81 results about "Pro calcitonin" patented technology

The invention relates to a chemiluminescence quantitative detection kit for procalcitonin, and a preparation method and detection method thereof. The kit comprises a procalcitonin series standard substance, a magnetic separation reagent (magnetic particle suspension coupled with streptavidin), a first reagent (anti-procalcitonin monoclonal antibody solution containing biotin N-hydroxysuccinimide ester label) and a second reagent (anti-procalcitonin monoclonal antibody solution containing alkaline phosphatase label). The sensitivity of the kit prepared from the magnetic particle suspension coupled with streptavidin, anti-procalcitonin monoclonal antibody solution containing biotin N-hydroxysuccinimide ester label and anti-procalcitonin monoclonal antibody solution containing alkaline phosphatase label is up to 0.008ng/ml; and the kit has the advantages of high accuracy, high precision, no need of prediluting the sample, and wide detection range, and is simple and time-saving to operate.

The invention relates to a test paper strip for (PCT/SAA) combined rapid detection of human procalcitonin/serum amyloid protein A. The test paper strip comprises a detecting card shell, a test strip, a sample pad, a gold conjugate pad coated with two colloidal gold labeled antibodies, a nitrocellulose membrane and water absorbing paper, wherein the nitrocellulose membrane is provided with a T1 detection line coated with a solid-phase matched anti-SAA monoclonal antibody, a T2 detection line coated with a solid-phase matched anti-PCT monoclonal antibody, and a control line C which is parallel to the detection line and coated with a goat anti mouse IgG monoclonal antibody. Two kinds of colloidal gold labeled antibodies are provided, which are respectively a colloidal gold labeled antibody which can be specifically combined with a to-be-detected antigen PCT and an antibody which can be specifically combined with a to-be-detected antigen SAA. The test paper strip disclosed by the invention can be used for simultaneously and rapidly detecting the PCT/SAA in a patient sample in a combined manner and has the advantages of improving the diagnosis accuracy of early inflammatory reaction of infectious diseases and being simple to operate, rapid and convenient.

The invention discloses a kit for detecting procalcitonin in the blood and a method for detecting the procalcitonin. The method includes the steps of 1), coating magnetic beads with streptavidin; 2), marking a first monoclonal antibody of the procalcitonin with biotin; 3), combining the magnetic beads coated with the streptavidin with the first monoclonal antibody of the procalcitonin, marked with the biotin for reaction to obtain a magnetic bead-SA-biotin-PCT monoclonal antibody solvent; 4), marking acridinium ester with a second monoclonal antibody of the procalcitonin to obtain an acridinium ester-PCT monoclonal antibody solvent; 5), allowing the procalcitonin in a sample or a calibration product to react with the acridinium ester-PCT monoclonal antibody solvent and the magnetic bead-SA-biotin-PCT monoclonal antibody solvent; 6), after cleaning, calculating content of the procalcitonin in the sample. By the arrangement, the problem that coming out of results takes a long time is solved, the kit and the method can be applied to a peripheral whole blood sample, and detection range is widened without reducing flexibility.

The invention discloses a procalciton inimmunofluorescence quantitation test strip and a preparation method thereof. Compared with a test strip prepared by a common stock solution, the procalcitonin immunofluorescence quantitation test strip prepared by the invention is excellent in storage stability and better in precision, the peaking of a detected sample has a higher signal and a smoother base line, and the accuracy is higher.

Specific antibodies against N-procalcitonin, peptides, genetic constructions and methods for the obtainment of the peptides used in the obtainment of the antibodies. These antibodies can be used for preparing drugs, or diagnostic kits for diseases that develop with systemic inflammatory response or metabolic stress.

The invention relates to fabrication method and application of a lead sulfide-cobalt oxide compound (PbS/Co3O4)-based photoelectric chemical procalcitonin sensor. Black titanium dioxide nanoparticle (B-TiO2 NPs), iodine oxygen bismuth nanosheet (BiOI NSs) and gold nanoparticle (Au NPs) are combined on indium tin oxide (ITO) conductive glass and are used as a substrate material, the light absorption range can be expanded by a formed sensitization structure, the photoproduction electron hole separation efficiency is facilitated, and meanwhile, the sensor is used for loading an antibody. The fabricated PbS/Co3O4 compound competes for a light source and a hole sacrifice agent with the substrate material, the signal reduction-type photoelectric chemical immunosensor is built, sensitive detection of the procalcitonin is achieved, and the method is of important significance to early diagnosis and monitoring of bacteria inflammatory disease infection.

The invention relates to the technical field of electrochemiluminescence immune sensors, and in particular relates to a preparation method and application of an immune sensor taking a cadmium sulfide and molybdenum disulfide nano compound (CdS/MoS2) as a luminescent material and a substrate material and taking potassium persulfate and hydrogen peroxide as a dual-co-reaction reagent for enhancing luminescent intensity. Semiconductor nano materials, namely CdS and MoS2, with similar band gaps are compounded, so that the electric conductivity and electron-hole separation efficiency can be increased; and K2S2O8 and H2O2, serving as co-reaction reagents, have synergistic effects, so that the luminescent intensity of the sensor can be increased, and the stability can be enhanced. Based on specific binding between antigen antibodies, the sensor is used for detecting procalcitonin (PCT); and according to the difference of PCT with different concentration to the obstruction degree of electron transfer, the sensor has different electrochemiluminescence intensity, and detection of PCT is realized.

The invention relates to the technical field of procalcitonin (PCT) detection, in particular to a detection kit for procalcitonin (PCT) and a preparation and application method thereof. A reagent R1 contains a buffer solution, potassium chloride, ethyl carbamate, 2-hydroxypropyl-Beta-cyclodextrin, sorbitol, PEG-20000, N, N-diethyl-p-octadecylsulfonimidopropylammonium propanesulfonate, and a preservative. A reagent R2 comprise a buffer solution, ethyl carbamate, 2-hydroxypropyl-Beta-cyclodextrin, sorbitol, rabbit anti-human calcitonin (PCT) antibody-coated latex particles (65nm), rabbit anti-human calcitonin (PCT) antibody-coated latex particles (145nm), rabbit anti-human calcitonin (PCT) antibody-coated latex particles (280nm), N, N-diethyl-p-octadecylsulfopropylammonium propanesulfonate, and a preservative. According to the detection kit for procalcitonin (PCT), the stability and the linear range of the reagent are remarkably improved and the sensitivity and accuracy of the reagentare remarkably enhanced.

The invention discloses an immunochromatographic test strip for quantitatively detecting procalcitonin and a quantitative detection method thereof. The test strip comprises a PVC lining plate, a nitrocellulose membrane (NC membrane), a combination pad, a sample pad and a water absorption pad; the PVC lining plate is sequentially overlapped and pasted with the sample pad, the combination pad, the NC membrane and the water absorption pad from left to right; the binding pad contains a nanogold labeled first antibody for resisting procalcitonin; the nitrocellulose membrane is provided with a detection area and a control area, fluorescent strips are respectively arranged in the detection area and the control area, a detection line is arranged on the fluorescent strip of the detection area, an anti-procalcitonin coated antibody is used as the detection line, a control line is arranged on the fluorescent strip of the control area, and a procalcitonin antigen is coated on the control line. Compared with a common method for detecting procalcitonin at present, the method has the advantages of simplicity and convenience in operation, quickness, convenience in carrying, small sample consumption and the like, and meanwhile, the linear range and the stability of detection are also improved.

The invention belongs to the field of clinical medicine detection, and discloses a quality control product of an inflammatory marker and a preparation method of the quality control product. The quality control product of the inflammation marker is composed of a matrix liquid and an inflammation marker component, wherein the matrix liquid is composed of a matrix, a 5-100 mmol/L buffer agent, a 0.01-5 g/L protein protective agent, a 5-100 g/L saccharide and a 0.05-5 g/L preservative; the inflammation marker comprises the following components: procalcitonin, C reactive protein, serum amyloid protein A, myeloperoxidase, interleukin-6 and lipoprotein related phospholipase. The quality control product of the inflammatory marker contains a plurality of inflammatory markers, so that the joint detection of different inflammatory items is realized. Meanwhile, commercial human serum or plasma is adopted as a matrix, the channel source is stable, large-batch production can be achieved, the clinical sample conformity degree is high, the universality is high, the matrix effect can be reduced to the maximum extent, and the uniformity, stability and other performance are good.

The invention relates to a detection reagent for procalcitonin colloidal gold by immunoturbidimetry, which can effectively solve the detection problem of procalcitonin. A technical scheme is characterized that the detection reagent comprises a reagent 1 and a reagent 2, the reagent 1 is prepared by the following steps: water is added in tromethamine and sodium azide, the materials are uniformly mixed, and hydrochloric acid is used for adjusting a pH value; and the reagent 2 is prepared by the following steps: water is added in tromethamine, bovine serum albumin, polyvinylpyrrolidone, polyethylene glycol 20000, tween-20, cane sugar, sodium azide, and casein, the materials are uniformly mixed, and hydrochloric acid is used for adjusting the pH value to obtain a buffer solution, the buffer solution is used for dissolving anti-human procalcitonin antibody-labeled colloidal gold particles, concentration is adjusted, and the reagent 2 is obtained. The detection reagent has the advantages ofscientific component, novel and unique characteristics, easy preparation, low cost, stable performance, long shelf-life, accurate testing, no environmental pollution, and obvious economic and social benefits, and is an innovation for the procalcitonin detection reagent.

The invention relates to a method for the diagnosis, prognosis, risk assessment, risk stratification, monitoring, therapy guidance and/or therapy control of an infectious disease in a subject, wherein said method comprises providing a sample of said subject; determining a level of matrix metalloprotease-8 (MMP-8) or fragment(s) thereof in a sample of said subject, wherein said level of MMP-8 or fragment(s) thereof distinguishes between the presence and absence of an infectious disease in a patient with symptoms of a systemic inflammatory condition. In a preferred embodiment the invention relates to the determination of procalcitonin (PCT) and MMP-8 and their combined use to distinguish between the presence and absence of infectious disease in patients with symptoms of systemic inflammatory condition. The invention also relates to a computer-implemented method and a kit for conducting the method of the invention.

The invention discloses fluorescent immunochromatographic test paper for detecting procalcitonin. The fluorescent immunochromatographic test paper comprises a PVC bottom plate, wherein a sample pad, acombination pad, a nitrocellulose membrane and a water absorption pad are sequentially arranged on the PVC bottom plate from left to right; one end of the sample pad is fixed on the PVC bottom plate,the other end of the sample pad is lapped on the combination pad, and a sample dripping hole is formed in the center of the sample pad; one end of the combination pad is fixed on the PVC bottom plate, and the other end of the combination pad is lapped on the nitrocellulose membrane; a detection line and a quality control line are sequentially arranged on the nitrocellulose membrane from left to right; and the left end of the water absorption pad is lapped on the nitrocellulose membrane. The fluorescent immunochromatographic test paper labels antibody proteins by means of time-resolved immunofluorescent microspheres, and carries out capture by using polyclonal antibodies, so that the detection sensitivity is higher; and the fluorescent immunochromatographic test paper greatly shortens thedetection time, is high in efficiency, and has good stability and repeatability.

The invention discloses a carbon nano-tube procalcitonin detection kit and a preparation method thereof. A test paper base plate is sequentially adhered with a sample pad, a carbon nano-tube colloidal gold pad, a nitrocellulose membrane and a water absorbent pad. The carbon nano-tube procalcitonin detection kit is characterized in that the carbon nano-tube colloidal gold pad is adhered with a carbon nano-tube colloidal gold compound labeled procalcitonin monoclonal antibody 1, and the nitrocellulose membrane is coated with a detection line and a quality control line of a procalcitonin monoclonal antibody 2. According to the kit disclosed by the invention, procalcitonin in a sample is detected by adopting a carbon nano-tube colloidal gold labelling technology so as to judge whether bacterial infection occurs. The carbon nano-tube procalcitonin detection kit disclosed by the invention has the characteristics of simplicity in operation, rapid reaction, high sensitivity, strong specificity and the like, and is suitable for field detection and self-detection.

The present invention relates to a diagnostic method for the identification of a subject suffering from a primary non-infectious disease having an increased risk of an adverse outcome potentially being induced by the administration of an antibiotic to said subject comprising the determination of the level of Procalcitonin (PCT) or a fragment thereof or a precursor or fragment thereof having a length of at least 12 amino acid residues in a sample of a bodily fluid from said subject and the correlation of the determined level to a potential risk induced by the administration of an antibiotic.

The invention discloses a procalcitonin and C-reactive protein combined detection kit. The kit comprises a kit body, a refrigeration mechanism, a control system, a reagent strip, a first detection object, a second detection object and a reaction buffer solution, the box body is divided into a first box body, a second box body and a third box body which are sequentially arranged in the length direction, the reagent strip, the first detection object and the second detection object are all stored in the first box body, the reaction buffer solution is stored in the third box body, the first detection object contains a biotin labeled PCT antibody complex and a fluorescently labeled Anti-chicken IgY antibody complex, the second detection object contains a fluorescently-labeled PCT antibody complex, and the reaction buffer solution contains a fluorescently-labeled CRP antibody complex and the fluorescently-labeled Anti-chicken IgY antibody complex. The precision of simultaneous detection of PCT and CRP is improved, and the stability of the detection reagent product is improved.

The invention discloses an immunoturbidimetry kit for determining procalcitonin in whole blood. The kit comprises a reagent R1, a reagent R2 and a procalcitonin calibrator, the reagent R1 comprises abuffer solution, a surfactant, a stabilizer, a sensitizer and a preservative, the reagent R2 comprises latex microsphere particles marked with procalcitonin antibodies, a buffer solution, a preservative and a stabilizer, and the procalcitonin calibrator comprises procalcitonin protein, a preservative, a buffer solution and a stabilizer. According to the immunoturbidimetry kit for determining procalcitonin in whole blood, a whole blood sample can be directly added into the kit for quantitative detection of procalcitonin; the whole blood sample does not need to be centrifuged and serum or plasmadoes not need to be separated, the equipment cost can be reduced, the detection efficiency can be improved, the reagent has a remarkable anti-interference effect on red blood cells in the whole bloodsample and is high in detection precision, and the detection value deviation is smaller than 10% compared with that of a scheme adopting serum or plasma for detection.

The invention discloses a novel immunoturbidimetric kit for detecting procalcitonin, and belongs to the technical field of detection reagents, a CE510 sealing agent in an R2 reagent can occupy blank sites on latex microspheres to avoid the agglutination phenomenon of the latex microspheres; according to the present invention, the tea saponin with a certain concentration is added to the R1 reagent, and the tea saponin is the strong surfactant and further has the lipid dissolving effect, such that the interference of different sample detection reagents at different degrees can be eliminated, and the combination of the monoclonal antibody and the target object cannot be interfered so as to ensure the credibility of the detection result; the kit adopts the monoclonal antibody, so that the batch-to-batch difference of the kit can be reduced. A latex enhanced immunoturbidimetric assay test shows that the labeled combined monoclonal antibody used in the latex enhanced immunoturbidimetric assay kit is highly consistent with a gold standard Rogowski electrochemical luminescence detection value, and the accuracy is far higher than that of an existing kit for labeling a polyclonal antibody.

The invention provides a device for judging sepsis infection conditions, and application thereof. According to the device, the input variables of the device comprise concentrations of C reactive protein, procalcitonin and interleukin-6, and the device for judging sepsis infection conditions is constructed by taking the concentrations of the three markers as the input variables; and the output variable of the device is mScore or mScoreplus. According to the invention, mScore or mScoreplus is used as a new index of sepsis infection conditions to judge the infection degree of a patient, so that the accuracy is high, bacterial infection and virus infection can be distinguished, clinicians can be helped to diagnose accurately, and the device is an effective tool for detecting infection, distinguishing bacterial infection and virus infection and monitoring diseases.