Preparation method and application of vitamin A and D extract in internal organs of pacific mackerel
A technology of fish viscera and vitamins, applied in the field of preparation of vitamin AD extract from Qingzhan fish viscera, to achieve high activity, mild enzymatic hydrolysis conditions, and thorough enzymatic hydrolysis
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Embodiment 1
[0018] The preparation method of the vitamin A D extract in the internal organs of Qingzhan fish, the specific operation steps:
[0019] 1) Pretreatment: clean the viscera of the herring, high-speed homogenization, and high-speed grinding to destroy the visceral cells of the herring, so that vitamins A and D in the cells flow out, the visceral particles become smaller, the specific surface area increases, and the reaction speed is accelerated;
[0020] 2) Enzymolysis: Add distilled water and enzymolysis agent to the homogenate. The composition and weight of the enzymolysis agent are divided into: 4~8 parts of trypsin, 2~3 parts of neutral protease, 0.5~1.5 parts of papain, 0.3~ 0.6 part of calcium chloride, 0.02~0.05 part of cysteine and 0.01~0.04 part of sugar alcohol acetate. The enzymatic hydrolysis conditions are mild and will not destroy the structure of vitamins A and D, so that they can maintain a high activity; the compound enzyme is combined with enzymatic hydrolysi...
Embodiment 2
[0025] The preparation method of vitamin A D extract in the internal organs of Qingzhan fish, the most preferred operation steps:
[0026] 1) Pretreatment: Clean 100 pieces of viscera of Qingzhan fish, homogenize at high speed, grind at high speed, destroy visceral cells of Qingzhan fish, let vitamin A and D in the cells flow out, the viscera particles become smaller, the specific surface area increases, and the reaction is accelerated speed;
[0027] 2) Enzymolysis: Add 300 parts of distilled water and enzymolysis agent to the homogenate. The composition of the enzymolysis agent and its optimal weight are divided into: 7 parts of trypsin, 2.5 parts of neutral protease, 1 part of papain, and 0.5 part of chlorinated Calcium, Cysteine 0.04 and Sugar Alcohol Acetate 0.02. The enzymatic hydrolysis conditions are mild and will not destroy the structure of vitamins A and D, so that they can maintain a high activity; the compound enzyme is combined with enzymatic hydrolysis, and t...
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