Preparation method of sinonovacula constricta anti-hypertension peptide

A technology of high blood peptide and razor clam, which is applied in the field of preparation of anti hyperglycemic peptide of razor clam, and achieves the effects of green and safe anti high blood peptide, high precipitation rate, and less precipitation

Inactive Publication Date: 2017-05-10
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For razor clam constriction, there are still few studies on antihypertensive aspects, especially the antitumor studies on extracting polypeptides by enzymatic hydrolysis technology have not been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] A method for preparing anti-hyperglycemia peptide of razor clam, the specific operation steps are:

[0015] 1) Enzymatic hydrolysis: Rinse the razor clam with ice water 2 to 4 times, clean the sediment, dirt and microorganisms attached to the surface of the razor clam, and drain. Dry in a blower at a temperature of 50-70°C and a drying time of 1-2 hours, pulverize with a pulverizer, pass through a 100-400 mesh sieve, and add Tris-HCl buffer solution according to the volume ratio of material to liquid at 1:20-1:30, The concentration is 1.2~1.8 mol / L, heated and stirred in a water bath, and the pH is adjusted with hydrochloric acid. Add 6~12 parts of papain, 5~10 parts of neutral protease, 0.5~1.2 parts of cysteine, 0.05~0.08 parts of sodium sulfite, 0.2~0.5 parts of calcium chloride, 0.02 parts of ε-polylysine ~0.04 parts and 0.01~0.02 parts of thiamine and 4~12 parts of co-solvent sodium salicylate, 0.5~1.5 parts of thiobetaine and 0.1~0.3 parts of diallyl disulfide. ...

Embodiment 2

[0019] A method for preparing anti-hyperglycemic peptide of razor clam, the most preferred operation steps are:

[0020] 1) Enzymatic hydrolysis: Rinse the razor clam with ice water 3 times, clean the sediment, dirt and microorganisms attached to the surface of the razor clam, and drain. Dry in a blower at a temperature of 65°C and a drying time of 1.5 hours, pulverize with a pulverizer, pass through a 200-mesh sieve, add Tris-HCl buffer solution at a volume ratio of 1:25 to a concentration of 1.5 mol / L, and heat in a water bath Stir and adjust the pH with hydrochloric acid. Add 8 parts of papain, 6 parts of neutral protease, 0.6 parts of cysteine, 0.07 parts of sodium sulfite, 0.3 parts of calcium chloride, 0.02 parts of ε-polylysine, 0.01 parts of thiamine and water as a cosolvent 9 parts of sodium sylate, 1.0 part of thiobetaine and 0.3 part of diallyl disulfide. Using the compound enzyme preparation to enzymatically hydrolyze razor clams can not only obtain the anti-hype...

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PUM

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Abstract

The invention provides a preparation method of a sinonovacula constricta anti-hypertension peptide. The method comprises the following operation steps: (1) enzymolysis; (2) ultrafiltration; and (3) preparation of the sinonovacula constricta anti-hypertension peptide. The method has the following beneficial effects: sinonovacula constricta is subjected to enzymolysis by adopting a complex enzyme preparation, so that the anti-hypertension peptide can be obtained, the activity of the anti-hypertension peptide is not destroyed, the degradation speed is high and the reaction time is shortened; the solubility of the anti-hypertension peptide in a Tris-HCl buffer solution can be improved by adding a cosolvent and the extraction rate of the anti-hypertension peptide is improved; a hydration film around molecules of the anti-hypertension peptide can be weakened or disappear by a precipitation agent and the precipitation rate is high; the content of precipitated impure protein and polypeptide is low; the prepared sinonovacula constricta anti-hypertension peptide is high in purity, high in activity and good in anti-hypertension effect; and the sinonovacula constricta anti-hypertension peptide prepared by using an enzymolysis technology is green and safe and can be securely used for production of anti-hypertension drugs.

Description

technical field [0001] The invention relates to the technical field of polypeptide preparation, in particular to a method for preparing an antihypertensive peptide of razor clam constriction. Background technique [0002] Marine shellfish is rich in resources and rich in many physiologically active substances, so it has been widely studied and applied gradually. Constricted razor clam (Sinonovacula constricta), commonly known as razor clam, belongs to Petalbranchia, Heterodonta, Pleurophyllidae, Lighthouse Clamidae, and Constricted razor clam genus. It is distributed in the coastal areas of my country and has a large output. The meat is delicious and nutritious, rich in various essential amino acids, unsaturated fatty acids and other nutrients needed by human life activities. Constricted razor clam is cold in nature, can nourish yin and remove pathogenic heat, treat depression, cold dysentery, hot dysentery, women's postpartum weakness, deficiency and heat, etc., and has hig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/16A61P9/12
CPCC12P21/06C07K1/16C07K1/34C07K1/36
Inventor 黄芳芳余方苗唐云平杨最素丁国芳
Owner ZHEJIANG OCEAN UNIV
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