Preparation method and application of vitamin A and D extract in internal organs of pacific mackerel

A technology of fish viscera and vitamins, applied in the field of preparation of vitamin AD extract from Qingzhan fish viscera, to achieve high activity, mild enzymatic hydrolysis conditions, and thorough enzymatic hydrolysis

Inactive Publication Date: 2017-06-20
ZHEJIANG OCEAN UNIV
View PDF6 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still room for improvement in the extraction rate of this method and the purity and biological activity of the extracted vitamin A.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The preparation method of the vitamin A D extract in the internal organs of Qingzhan fish, the specific operation steps:

[0019] 1) Pretreatment: clean the viscera of the herring, high-speed homogenization, and high-speed grinding to destroy the visceral cells of the herring, so that vitamins A and D in the cells flow out, the visceral particles become smaller, the specific surface area increases, and the reaction speed is accelerated;

[0020] 2) Enzymolysis: Add distilled water and enzymolysis agent to the homogenate. The composition and weight of the enzymolysis agent are divided into: 4~8 parts of trypsin, 2~3 parts of neutral protease, 0.5~1.5 parts of papain, 0.3~ 0.6 part of calcium chloride, 0.02~0.05 part of cysteine ​​and 0.01~0.04 part of sugar alcohol acetate. The enzymatic hydrolysis conditions are mild and will not destroy the structure of vitamins A and D, so that they can maintain a high activity; the compound enzyme is combined with enzymatic hydrolysi...

Embodiment 2

[0025] The preparation method of vitamin A D extract in the internal organs of Qingzhan fish, the most preferred operation steps:

[0026] 1) Pretreatment: Clean 100 pieces of viscera of Qingzhan fish, homogenize at high speed, grind at high speed, destroy visceral cells of Qingzhan fish, let vitamin A and D in the cells flow out, the viscera particles become smaller, the specific surface area increases, and the reaction is accelerated speed;

[0027] 2) Enzymolysis: Add 300 parts of distilled water and enzymolysis agent to the homogenate. The composition of the enzymolysis agent and its optimal weight are divided into: 7 parts of trypsin, 2.5 parts of neutral protease, 1 part of papain, and 0.5 part of chlorinated Calcium, Cysteine ​​0.04 and Sugar Alcohol Acetate 0.02. The enzymatic hydrolysis conditions are mild and will not destroy the structure of vitamins A and D, so that they can maintain a high activity; the compound enzyme is combined with enzymatic hydrolysis, and t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a preparation method of a vitamin A and D extract in internal organs of pacific mackerel. The method comprises the following steps: 1) pretreatment; 2) enzymolysis; 3) extraction; and 4) purification. The method has the beneficial effects that complex enzymes carry out enzymatic hydrolysis in a combined way, and are activated by multiple enzyme activators, and the enzymatic hydrolysis speed is high and the enzymatic hydrolysis is more complete; microwaves help dissolution, and the dissolution rate of vitamin A and D is increased; the protein is removed by a mixed liquid of chloroform and ethanol, grease is removed by ethanol redissolution and purification, and the finally obtained vitamin A and D extract has a high extraction rate, high purity, and strong activity; the operation is simple, the cost is low, the economic value is high, and the prepared vitamin A and D extract can be used for preparing health care products or drugs or food.

Description

technical field [0001] The invention relates to the technical field of vitamin extraction, in particular to a preparation method and application of vitamin AD extract from viscera of medaka. Background technique [0002] Vitamin A, also known as retinol or anti-dry eye factor, is an unsaturated monohydric alcohol with an alicyclic ring, including vitamin A1 and vitamin A2 from animal food sources, and is a class of substances with retinol biological activity. Vitamin A1 is mostly stored in the liver of mammals and saltwater fish, and vitamin A2 is often stored in the liver of freshwater fish. Since the activity of vitamin A2 is relatively low, vitamin A is usually referred to as vitamin A1. Vitamin D is a sterol derivative with anti-rickets effect, also known as anti-rickets vitamin. At present, it is believed that vitamin D is also a steroid hormone, and the most important members of the vitamin D family are VD2 (ergocalciferol) and VD3 (cholecalciferol). Vitamin D is a ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07C403/08C07C401/00
CPCC07C403/08C07C401/00
Inventor 林琳
Owner ZHEJIANG OCEAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap