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Method for preparing oligogalactomannose enzymatic hydrolysis solution from guar gum solution

A mannose enzymatic hydrolysis solution and mannanase technology are applied in the field of guar gum solution to prepare galactomannose oligosaccharide enzymatic hydrolysis solution, and can solve the problems of long time, low enzymatic hydrolysis efficiency, low oligosaccharide and the like, Achieve the effect of saving production time and cost, fast enzymatic hydrolysis and improving efficiency

Inactive Publication Date: 2018-02-16
QINGZHOU RONMER BIOLOGY TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One is that the existing method mainly adopts β-mannanase to the enzymatic hydrolysis of guar gum, but the efficiency of adopting a single enzyme to hydrolyze guar gum is not high
Although some existing technologies use the method of degrading plant polysaccharides with compound enzymes, since each enzyme degrades separately, it takes a long time and the enzymatic hydrolysis efficiency is low
The 2nd, in the guar gum enzymatic hydrolysis process, because the guar gum solution viscosity is too high, the concentration during the enzymolysis guar gum that generally adopts at present is 0.3-2%, the concentration of the oligosaccharide that obtains is lower, in further When preparing galactomannose powder, dehydration and drying treatment is required, which not only increases the cost of subsequent dehydration and drying of the product, galactomannose, but also seriously affects the efficiency of industrial production

Method used

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  • Method for preparing oligogalactomannose enzymatic hydrolysis solution from guar gum solution
  • Method for preparing oligogalactomannose enzymatic hydrolysis solution from guar gum solution
  • Method for preparing oligogalactomannose enzymatic hydrolysis solution from guar gum solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Take 12g of guar gum powder, dilute to 100mL with 0.2mol / mL citric acid-sodium citrate buffer solution, adjust the pH value to 6.0; then heat and swell at 92°C for 50min; after cooling to 25°C, At the same time, add β-mannanase 840U, xylanase 12000U, endoglucanase 24000U, mix well;

[0025] After adding the three enzymes, the temperature gradient enzymatic hydrolysis method is adopted, specifically:

[0026] (1) Raise the temperature from the cooled temperature to 35°C, and perform enzymatic hydrolysis for 1.5h;

[0027] (2) Raise the temperature to 48°C and perform enzymatic hydrolysis for 3 hours; increase the temperature by 1°C every 3 minutes during the heating process, and keep it for 10 minutes for every 1°C increase;

[0028] (3) Raise the temperature to 55° C. and perform enzymatic hydrolysis for 2 hours.

[0029] After the enzymatic hydrolysis, the oligogalactomannose enzymatic hydrolysis solution is obtained. The mass percentages of galactomannose oligosacc...

Embodiment 2

[0033] Take 13g of guar gum powder, dilute to 100mL with 0.2mol / mL citric acid-sodium citrate buffer solution, adjust the pH value to 6.3; then heat and swell at 93.5°C for 55min; after cooling to 28°C, At the same time, 1040 U of β-mannanase, 19500 U of xylanase and 32500 U of endoglucanase were added and mixed thoroughly.

[0034] After adding the three enzymes, the temperature gradient enzymatic hydrolysis method is adopted, specifically:

[0035] (1) Raise the temperature from the cooled temperature to 37°C, and perform enzymatic hydrolysis for 2 hours;

[0036] (2) Raise the temperature to 50°C and perform enzymatic hydrolysis for 3.5 hours; increase the temperature by 1.5°C every 4 minutes during the heating process, and keep it for 10 minutes for every 1.5°C increase;

[0037] (3) Raise the temperature to 55° C. and perform enzymatic hydrolysis for 2.5 hours.

[0038] After the enzymatic hydrolysis, the oligogalactomannose enzymatic hydrolysis solution is obtained. T...

Embodiment 3

[0042] Take 15g of guar gum powder, dilute to 100mL with 0.2mol / mL citric acid-sodium citrate buffer solution, adjust the pH value to 6.6; then heat and swell at 95°C for 60min; after cooling to 30°C, At the same time, 1350 U of β-mannanase, 30000 U of xylanase and 45000 U of endoglucanase were added and mixed thoroughly.

[0043] After adding the three enzymes, the temperature gradient enzymatic hydrolysis method is adopted, specifically:

[0044] (1) Raise the temperature from the cooled temperature to 40°C, and perform enzymatic hydrolysis for 2.5 hours;

[0045] (2) Raise the temperature to 53°C and perform enzymatic hydrolysis for 4 hours; during the heating process, increase the temperature by 2°C every 5 minutes, and keep it for 10 minutes for every 2°C increase;

[0046] (3) Raise the temperature to 55° C., and perform enzymatic hydrolysis for 3 hours.

[0047] After the enzymatic hydrolysis, the oligogalactomannose enzymatic hydrolysis solution is obtained. The mas...

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Abstract

The invention discloses a method for preparing galactomannose oligosaccharide enzymatic hydrolysis solution from a guar gum solution. First, a citric acid-sodium citrate buffer solution is used to prepare a guar gum solution, then the temperature and time are controlled for heating and swelling, and after cooling, the guar gum solution is prepared. At the same time, three enzymes, β-mannanase, xylanase, and endoglucanase, were added, mixed thoroughly, and the enzymatic hydrolysis temperature and time were controlled for degradation. The invention realizes the rapid enzymatic hydrolysis of guar gum under high concentration, obtains the galactomannose oligosaccharide enzymatic hydrolysis solution, and saves the cost of drying and purification in the subsequent production of galactomannose oligosaccharide.

Description

technical field [0001] The invention relates to a method for preparing an oligogalactomannose enzymatic hydrolysis solution from a guar gum solution, and belongs to the field of biotechnology. Background technique [0002] Guar gum is a natural thickener and quality improver processed from the endosperm of guar beans, an annual herbaceous leguminous crop. The main component is galactomannan. Guar gum is a white or yellowish free-flowing powder, soluble in cold or hot water, and forms a jelly-like substance when it meets water, which can thicken rapidly. The main chain of the galactomannan structure is mannan linked by β-(1-4) glycosidic bonds, and the branch chain is galactan linked by α-(1-6) glycosidic bonds. Galactomannose is an incomplete degradation product of galactomannan. It is a general term for oligosaccharides polymerized by 2-10 galactose and mannose molecules through glycosidic bonds. The molar ratio of galactose and mannose About 1:2. Galactomannose oligosac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14
Inventor 刘云国张亮荣宝刚
Owner QINGZHOU RONMER BIOLOGY TECH CO LTD
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