Immunofluorescence kit for quantitatively detecting content of troponin I and preparation method

Abstract

The invention discloses an immunofluorescence kit for quantitatively detecting the content of troponin I and a preparation method. The preparation method comprises the following steps: preparing a troponin antibody-large-particle fluorescent particle conjugate; preparing a nitrocellulose membrane labeled antibody; assembling a reagent card; preparing a TNI calibration product; establishing a standard curve; calculating the content of the troponin in a sample to be detected. The immunofluorescence kit for quantitatively detecting the content of the troponin I comprises a nitrocellulose membrane, a reagent and the calibration product. The lowest detection limit of the kit can reach 0.1ng/ml and the requirement of clinical application is met; the fastest time of a process from sample acquisition to detection result presentation only needs 10 to 15 minutes so that more expensive time for patients is provided, and illness states of the patients can be dynamically detected. The immunofluorescence kit for quantitatively detecting the content of the troponin I has good result relevance, no remarkable difference and an accurate and reliable detection result, and can be used for clinically replacing imported products; the detection cost is greatly reduced.

Description

technical field [0001] The invention belongs to the technical field of biological detection, in particular to an immunofluorescence kit for quantitative detection of troponin I content and a preparation method. Background technique [0002] Troponin is composed of three subunits of troponin T (TnT), troponin I (TnI) and troponin C (TnC), which are the main regulatory proteins of muscles. Because the three are tightly combined and calcium-dependent Sex, by the release of calcium to control muscle contraction function. Troponin I (TnI) is distributed in cardiomyocytes (cTnI) and mainly regulates the contraction of the myocardium. The concentration of cTnI in healthy people is lower than 20.4pg / ml. Because of its high specificity and high sensitivity, cardiac troponin I (cTnI) has become one of the highly sensitive and specific markers of myocardial injury, and it is also an important marker for judging myocardial injury, risk classification, and prognosis. Biochemical Indi...

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Problems solved by technology

[0004] The object of the present invention is to provide a kind of immunofluorescence reagent kit and preparation method for quantitatively detecting the content of troponin I, aiming at solving the problems of gel chromatography and high-performance liquid chromatography in the current method for detecting cTNI, which are time-consuming and difficult to automate; Poor accuracy, long operation time, low degree of automation, low sensitivity, generally only qualitative, not quantitative, especially the shortcoming of poor repeatability limits its clinical application, especially not suitable for accurate quantification to help treat Quantitative detection of body fluid marker proteins for disease diagnosis

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