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Protein enriching device

A protein and enrichment technology, applied in the protein field, can solve the problems of inability to use in a large range, the cost of the kit is high, and the process is complicated, and achieve the effects of low cost and energy consumption, good effect, and simple processing process.

Inactive Publication Date: 2018-04-13
湖北普罗金科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, most of the protein enrichment operations use kits or ultrafiltration centrifugation. Not only is the process complicated, the workload is heavy, and the cost of using kits is relatively high, so it cannot be used on a large scale.

Method used

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  • Protein enriching device

Examples

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Embodiment 1

[0016] Example 1, such as figure 1 As shown, a protein enrichment device is characterized in that it includes a housing 1, a water pump 2 and a foam separation device 3, and the housing 1 is provided with a crushing device 4 at the upper part and a centrifugal device 5 at the lower part, The crushing device 4 includes a ball mill 6 and a stirring device 7. The centrifugal device 5 is provided with an automatic switch cover 8. The centrifugal device 5 is a detachable device for easy cleaning. The upper end of the crushing device 4 is provided with an enzyme spraying device. device 9 and a driving device 10 for driving the ball milling device 6 and the stirring device 7; the upper end of the crushing device 4 is provided with a feed port 11, and the lower end is provided with a discharge port 13 with a first valve 12, and the feed port The lower part of 11 is connected to the ball milling device 6, and the ball milling device 6 is fixedly connected to the upper end of the pulver...

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Abstract

The invention discloses a protein enriching device. The protein enriching device mainly comprises a shell, a water drawing pump and a foam separation device, wherein a stirring device, a centrifugingdevice and a ball milling device are arranged in the shell and are used for extracting protein; the foam separation device is used for enriching the protein. Through the protein enriching device, liquid supernatant can be obtained by only adjusting a control device and a driving device to crush and centrifuge cells in raw liquid; the process is simple; the manpower and the time are saved; meanwhile, protein enriching liquid obtained by a foam separation method is excellent in effect, simple in treatment process, high in treatment capacity and low in cost and low in energy consumption.

Description

technical field [0001] The invention belongs to the technical field of proteins, in particular to a protein enrichment device. Background technique [0002] Protein separation and purification analysis is a research hotspot in the field of biological separation analysis in recent years. The analysis and identification of low-abundance proteins is one of the key and difficult points of proteomics research. Proteins that undertake important life activities in organisms are often Low-abundance proteins, however, their extremely low content brings difficulties to subsequent analysis and detection, and limits people's research and understanding of them. Therefore, the effective concentration of low-abundance proteins is the first priority for accurate analysis and identification one of the conditions. In fact, many aspects in the process of proteomics research involve the effective enrichment of samples. Foam separation is based on the principle of adsorption, bubbling into the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14
CPCC07K1/14
Inventor 葛峰熊倩杨明坤张珈洪斌李俊峰付帅张书晨
Owner 湖北普罗金科技有限公司
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