crosslinking apparatus for photopolymerization for 10 minut...
Embodiment 2
[0032] 1) Production of the centrifuge tube: Drill a hole in the cover of the centrifuge tube b, the diameter of which is equivalent to that of the solid tube a.
[0033] 2) Preparation of the matrix: using glycidyl methacrylate as a monomer and ethylidene dimethacrylate as a crosslinking agent to prepare a porous monolithic matrix. Weigh 0.6g of glycidyl methacrylate (GMA), 0.4g of ethylidene dimethacrylate (EDMA), dissolve in 1g of cyclohexanol / dodecanol (mass ratio: 50 / 50) mixed solvent, ultrasonically After 10 minutes, wrap the centrifuge tube containing the reaction solution with aluminum foil. Quickly weigh 1mg of photoinitiator DMPA and shake for 30s. Such as figure 1 As shown, take 0.8mL of the reaction solution c into the centrifuge tube b, insert the solid tube a, and put it into the ultraviolet crosslinking apparatus for photopolymerization for 10 minutes.
[0034] 3) After taking out the solid tube a, add methanol to soak in the reaction device for 4 hours, the...
PUM
Property | Measurement | Unit |
---|---|---|
thickness | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com