Method for refining sheep tail oil by using biological enzyme method

A biological enzyme method, sheep tail oil technology, applied in the production of fat, fat oil/fat production, fat oil/fat refining, etc., can solve the problems of perishable extraction, incompleteness, easy discoloration, etc., and achieve low cost and oil yield. The effect of high, low amount of enzyme

Pending Publication Date: 2021-10-15
满洲里双实肉类食品有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to solve the above problems, the present invention provides a method that can improve the problems of easy discoloration, easy deterioration and incomplete extraction caused by animal oil extraction, and is simple to operate, low in cost, can efficiently refine sheep tail oil and is suitable for the development of sheep tail oil and industrialized methods

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  • Method for refining sheep tail oil by using biological enzyme method
  • Method for refining sheep tail oil by using biological enzyme method

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Wash the obtained sheep tail part, remove the external connective tissue, cut into pieces of about 1 cm, grind it with a meat grinder, weigh 7 g of the ground sheep tail meat and place it in a 50 mL centrifuge tube for later use. Prepare a dipotassium hydrogen phosphate / potassium dihydrogen phosphate buffer solution with a pH of 8, and mix mutton tail meat with 28 mL of phosphate buffer solution at a material-to-liquid ratio of 1:4. Add a trypsin solution with a final concentration of 3.2% (w / v), mix well, and incubate the enzymolysis in a constant temperature water bath shaker at 50°C for 2h. After the reaction, heat to 90°C and keep for 10min to inactivate the enzyme. The centrifuge tube was centrifuged at a speed of 4000 rpm for 10 minutes to separate the extracted sheeptail oil. The sheeptail oil was clear and transparent, and the extracted sheeptail oil was weighed to be 5.65g, and the calculated extraction rate was 80.71%. The sheeptail oil obtained was detected b...

Embodiment 2

[0029] Wash the obtained sheep tail part, remove the external connective tissue, cut into pieces of about 1 cm, grind it with a meat grinder, weigh 7 g of the ground sheep tail meat and place it in a 50 mL centrifuge tube for later use. Prepare a dipotassium hydrogen phosphate / potassium dihydrogen phosphate buffer solution with a pH of 8, and mix mutton tail meat with 28 mL of phosphate buffer solution at a material-to-liquid ratio of 1:4. Add a trypsin solution with a final concentration of 0.8% (w / v), mix well, and incubate the enzymolysis in a constant temperature water bath shaker at 50°C for 2h. After the reaction, heat to 90°C and keep for 10min to inactivate the enzyme. The centrifuge tube was centrifuged at a speed of 4000 rpm for 10 minutes to separate the extracted sheeptail oil. The sheeptail oil was clear and transparent, and the extracted sheeptail oil was weighed to be 5.16g, and the extraction rate was calculated to be 73.71%. The obtained sheep tail oil is dete...

Embodiment 3

[0031] Wash the obtained sheep tail part, remove the external connective tissue, cut into pieces of about 1 cm, grind it with a meat grinder, weigh 7 g of the ground sheep tail meat and place it in a 50 mL centrifuge tube for later use. Prepare a dipotassium hydrogen phosphate / potassium dihydrogen phosphate buffer solution with a pH of 8, and mix mutton tail meat with 28 mL of phosphate buffer solution at a material-to-liquid ratio of 1:4. Add a trypsin solution with a final concentration of 0.32% (w / v), mix well, and incubate the enzymolysis in a constant temperature water bath shaker at 50°C for 4 hours. After the reaction, heat to 90°C and keep for 10 minutes to inactivate the enzyme. The centrifuge tube was centrifuged at a speed of 4000rpm for 10min to separate the extracted sheeptail oil. The sheeptail oil was clear and transparent, and the extracted sheeptail oil was weighed to be 5.01g, and the extraction rate was calculated to be 71.57%. The sheeptail oil obtained was...

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Abstract

The invention relates to a method for refining sheep tail oil by a biological enzyme method. The method comprises the steps of (1) pretreating raw materials; (2) preparing trypsin solutions with different concentrations by using a phosphate buffer solution; (3) mixing the pretreated sheep tail meat paste with a buffer solution according to different solid-liquid ratios, and adding the trypsin solution; (4) carrying out heat preservation and enzymolysis in a constant-temperature water bath oscillator for several hours, then carrying out high-temperature enzyme deactivation, and centrifuging to obtain a separated oil phase; and (5) determining the quality of the extracted sheep tail oil. The method provided by the invention avoids the problems of easy discoloration, easy deterioration and incomplete extraction caused by the traditional baking method for extracting the sheep tail oil, and is a method which is simple to operate, low in cost, high in oil yield and high in quality of the extracted sheep tail oil.

Description

technical field [0001] The invention relates to the technical field of biological separation and purification, in particular to the technical field of animal oil extraction methods, in particular to a method for refining sheeptail oil by a biological enzymatic method. Background technique [0002] Sheep can be divided into three categories according to the tail shape, namely fat tail sheep, thin tail sheep and fat hip sheep. Fat tail sheep means that the fat accumulates in the tail to form a cushion, with different shapes and sizes. The tail end above the hock is called short fat tail sheep, and the tail end below the hock joint is called long fat tail sheep. Mongolian sheep is a kind of short fat-tailed sheep. It is widely distributed in my country and has the largest number. It is now distributed in Inner Mongolia, Northeast China, North China and Northwest China, accounting for 44%. [0003] Mongolian sheep have strong physique, strong adaptability, and can withstand ext...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/02C11B3/16G01N30/02
CPCC11B1/02C11B1/025C11B3/16G01N30/02
Inventor 陈敬曹学君杨婷陈槿晶施振洲
Owner 满洲里双实肉类食品有限公司
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