A preparation method of dipeptidyl peptidase-iv inhibitory peptide derived from Japanese yellow croaker swim bladder collagen

A dipeptidyl peptidase and swim bladder collagen technology is applied in the field of protein extraction, which can solve the problems of low resource utilization and achieve the effects of low addition amount, rapid enzymatic hydrolysis and improved dispersion.

Active Publication Date: 2022-01-18
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to overcome the problems of a large number of by-products and low resource utilization in the processing of Japanese croaker, the present invention provides a method for preparing dipeptidyl peptidase-IV inhibitory peptide derived from collagen in the swim bladder of Japanese croaker. The active peptide prepared by the invention has significant physiological activity and can be used in the fields of medicine, functional food, food, etc., so it has a good application prospect

Method used

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  • A preparation method of dipeptidyl peptidase-iv inhibitory peptide derived from Japanese yellow croaker swim bladder collagen
  • A preparation method of dipeptidyl peptidase-iv inhibitory peptide derived from Japanese yellow croaker swim bladder collagen
  • A preparation method of dipeptidyl peptidase-iv inhibitory peptide derived from Japanese yellow croaker swim bladder collagen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Pretreatment of Japanese yellow croaker swim bladder:

[0032] (a) Exhausting the swim bladder, freeze-drying, and crushing to a particle size of 15 μm to obtain frozen swim bladder powder;

[0033] (b) adding the frozen swim bladder powder obtained in step (a) into 0.2mol / L temperature-controlled alkaline ionic liquid bis-N-(2-diethylamino)ethyl polyethylene glycol bis-imidazole hydroxide salt, First, at 22°C, the ultrasonic power density is 0.25w / cm 2 , ultrasonic treatment for 15min; then at 14°C, ultrasonic power density 1.0w / cm 2 , ultrasonic treatment for 6min; finally at 6°C, ultrasonic power density 2.2w / cm 2 , sonicated for 2 minutes; centrifuged and dried to obtain fish maw powder after removing impurities and defatting;

[0034] (2) extract collagen with 5 wt% acetic acid combined with pepsin, and centrifugally dry to obtain Japanese yellow mackerel swim bladder collagen;

[0035] (3) Use pH-responsive gel factor to modify microbial protease to enzymo...

Embodiment 2

[0038] (1) Pretreatment of Japanese yellow croaker swim bladder:

[0039] (a) Exhausting the swim bladder, freeze-drying, and pulverizing to a particle size of 5 μm to obtain frozen swim bladder powder;

[0040](b) adding the frozen swim bladder powder obtained in step (a) into 0.05mol / L temperature-controlled alkaline ionic liquid bis-N-(2-diethylamino)ethyl polyethylene glycol bis-imidazole hydroxide salt, First, at 20°C, the ultrasonic power density is 0.2w / cm 2 , ultrasonic treatment for 10min; then at 15°C, ultrasonic power density 0.7w / cm 2 , ultrasonic treatment for 5min; finally at 4°C, ultrasonic power density 2.5w / cm 2 , sonicated for 1 min; centrifuged and dried to obtain fish maw powder after removing impurities and defatting;

[0041] (2) extract collagen with 5 wt% acetic acid combined with pepsin, and centrifugally dry to obtain Japanese yellow mackerel swim bladder collagen;

[0042] (3) Utilize the pH response gel factor modified microbial protease to enzy...

Embodiment 3

[0045] (1) Pretreatment of Japanese yellow croaker swim bladder:

[0046] (a) Exhausting the swim bladder, freeze-drying, and crushing to a particle size of 20 μm to obtain frozen swim bladder powder;

[0047] (b) adding the frozen swim bladder powder obtained in step (a) into 0.5mol / L temperature-controlled alkaline ionic liquid bis-N-(2-diethylamino)ethyl polyethylene glycol bis-imidazole hydroxide salt, First, at 25°C, the ultrasonic power density is 0.35w / cm 2 , sonicate for 10min; then at 10°C, ultrasonic power density 1.5w / cm 2 , sonicate for 8min; finally at 8°C, ultrasonic power density 2.5w / cm 2 , sonicated for 1 min; centrifuged and dried to obtain fish maw powder after removing impurities and defatting;

[0048] (2) extract collagen with 5 wt% acetic acid combined with pepsin, and centrifugally dry to obtain Japanese yellow mackerel swim bladder collagen;

[0049] (3) Utilize pH responsive gel factor modified microbial protease to enzymolyze the Japanese yellowt...

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Abstract

The invention relates to the technical field of protein extraction. In order to solve the problems of a large number of by-products and low resource utilization in the processing of Japanese croaker, a dipeptidyl peptidase-IV inhibitory peptide derived from collagen in the swim bladder of Japanese croaker is provided. The preparation method comprises the following steps: (1) pretreating the swim bladder of Japanese yellow croaker, then extracting collagen with 5wt% acetic acid combined with pepsin, and centrifugally drying to obtain the swim bladder collagen of Japanese yellow croaker; (2) using compound The enzymatic hydrolysis of the Japanese croaker swim bladder collagen obtained in step (1) to obtain the Japanese croaker swim bladder collagen peptide; (3) combining the Japanese croaker swim bladder collagen peptide with a dipeptidyl peptidase screening kit, namely Obtain dipeptidyl peptidase-IV inhibitory peptide. In the present invention, the dipeptidyl peptidase-IV inhibitory peptide is prepared by using the swim bladder of Japanese yellow croaker as the main raw material through a relatively efficient process. antihyperglycemic effect.

Description

technical field [0001] The invention relates to the technical field of protein extraction, in particular to a preparation method of dipeptidyl peptidase-IV inhibitory peptide derived from collagen in the swim bladder of Japanese yellow croaker. Background technique [0002] Diabetes has become the third metabolic disease that seriously threatens human health and life after malignant tumors and cardiovascular disease. It can cause various complications such as cerebrovascular disease, coronary heart disease, retinopathy, and even death. At present, the control and treatment of diabetes mainly rely on food control and drugs. Drugs for treating diabetes are mainly synthetic drugs, and they all have certain toxic and side effects. [0003] Dipeptidyl peptidase-4 (Dipeptidylpeptidase-IV, DPP-IV) in incretin drugs is a serine protease that can rapidly cleave and inactivate incretins, neuropeptides and cytokines. DPP-4 can specifically cut off the alanine or proline residue at th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 韩涛唐云平江晓霞丁国芳余方苗杨最素黄芳芳陈艳
Owner ZHEJIANG OCEAN UNIV
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