A preparation method of dipeptidyl peptidase-iv inhibitory peptide derived from Japanese yellow croaker swim bladder collagen
A dipeptidyl peptidase and swim bladder collagen technology is applied in the field of protein extraction, which can solve the problems of low resource utilization and achieve the effects of low addition amount, rapid enzymatic hydrolysis and improved dispersion.
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Embodiment 1
[0031] (1) Pretreatment of Japanese yellow croaker swim bladder:
[0032] (a) Exhausting the swim bladder, freeze-drying, and crushing to a particle size of 15 μm to obtain frozen swim bladder powder;
[0033] (b) adding the frozen swim bladder powder obtained in step (a) into 0.2mol / L temperature-controlled alkaline ionic liquid bis-N-(2-diethylamino)ethyl polyethylene glycol bis-imidazole hydroxide salt, First, at 22°C, the ultrasonic power density is 0.25w / cm 2 , ultrasonic treatment for 15min; then at 14°C, ultrasonic power density 1.0w / cm 2 , ultrasonic treatment for 6min; finally at 6°C, ultrasonic power density 2.2w / cm 2 , sonicated for 2 minutes; centrifuged and dried to obtain fish maw powder after removing impurities and defatting;
[0034] (2) extract collagen with 5 wt% acetic acid combined with pepsin, and centrifugally dry to obtain Japanese yellow mackerel swim bladder collagen;
[0035] (3) Use pH-responsive gel factor to modify microbial protease to enzymo...
Embodiment 2
[0038] (1) Pretreatment of Japanese yellow croaker swim bladder:
[0039] (a) Exhausting the swim bladder, freeze-drying, and pulverizing to a particle size of 5 μm to obtain frozen swim bladder powder;
[0040](b) adding the frozen swim bladder powder obtained in step (a) into 0.05mol / L temperature-controlled alkaline ionic liquid bis-N-(2-diethylamino)ethyl polyethylene glycol bis-imidazole hydroxide salt, First, at 20°C, the ultrasonic power density is 0.2w / cm 2 , ultrasonic treatment for 10min; then at 15°C, ultrasonic power density 0.7w / cm 2 , ultrasonic treatment for 5min; finally at 4°C, ultrasonic power density 2.5w / cm 2 , sonicated for 1 min; centrifuged and dried to obtain fish maw powder after removing impurities and defatting;
[0041] (2) extract collagen with 5 wt% acetic acid combined with pepsin, and centrifugally dry to obtain Japanese yellow mackerel swim bladder collagen;
[0042] (3) Utilize the pH response gel factor modified microbial protease to enzy...
Embodiment 3
[0045] (1) Pretreatment of Japanese yellow croaker swim bladder:
[0046] (a) Exhausting the swim bladder, freeze-drying, and crushing to a particle size of 20 μm to obtain frozen swim bladder powder;
[0047] (b) adding the frozen swim bladder powder obtained in step (a) into 0.5mol / L temperature-controlled alkaline ionic liquid bis-N-(2-diethylamino)ethyl polyethylene glycol bis-imidazole hydroxide salt, First, at 25°C, the ultrasonic power density is 0.35w / cm 2 , sonicate for 10min; then at 10°C, ultrasonic power density 1.5w / cm 2 , sonicate for 8min; finally at 8°C, ultrasonic power density 2.5w / cm 2 , sonicated for 1 min; centrifuged and dried to obtain fish maw powder after removing impurities and defatting;
[0048] (2) extract collagen with 5 wt% acetic acid combined with pepsin, and centrifugally dry to obtain Japanese yellow mackerel swim bladder collagen;
[0049] (3) Utilize pH responsive gel factor modified microbial protease to enzymolyze the Japanese yellowt...
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