Lactobacillus gasseri strain and uses thereof

A technology of Lactobacillus gasseri and strains, applied in the field of medical microorganisms, can solve the problems of weak antagonism of pathogenic bacteria and difficult to meet the needs of clinical applications

Active Publication Date: 2014-07-09
XINLIFEI BIOMEDICAL TECH TIANJIN +1
View PDF3 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the antagonism of known exogenous bacteria to pathogenic bacteria is sti

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lactobacillus gasseri strain and uses thereof
  • Lactobacillus gasseri strain and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Example

[0034] Test Example 1

[0035] According to the method in the literature (16S rDNA rapid identification of contaminated bacteria in blood products, the establishment of a sequencing method, Ying Yanling et al., Chinese Journal of Microbiology and Immunology, 2009 Issue 10), the method of the Lactobacillus gasseri strain CGMCC No. The sequence of the 16S rDNA of .6362 was sequenced, and the sequencing results confirmed that the Lactobacillus gasseri strain of the present invention has the 16S rDNA sequence shown in SEQ ID NO:1.

Example Embodiment

[0036] Example 1

[0037] This example is used to illustrate the antagonistic effect of the strain of the present invention on Candida albicans. The Candida albicans used in this example is a strain with the trade number 10231 purchased from ATCC.

[0038] Cultivate Lactobacillus gasseri CGMCC No. 6362 with MRS liquid medium to a cell concentration of 1×10 7 The culture medium of CFU / mL is named culture medium A.

[0039] Cultivate Candida albicans with MRS liquid medium to a cell concentration of 1×10 7 The culture medium of CFU / mL is named culture medium B.

[0040] The above-mentioned culture solution A and the above-mentioned culture solution B were mixed in equal volumes as an experimental group, and the above-mentioned culture solution B alone was used as a control group.

[0041] Inoculate 1 mL of the experimental group and the control group culture medium into 150 mL of MRS liquid medium and incubate at 37° C. for 24 hours to obtain a co-cultured culture medium.

[0042] Take 10...

Example Embodiment

[0047] Example 2

[0048] This example is used to illustrate the antagonistic effect of the strain of the present invention on Escherichia coli. The Escherichia coli used in this example is a strain with the trade number 35218 purchased from ATCC.

[0049] Cultivate Lactobacillus gasseri CGMCC No. 6362 with MRS liquid medium to a cell concentration of 1×10 7 The culture medium of CFU / mL is named culture medium A.

[0050] Culture Escherichia coli in MRS liquid medium to a cell concentration of 1×10 7 The culture medium of CFU / mL is named culture medium B.

[0051] The above-mentioned culture solution A and the above-mentioned culture solution B were mixed in equal volumes as an experimental group, and the above-mentioned culture solution B alone was used as a control group.

[0052] Inoculate 1 mL of the experimental group and the control group culture medium into 150 mL of MRS liquid medium and incubate at 37° C. for 24 hours to obtain a co-cultured culture medium.

[0053] Take 100μL ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The present invention provides a strain, which is a Lactobacillus gasseri strain and is characterized in that the preservation number is CGMCC No.6362. The present invention further provides uses of the strain in preparation of drugs for prevention and/or treatment of vaginitis, preparation of vagina care products, and preparation of external genitalia sanitary products. With the technical scheme, antagonism effects of the Lactobacillus gasseri strain on multiple pathogenic bacteria can be achieved. In addition, the strain has high adhesion to vaginal epithelial cells. The strain can well meet practical clinical application requirements.

Description

technical field [0001] The invention relates to the technical field of medical microorganisms, in particular to a strain of Lactobacillus gasseri and the application of the strain. Background technique [0002] There are some dominant flora in the vagina of healthy women, and these dominant flora play a key role in maintaining the balance of normal vaginal flora. The dominant flora can kill pathogenic bacteria by secreting lactic acid, hydrogen peroxide, bacteriocin and other substances. At the same time, the competitive adhesion of epithelial cells in the vaginal mucosa acts as a biological barrier, preventing the reproduction of pathogenic bacteria in the vagina. [0003] However, the use of antibiotics, poor hygiene habits, the environment, radiotherapy and chemotherapy, mental stress and many other reasons can destroy the balance of normal vaginal flora, reduce the number of dominant flora, cause a large number of pathogenic bacteria to multiply, and lead to various vag...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20A61K35/74A61P15/02A61P31/02A61P31/04A61P31/10A61P33/02C12R1/225A61K35/747
CPCY02A50/30
Inventor 张文杰
Owner XINLIFEI BIOMEDICAL TECH TIANJIN
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap