Dual-mode fluorescent/magnetic resonance imaging contrast agent based on mesoporous silica and preparation thereof

A technology of mesoporous silica and magnetic resonance imaging, applied in the field of nano-biomedical materials, can solve problems such as affecting imaging effects, and achieve the effects of satisfying production and application, simple process, and good physical and chemical stability.

Inactive Publication Date: 2014-06-11
SHANGHAI NAT ENG RES CENT FORNANOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these functional substances are often embe

Method used

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  • Dual-mode fluorescent/magnetic resonance imaging contrast agent based on mesoporous silica and preparation thereof
  • Dual-mode fluorescent/magnetic resonance imaging contrast agent based on mesoporous silica and preparation thereof
  • Dual-mode fluorescent/magnetic resonance imaging contrast agent based on mesoporous silica and preparation thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Dissolve 0.001mol CTAB in 180 mL water, stir for 15 minutes, then add 6 mmol NaOH. Under vigorous stirring, add 0.01mol TEOS drop by drop, and slowly stir for 10 hours at 25°C. After aging at room temperature for 12 hours, the obtained reaction product is washed with double distilled water, and vacuum-dried at 80°C for 12 hours. , and finally calcined in air at 550°C for 6 hours. Take 1 g of the product and disperse it in 100 mL of toluene solution, add 10 mL of APTES, and reflux at 60°C for 24 hours. The product was washed three times with toluene, methanol and water respectively, and dispersed in water to obtain solution a.

[0028] Add 0.005 mol DTPA to 50 mL DMSO, after complete dissolution, add 0.005 mol NHS and EDC respectively, and stir at room temperature for 24 hours. Add solution a, stir again for 24 hours, add 0.005 mol Gd(NO 3 ) 3 ·6H 2 O. After stirring for 24 hours, the product was filtered, washed three times with absolute ethanol and water, and disp...

Embodiment 2

[0032]Dissolve 0.001mol CTAB in 180 mL water, stir for 15 minutes, then add 6 mmol NaOH. Under vigorous stirring, add 0.02mol TEOS drop by drop, and slowly stir for 10 hours at 25°C. After aging at room temperature for 12 hours, the obtained reaction product is washed with twice distilled water, and vacuum-dried at 80°C for 12 hours. , and finally calcined in air at 550°C for 6 hours. Take 1 g of the product and disperse it in 100 mL of toluene solution, add 30 mL of APTES, and reflux at 60°C for 24 hours. The product was washed three times with toluene, methanol and water respectively, and dispersed in water to obtain solution a.

[0033] Add 0.005 mol DTPA to 50 mL DMSO, after complete dissolution, add 0.005 mol NHS and EDC respectively, and stir at room temperature for 24 hours. Add solution a, and after stirring again for 24 hours, add 0.005 mol GdCl 3 ·6H 2 O. After stirring for 24 hours, the product was filtered, washed three times with absolute ethanol and water, an...

Embodiment 3

[0037] Dissolve 0.002mol CTAB in 180 mL water, stir for 15 minutes, then add 6 mmol NaOH. Under vigorous stirring, add 0.01mol TEOS drop by drop, and slowly stir for 10 hours at 25°C. After aging at room temperature for 12 hours, the obtained reaction product is washed with double distilled water, and vacuum-dried at 80°C for 12 hours. , and finally calcined in air at 550°C for 6 hours. Take 1 g of the product and disperse it in 100 mL of toluene solution, add 10 mL of APTES, and reflux at 60°C for 24 hours. The product was washed three times with toluene, methanol and water respectively, and dispersed in water to obtain solution a.

[0038] Add 0.004 mol DOTA to 50 mL DMSO, after complete dissolution, add 0.004 mol NHS and EDC respectively, and stir at room temperature for 24 hours. Add solution a, stir again for 24 hours, add 0.004mol Gd(NO 3 ) 3 ·6H 2 O. After stirring for 24 hours, the product was filtered, washed three times with absolute ethanol and water, and dispe...

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Abstract

The invention relates to a dual-mode fluorescent/magnetic resonance imaging contrast agent based on mesoporous silica and a preparation method thereof. The preparation method comprises the following steps: dissolving CTAB, and adding NaOH; dropwise adding TEOS; stirring, aging, washing, drying and roasting; dispersing a product in a toluene solution, adding APTES, refluxing, washing, dispersing through water to obtain a solution a, adding a chelating agent in DMSO, dissolving, separately adding NHS and EDC, stirring, adding the solution a, adding a gadolinium salt, filtering, washing, and dispersing with water to obtain a solution b; adding fluorescent molecules containing carboxyl in another DMSO, separately adding NHS and EDC, stirring, adding the solution b, stirring, filtering, washing, drying to obtain the dual-mode fluorescent/magnetic resonance imaging contrast agent based on mesoporous silica. The difunctional contrast agent has the advantages of small particle size, stable performance, good biocompatibility and strong fluorescence and magnetic resonance signals.

Description

technical field [0001] The invention relates to a method for preparing a fluorescence / magnetic resonance dual-mode contrast agent, in particular to a preparation technology for a dual-mode contrast agent which uses mesoporous silicon dioxide as a medium and simultaneously connects fluorescent molecules and gadolinium chelates. The invention belongs to the field of nano biomedical materials. Background technique [0002] Early diagnosis of tumors urgently needs a biological probe with multiple mode combination, high efficiency and specific active targeting. Magnetic resonance imaging and optical imaging are currently the most promising diagnostic modalities. Magnetic resonance imaging can be freely selected in the process of image acquisition, and has the advantage of providing spatial three-dimensional resolution images. It can scan almost any part of the human body, and can even be imaged only for certain tissue areas. This advantage is beneficial for the early detection ...

Claims

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Application Information

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IPC IPC(8): A61K49/08A61K49/00
Inventor 朱君周涓路青何丹农
Owner SHANGHAI NAT ENG RES CENT FORNANOTECH
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