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Lactobacillus crispatus strains and uses thereof

A technology of Lactobacillus crispatus and strains, applied in the field of medical microbiology, can solve the problems of difficult to meet the needs of clinical application, weak antagonism of pathogenic bacteria, etc.

Active Publication Date: 2016-04-13
浙江欣力菲生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the antagonism of known exogenous bacteria to pathogenic bacteria is still weak, and it is difficult to meet the actual clinical application requirements

Method used

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  • Lactobacillus crispatus strains and uses thereof
  • Lactobacillus crispatus strains and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] This example is used to illustrate the antagonistic effect of the strains of the present invention on Candida albicans. The Candida albicans used in this example is the strain with product number 10231 purchased from ATCC.

[0038] Lactobacillus crispatus CGMCCNo.6360 was cultured with MRS liquid medium to a cell concentration of 1×10 7 CFU / mL culture medium, named as culture medium A.

[0039] Candida albicans (Candida albicans) was cultured with MRS liquid medium to a cell concentration of 1×10 7 CFU / mL culture medium, named as culture medium B.

[0040] The above-mentioned culture solution A and the above-mentioned culture solution B were mixed in equal volumes as an experimental group, and the above-mentioned culture solution B alone was used as a control group.

[0041] 1 mL of the culture liquid of the experimental group and the control group were respectively inoculated into 150 mL of the MRS liquid medium, and cultured at 37° C. for 24 hours to obtain a co-cu...

Embodiment 2

[0048] This example is used to illustrate the antagonistic effect of the strain of the present invention on Escherichia coli (Escherichia coli). The Escherichia coli used in this example is the strain purchased from ATCC with the product number 35218.

[0049] Lactobacillus crispatus CGMCCNo.6360 was cultured with MRS liquid medium to a cell concentration of 1×10 7 CFU / mL culture medium, named as culture medium A.

[0050] Escherichia coli (Escherichiacoli) was cultured with MRS liquid medium to a cell concentration of 1×10 7 CFU / mL culture medium, named as culture medium B.

[0051] The above-mentioned culture solution A and the above-mentioned culture solution B were mixed in equal volumes as an experimental group, and the above-mentioned culture solution B alone was used as a control group.

[0052] 1 mL of the culture liquid of the experimental group and the control group were respectively inoculated into 150 mL of the MRS liquid medium, and cultured at 37° C. for 24 ho...

Embodiment 3

[0059] This example is used to illustrate the antagonistic effect of the strain of the present invention on Staphylococcus aureus (Staphylococcusaureus). The Staphylococcus aureus used in this example is the strain purchased from ATCC with the product number 25923.

[0060] Lactobacillus crispatus CGMCCNo.6360 was cultured with MRS liquid medium to a cell concentration of 1×10 7 CFU / mL culture medium, named as culture medium A.

[0061] Staphylococcus aureus (Staphylococcusaureus) was cultured with MRS liquid medium to a cell concentration of 1×10 7 CFU / mL culture medium, named as culture medium B.

[0062] The above-mentioned culture solution A and the above-mentioned culture solution B were mixed in equal volumes as an experimental group, and the above-mentioned culture solution B alone was used as a control group.

[0063] 1 mL of the culture liquid of the experimental group and the control group were respectively inoculated into 150 mL of the MRS liquid medium, and cult...

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Abstract

The present invention provides a strain, which is a Lactobacillus crispatus strain and is characterized in that the preservation number is CGMCC No.6360. The present invention further provides uses of the strain in preparation of drugs for prevention and / or treatment of vaginitis, preparation of vagina care products, and preparation of external genitalia sanitary products. With the technical scheme, antagonism effects of the Lactobacillus crispatus strain on multiple pathogenic bacteria can be achieved. In addition, the strain has high adhesion to vaginal epithelial cells. The strain can well meet practical clinical application requirements.

Description

technical field [0001] The invention relates to the technical field of medical microorganisms, in particular to a strain of Lactobacillus crispatus and the use of the strain. Background technique [0002] There are some dominant flora in the vagina of healthy women, and these dominant flora play a key role in maintaining the balance of normal vaginal flora. The dominant flora can kill pathogenic bacteria by secreting lactic acid, hydrogen peroxide, bacteriocin and other substances. At the same time, the competitive adhesion of epithelial cells in the vaginal mucosa acts as a biological barrier, preventing the reproduction of pathogenic bacteria in the vagina. [0003] However, the use of antibiotics, poor hygiene habits, the environment, radiotherapy and chemotherapy, mental stress and many other reasons can destroy the balance of normal vaginal flora, reduce the number of dominant flora, cause a large number of pathogenic bacteria to multiply, and lead to various vaginal d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/747A61P31/04A61P15/02A61L15/36C12R1/225
CPCY02A50/30
Inventor 张文杰
Owner 浙江欣力菲生物技术有限公司
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