14 results about "Pro calcitonin" patented technology

The application provides a photochemical chemiluminescence detection reagent and a preparation method thereof, and a procalcitonin detection method. The photochemical chemiluminescence detection reagent comprises acceptor microspheres and donor microspheres; wherein the acceptor microspheres are carboxyl-modified acceptor microspheres, and the carboxyl is activated by sulfo-NHS / EDC and coupled with a procalcitonin antibody; and the donor microspheres are carboxyl-modified donor microspheres, and the carboxyl is activated by sulfo-NHS / EDC and coupled with a procalcitonin antibody. The photochemical chemiluminescence detection reagent has higher stability in various environments, can more accurately combine with target molecules, realizes more efficient detection and recognition, and enables the microspheres to more accurately capture and fix the target molecules, so as to perform subsequent luminescence reaction. The detection method has high sensitivity and a wide range, can be used as a detection reagent for diagnosing and identifying infectious diseases of individuals, and has application value.

This invention relates to the field of biomedical data processing technology, specifically to a stratification system for sepsis-related secondary invasive fungal infections based on laboratory data. The invention obtains the degree of static intestinal baseline damage by considering serum lactate concentrations at different historical testing times, temporal characteristics, and a preset maximum backtracking period; it obtains the cumulative dosage of antimicrobial drugs and the hourly cumulative distribution value of drug use based on the drug dosage distribution at different medication times within a neighborhood of the real-time trigger time; it obtains the overall degree of inflammation reduction and the hourly cumulative distribution value of inflammation regression based on the procalcitonin concentration distribution at testing times within a neighborhood; and it further obtains the net drug-induced intervention level. Based on the degree of static intestinal baseline damage and the net drug-induced intervention level, the fungal infection characteristics are stratified. This invention, by considering non-drug-induced interference, accurately obtains the comprehensive risk level of fungal infection, improving the effectiveness of fungal infection stratification.

The application discloses an inflammation infection compound quality control product and a preparation method and application thereof. The quality control product comprises a matrix liquid and inflammation markers. The matrix liquid contains human serum, a buffer, a protein protective agent, a plasticizing agent, amino acids, saccharides, a surfactant and a preservative. The inflammation markers include C-reactive protein, procalcitonin, serum amyloid A and interleukin-6. The quality control product is stable in performance, can be placed for 1 year under 2-8 DEG C and in the dark, and is suitable for combined detection of different inflammation items, is convenient for clinical operation, saves cost, and provides guarantee for the accuracy of clinical test results.

The invention provides a fusion polypeptide, a kit and a detection method for procalcitonin detection. The provided SCFV antibodies fused to the large subunit and the small subunit of luciferase respectively can provide the best detection effect. When the fusion polypeptide is used for detecting the procalcitonin or the analogue thereof, excellent clinical comparison correlation is achieved, the detection time is shorter, the detection speed is higher, and a matched instrument is simple in structure.

The invention discloses a chemiluminiscence detection kit for calcitonin. The chemiluminiscence detection kit comprises carboxyl magnetic nanoparticles coated by streptavidin, a calcitonin monoclonal antibody-1 labeled by acridinium ester, and a calcitonin monoclonal antibody-2 labeled by biotin. According to the chemiluminiscence detection kit for the calcitonin, the chemical substances, namely the cane sugar and the benzamidine hydrochloride, are added into the reagent, so that the influence of the procalcitonin in a sample on a test is reduced, and the stability and the specificity of the kit are greatly improved.

This invention discloses antibodies against procalcitonin or reagents and kits for detecting procalcitonin, relating to the field of antibodies. The anticalcitonin antibody disclosed in this invention includes a heavy chain complementarity-determining region and a light chain complementarity-determining region. This antibody provides an important source of raw materials for the detection of procalcitonin and exhibits good affinity or activity.

This invention discloses nucleic acid fragments, recombinant vectors, recombinant cells, and recombinant procalcitonin antigen, as well as their preparation methods and applications, relating to the field of procalcitonin preparation. The amino acid sequence of the recombinant procalcitonin antigen is shown in SEQ ID NO: 1, or the amino acid sequence of the recombinant procalcitonin antigen is obtained by attaching a protein purification tag to the C-terminus or N-terminus of the amino acid sequence shown in SEQ ID NO: 1. The recombinant procalcitonin antigen provided by this invention has higher expression levels and purity.

The application belongs to the technical field of microorganisms, and specifically discloses a fermented Lactobacillus muciapparius BLF03 and application thereof in resisting drug-resistant Staphylococcus aureus. The drug-resistant Staphylococcus aureus is Staphylococcus aureus resistant to drugs targeting beta-lactamase. The fermented Lactobacillus muciapparius BLF03 has been preserved in the China General Microbiological Culture Collection Center on November 6, 2025, and the preservation number is CGMCC No. 36521. The application provides the fermented Lactobacillus muciapparius BLF03 for resisting Staphylococcus aureus resistant to drugs targeting beta-lactamase, which can well relieve infection symptoms induced by drug-resistant Staphylococcus aureus, reduce the expression level of procalcitonin in the body after infection, and improve inflammatory stress response caused by infection, thereby providing a new safe and efficient strain selection for products resisting drug-resistant Staphylococcus aureus.

The invention discloses a time-resolved fluorescence immunoassay test strip for joint detection of procalcitonin (PCT) / interleukin-6 (IL-6) in peripheral blood as well as a preparation method and application of the time-resolved fluorescence immunoassay test strip. The technical field belongs to the technical field of biological detection, the existing lateral flow chromatography technology has the problems of narrow linear range, low specificity, poor accuracy, low sensitivity, poorer repeatability (interference) and the like, and aiming at the defects in the prior art, the technical scheme is as follows: a double-antibody sandwich method principle is combined with the characteristics of time-resolved fluorescent microspheres, and the time-resolved fluorescent microspheres are used for detecting the time-resolved fluorescent microspheres. Based on a lateral flow immunochromatography method, rapid, specific, accurate, sensitive, simple and convenient combined detection on PCT and IL-6 in peripheral blood is realized at the same time, and reproducibility is good.

The invention discloses a prediction model for early warning of leukemia sepsis, and belongs to the technical field of medical information. Immunosuppression and infection of leukemia patients are rapidly deteriorated, and existing indexes and models are low in sensitivity and difficult to recognize sepsis in time. According to the system, eight conventional indexes, namely C reactive protein, procalcitonin, neutrophil counting, lymphocyte counting, thrombin time, red blood cell counting, total bile acid and systolic pressure, of the first day of admission are extracted, key features are screened through single-factor logistic regression, LASSO and Boruta algorithms in a combined mode, a random forest model is trained, and 10-fold cross validation and grid search optimization are conducted; a nonlinear relation is analyzed by combining SHAP explanation and a restrictive cubic spline, AUC0.765 is trained, AUC0.700 is verified, individualized risk scoring is provided, a hospital information system is embedded, clinical early intervention is guided, and death is reduced.

The present application relates to the field of medical immunodetection, in particular to procalcitonin latex immunoturbidimetry detection reagent combination and application thereof. The present application provides procalcitonin latex immunoturbidimetry detection reagent combination comprising R1 reagent and R2 reagent and application thereof. The present application adopts latex immunoturbidimetry, and further provides procalcitonin latex immunoturbidimetry detection reagent combination with good stability, high precision, high accuracy, high anti-interference and high linear range level by optimizing reaction system and proportion of each component, which is conducive to the popularization and application of the reagent in clinic.

The present invention provides a diagnostic reagent which is suitable for nephelometric analysis with a simple photometer and has high sensitivity for the quantitative determination of procalcitonin in a sample, wherein the reagent is an aqueous suspension of polymer particles, wherein procalcitonin antibodies are covalently bound to the polymer particles, wherein even after a longer standing time no or only a very slight agglomeration / settling tendency is detected and the specific reactivity of the particles remains essentially constant, for which the present invention proposes that the average particle size of the suspended polymer particles is 150-450 nm, the suspension contains a proportion of a sugar or sugar alcohol which is dissolved therein in the range from 25-250 g / l and the pH of the suspension is 8-10.

The utility model provides a storage device for procalcitonin, which relates to the technical field of procalcitonin detection and comprises a protective frame, a support frame is fixed inside the protective frame, a group of support sleeves are slidably arranged on the top surface of the support frame, and a transmission component for driving the support sleeves to move is arranged inside the protective frame. According to the storage device for procalcitonin, a transmission wheel is rotated to enable a threaded rod to drive a movable sleeve to slide in a limiting sleeve, so that a movable frame moves in a supporting frame, the distance between adjacent supporting sleeves can be adjusted according to the storage requirement of test tubes, and when the distance between the adjacent supporting sleeves is increased, the test tubes can be conveniently stored. And when the distance between every two adjacent supporting sleeves is reduced, an operator can observe the adjacent test tubes conveniently, the storage device can be adjusted according to the lengths of different test tubes, and the stability of the storage device for placing the test tubes is improved.