Method for preparing butyl alcohol through fermenting by utilizing lignocellulose raw material
A lignocellulose and cellulose technology, applied in the field of biological fermentation, can solve the problems of high raw material cost, low butanol yield, consumption, etc., and achieves strong enzymatic hydrolysis adaptability, high reducing sugar yield, and good enzymatic hydrolysis effect. Effect
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Embodiment 1
[0030] Example 1 The preparation of Trichoderma viride F4 enzyme fermented liquid
[0031] Preparation of fermentation medium: wheat bran 10g, acid-treated corn cob 5g, microcrystalline cellulose 5g, CaCO 3 1.5g, yeast extract 4g, KH 2 PO 4 0.05g, CaCl 2 0.08g, MgSO 4 0.08g, ZnSO 4 ·7H 2 O 1.2 mg, MnSO 4 4H 2 O 0.5 mg, CoCl 2 0.4mg, add water to 200ml.
[0032] Preparation of seed liquid: put the above medium into a 500ml Erlenmeyer flask, sterilize at 121°C for 30 minutes, inoculate Trichoderma viride F4 at 10% (v / v) inoculation amount after cooling, and incubate at 28-33°C for 24 hours.
[0033] Liquid fermentation: Sterilize the above-mentioned medium at 121°C for 30 minutes, inoculate the prepared seed liquid into the medium at 10% (v / v) inoculation amount after cooling, add 1.5 g locust tree powder, cultivated for 72 hours. The filter paper enzyme activity of the fermentation broth reached 12.4IU / ml, and the average β-glucosidase activity was 1.7IU / ml.
Embodiment 2
[0034] The preparation of embodiment 2 Clostridium beijerinckii XH0906 seed liquid
[0035] Seed medium composition: peptone 10g / L, beef extract 6g / L, xylose 30g / L, sodium chloride 0.5g / L, ammonium sulfate 0.9g / L, iron sulfate 0.1g / L, magnesium sulfate 0.3g / L , Calcium chloride 0.1g / L, pH 7.0, sterilized at 121°C for 15min.
[0036] Clostridium beijerinckii ( Clostridium beijerinckii ) XH0906 is a fermentation strain. Scrape 1-2 rings of the preserved Clostridium beijerinckii from the slope and put it into the shake flask seed medium without feeding N 2 , 30 ℃ static culture for 36 h, to prepare the fermented seed liquid.
Embodiment 3
[0037] Example 3 Preparation of butanol by fermentation with corn stalks as raw material
[0038] (1) Pretreatment of corn stalks: take the crushed corn stalks and impregnate them with 2.0wt% dilute sulfuric acid with a solid-to-liquid ratio of 1g:2mL, and put them into the retainer of the steam explosion device at a temperature of 170°C and a pressure of 0.7MPa Keep it down for 5 minutes, and instantly release the pressure to explode, and get the corn straw pretreated by dilute acid steam explosion, which is mainly composed of xylose, cellulose and lignin, the dry matter concentration is 32%, and the xylose content is 21.2% (relative to dry substance), the cellulose content is 38.6%.
[0039] (2) Enzymatic hydrolysis of pretreated corn stalks: the corn stalks pretreated by dilute acid steam explosion were adjusted to pH 5.0 with NaOH. Add tap water to adjust the dry matter concentration to 5%, add Trichoderma viride F4 enzyme-producing fermentation broth, the addition amount...
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