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Method for preparing butyl alcohol through fermenting by utilizing lignocellulose raw material

A lignocellulose and cellulose technology, applied in the field of biological fermentation, can solve the problems of high raw material cost, low butanol yield, consumption, etc., and achieves strong enzymatic hydrolysis adaptability, high reducing sugar yield, and good enzymatic hydrolysis effect. Effect

Active Publication Date: 2017-07-18
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the following problems generally exist in the traditional acetone-butanol fermentation: (1) The traditional acetone-butanol fermentation strain needs to be cultivated and fermented under strict anaerobic conditions. If the operation is not careful, it is easy to enter the air, causing the bacteria to not grow normally. Therefore, it is usually necessary to pass inert gas such as N during the fermentation process. 2 (2) The yield of butanol is low, only about 20% (mass fraction), which makes the cost of raw materials in the butanol fermentation process relatively high, which restricts the development of the butanol fermentation industry; (3) ) Fermentation products have 40% by-products such as acetone and ethanol in addition to butanol, which consume limited carbon metabolic flow, reduce the proportion of butanol in the product, increase the difficulty of recovering butanol, and increase energy consumption
[0009] However, the acetone-butanol fermentation strain has a repressive effect on sugar metabolism, that is, it will preferentially utilize glucose in the presence of glucose, and other sugars such as xylose are hardly consumed
At the same time, the efficiency of Clostridium acetobutylicum in utilizing xylose metabolism is also low, and the rate of utilizing xylose for fermentation is much lower than that of glucose fermentation.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 The preparation of Trichoderma viride F4 enzyme fermented liquid

[0031] Preparation of fermentation medium: wheat bran 10g, acid-treated corn cob 5g, microcrystalline cellulose 5g, CaCO 3 1.5g, yeast extract 4g, KH 2 PO 4 0.05g, CaCl 2 0.08g, MgSO 4 0.08g, ZnSO 4 ·7H 2 O 1.2 mg, MnSO 4 4H 2 O 0.5 mg, CoCl 2 0.4mg, add water to 200ml.

[0032] Preparation of seed liquid: put the above medium into a 500ml Erlenmeyer flask, sterilize at 121°C for 30 minutes, inoculate Trichoderma viride F4 at 10% (v / v) inoculation amount after cooling, and incubate at 28-33°C for 24 hours.

[0033] Liquid fermentation: Sterilize the above-mentioned medium at 121°C for 30 minutes, inoculate the prepared seed liquid into the medium at 10% (v / v) inoculation amount after cooling, add 1.5 g locust tree powder, cultivated for 72 hours. The filter paper enzyme activity of the fermentation broth reached 12.4IU / ml, and the average β-glucosidase activity was 1.7IU / ml.

Embodiment 2

[0034] The preparation of embodiment 2 Clostridium beijerinckii XH0906 seed liquid

[0035] Seed medium composition: peptone 10g / L, beef extract 6g / L, xylose 30g / L, sodium chloride 0.5g / L, ammonium sulfate 0.9g / L, iron sulfate 0.1g / L, magnesium sulfate 0.3g / L , Calcium chloride 0.1g / L, pH 7.0, sterilized at 121°C for 15min.

[0036] Clostridium beijerinckii ( Clostridium beijerinckii ) XH0906 is a fermentation strain. Scrape 1-2 rings of the preserved Clostridium beijerinckii from the slope and put it into the shake flask seed medium without feeding N 2 , 30 ℃ static culture for 36 h, to prepare the fermented seed liquid.

Embodiment 3

[0037] Example 3 Preparation of butanol by fermentation with corn stalks as raw material

[0038] (1) Pretreatment of corn stalks: take the crushed corn stalks and impregnate them with 2.0wt% dilute sulfuric acid with a solid-to-liquid ratio of 1g:2mL, and put them into the retainer of the steam explosion device at a temperature of 170°C and a pressure of 0.7MPa Keep it down for 5 minutes, and instantly release the pressure to explode, and get the corn straw pretreated by dilute acid steam explosion, which is mainly composed of xylose, cellulose and lignin, the dry matter concentration is 32%, and the xylose content is 21.2% (relative to dry substance), the cellulose content is 38.6%.

[0039] (2) Enzymatic hydrolysis of pretreated corn stalks: the corn stalks pretreated by dilute acid steam explosion were adjusted to pH 5.0 with NaOH. Add tap water to adjust the dry matter concentration to 5%, add Trichoderma viride F4 enzyme-producing fermentation broth, the addition amount...

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Abstract

The invention discloses a method for preparing butyl alcohol through fermenting by utilizing a lignocellulose raw material. The method comprises the following steps: (1) pre-treating the lignocellulose raw material; (2) adopting Trichoderma viride F4 for performing enzymatic hydrolysis on the pre-treated raw material, thereby acquiring a hydrolyzed mixed liquid glucose of cellulose and hemicellulose, wherein the Trichoderma viride has been preserved at China General Microbiological Culture Collection Center on November 10, 2008 and the preservation No. is CGMCC No. 2736; (3) utilizing calcium hydroxide to perform detoxification treatment on the hydrolyzed mixed liquid glucose; (4) taking the detoxified liquid glucose as a carbon source, replenishing nutrient elements and preparing a fermenting culture medium; and (5) inoculating butylic zymophyte into the fermenting culture medium, fermenting and preparing butyl alcohol. According to the method disclosed by the invention, the cellulose and the hemicellulose can be simultaneously subjected to enzymatic hydrolysis, the hydrolysate is utilized as a fermentation substrate for preparing butyl alcohol and the method has the characteristics of good enzymatic hydrolysis effect, high fermentation yield, and the like.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a method for preparing butanol by fermenting lignocellulosic raw materials. Background technique [0002] Butanol is an important organic chemical raw material, which is widely used in chemical, pharmaceutical and petroleum industries. And because there are two more methylene groups than ethanol, butanol has higher hydrophobicity and lower volatility, can be mixed with gasoline in any proportion, and has a calorific value equivalent to gasoline. As a potential renewable bioenergy that can replace gasoline, butanol has attracted more and more attention from all over the world. [0003] With the increasing depletion of petroleum resources, reducing the proportion of fossil energy use and strengthening the development of renewable energy has become an important trend of human energy utilization in the future. Moreover, butanol produced by the propylene ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/16C12P19/14C12P19/02C12R1/145C12R1/885
CPCC12P7/16C12P19/02C12P19/14Y02E50/10
Inventor 张全曹长海关浩高慧鹏王领民乔凯
Owner CHINA PETROLEUM & CHEM CORP
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