A latex-enhanced immune turbidimetric kit for quantitative detection of procalcitonin pct

Abstract

The invention relates to a latex-enhanced immune turbidimetric kit for quantitatively detecting procalcitonin PCT. The kit includes R1 reagent: containing a protective agent, a reaction enhancer, a preservative and a buffer; R2 reagent: containing a protective agent, a preservative reagent, buffer and sensitized polystyrene latex particles coated with anti-human PCT antibody; and calibrator: containing protective agent, preservative, buffer and PCT recombinant protein; The ethylene latex particles are linked by streptavidin-biotin; the particle size of the latex particles in the R2 reagent is 40-500nm. The kit can be used for quantitative detection of PCT content in human blood on a biochemical analyzer and a scattering turbidimetric analyzer. The invention provides a convenient and fast PCT detection kit with high sensitivity, strong specificity and accurate quantification, which has strong instrument compatibility and low detection cost, and makes up for the clinical demand for PCT turbidimetric products.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a kit for quantitatively detecting procalcitonin PCT in human blood by using latex-enhanced immune turbidimetry. Background technique [0002] Procalcitonin (PCT) is the precursor substance of calcitonin CT, which is composed of 116 amino acids and has a molecular weight of 13kD. It is composed of three parts: N-terminal-calcitonin-C-terminal. The influence of hormone levels, the half-life in the human body is 25-30 hours, and has good stability. The main site of PCT production in the body is the liver, and other neuroendocrine cells such as peripheral blood mononuclear cells, spleen, lung or small intestine are also important sites for producing PCT. Endotoxin, cytokines can stimulate the release of PCT. [0003] As a clinical marker, PCT has unique advantages. The content of PCT in the blood of healthy people is extremely low (<0.05ng / ml), and 0.5ng / ml is con...

AI Technical Summary

Problems solved by technology

At present, the detection limit of various latex immunoturbidimetric detection products on the market is mostly limited to 0.1 μg / ml, and this detection limit is far from enough for the clinical application of procalcitonin turbidimetric products. Procalcitonin 0.5ng / ml is the cut-off value for the diagnosis of systemic infection (sepsis), and the PCT concentration in patients with lower respiratory tract bacterial infection may be lower than this cut-off value (≥0.25ng / ml and <0.5ng / ml), so the development sensitivity is higher PCT immunoturbidimetric products with stronger specificity, better stability and stronger anti-interference ability are still important problems to be solved in the field of clinical diagnostic product research

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